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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 965 results for: form

Showing: 901 - 920 of 965 results

  2. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...proteins or viral proteins (VP1, VP2 and VP3) that form the icosahedral capsid of the virus. The ITR sequences...Sf9 cells , have also been developed. The newly formed viral particles produced in HEK293T cells are then...molecule that spontaneously reanneals upon infection, forming a double-stranded DNA (dsDNA) template. This eliminates... When these viral vectors co-infect a cell, they form concatemers and splice together, reconstructing ...capsid proteins VP1, VP2, and VP3, which together form the outer capsid shell that protects the viral genome...Recombinant adeno-associated viral (rAAV) vector Modified form of AAV used to deliver genetic material into cells...of experiments being performed. Your biosafety office can provide more information on your institution'...

  3. Institute for Protein Innovation

    Type
    Collection
    ... in the collection have been developed using a platform (Link opens in a new window) based on yeast display...

  4. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...all pX458 family plasmids. Additional Cloning Information Cloning Protocols Single guide cloning: Zhang...

  6. Mammalian RNAi Tools

    Type
    Collection
    ...step and proceed to binding of RISC. For more information on shRNA design and delivery, see resources below...

  7. E11 Bio PRISM Collection

    Type
    Collection
    ... Bio (Link opens in a new window) . For more information about PRISM, see the preprint: Park, S.Y., Sheridan...

  10. TALEN Guide

    Type
    Collection
    ...arrays in a 2012 issue of Nature Protocols . More information and help can be found at TALeffector Resources...

  11. Brain Armamentarium

    Type
    Collection
    ...specimens. These technologies have the potential to transform neuroscience research by bringing the precision...

  12. Molecular Biology Reference

    Type
    Guide
    ..., the nucleotides on the opposing strands of DNA form hydrogen bonds, where A always pairs with T, and...broadly in their functionality. In their simplest form, plasmids require a bacterial origin of replication...plasmid. The selectable marker is typically in the form of another antibiotic resistance gene under the ...fluorescence detection, and the sequence outputted in the form of a chromatogram. Sanger can sequence approximately...common is the Illumina NGS platform for short-read sequencing. This is the platform Addgene primarily uses...entire sequence using bioinformatic methods. It is important to note that NGS platforms in general do not ...The concept of genes as carriers of phenotypic information was introduced in the early 19th century by Gregor...

  13. The Pleiades Promoter Project

    Type
    Collection
    ...Promoter Plasmids using the table below. Additional information is available for each construct and can be accessed...

  14. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... is stable at 4°C for up to one year. The powder form of polybrene is stable at 4°C for several years....provided for your convenience. See “warranty information” in appendix. Table of Contents A. pLKO.1-TRC...Digesting pLKO.1 TRC-Cloning Vector C.4 Ligating and transforming into bacteria D. Screening for Inserts D.1 Recommended... TRC-Cloning Vector I.2 Recipes I.3 Warranty information Back to Top A. pLKO.1-TRC Cloning Vector A.1 ...pCMV-VSVG from Robert Weinberg’s lab. For more information, visit Addgene’s Mammalian RNAi Tools page. Several...Measure the DNA concentration. C.4 Ligating and Transforming into Bacteria Use your ligation method of choice...final volume Incubate at 16°C for 4-20 hours. Transform 2 μL of ligation mix into 25 μL competent DH5 ...

  15. Antibody Guide

    Type
    Guide
    ...light chains. These four domains come together to form a “Y” shape, as shown in Figure 1. The two arms ...the five human isotypes. Some isotypes naturally form multi-antibody units, while others exist as monomers...may want to identify or collect the protein in any form it may be present in. However, polyclonals can vary...antibody, for each antibody used. Blank controls in the form of sample buffer must also be run. Unique samples...fluorophores. Horseradish peroxidase (HRP) HRP performs a chemiluminescent reaction in the presence of...activated and its output measured. This method can be performed using the indirect detection method via a conjugated... able to recognize the protein in its native conformation. Attachment of the antibody to the beads is ...

  16. Validated gRNA Sequences

    Type
    Collection
    ... the following spreadsheet, fill out as much information as possible on the sequences, and email the file...

  17. Plan Your Experiment

    Type
    Guide
    ...potential consequences of having a non-functional form of your protein expressed in your system. HDR : ... to produce the Cas enzyme, and the enzyme then forms a complex with the gRNA for CRISPR activity. Because...purified Cas protein and in vitro transcribed gRNA to form a Cas-gRNA complex. You then delivery the RNP to... and large insertions or deletions. For more information on the wide range of CRISPR technologies, visit...Selecting the genetic manipulation you plan to perform will help you narrow down which components you’...edit, ideally less than 10 bp away. For more information, read our three tips to improve HDR efficiency...your Cas protein and gRNA, which you generally perform by chemical transfection or electroporation. Expression...

  18. Science Guides

    Type
    Guide
    ...hort P alindromic R epeat (CRISPR) systems, which form an adaptive immune system in bacteria, have been...genome engineering. Find essential background information on CRISPR and the basics for planning your first...

  19. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... tags such as fluorescent protein (FP) tags. Information on genes and associated neurodegenerative diseases...SOD1-VC SOD1 Venus CMV ALS Tiago Outeiro 89780 isoform PKC Gamma (JCE692) PRKCG PKC1 Spinocerebellar ataxia...

  20. Optogenetics Guide

    Type
    Guide
    ...from Arabidopsis thaliana is fused to truncated forms of cytosolic STIM1 to control calcium channels ~...UVR8 domains UVR8 plant photoreceptor protein that forms photolabile homodimers with slow reversal kinetics...the common actuators used in optogenetics. For information on sensors, check out our Biosensors Plasmid ...blue-shifted channelrhodopsin from Platymonas subcordiformis 445 CoChR Channelrhodopsin from Chloromonas...465 SwiChRca Chloride-conducting channel, Step-waveform inhibitory ChR with mutation C128A (derived from...widely used Cry2-CIB1 system, light induces a conformational change in cryptochrome Cry2 to permit CIB1 ...cryptochromes with light-induced changes in protein conformation/dimerization. In addition to the applications...
Showing: 901 - 920 of 965 results