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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 1,042 results for: ins;

Showing: 941 - 960 of 1042 results

  3. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...ml). NOTE: Phosphorylated oligos may be ordered instead. For this approach, the use of T4 PNK is omitted...type. While MEL cells are suspension cells, instructions for adherent cells have also been included. ... clones to a single 96-well plate that already contains 50 μl cell culture media per well for a final ...protein, perform an immunoblot using an antibody against the relevant protein 9 . References This protocol...

  4. Neuroscience

    Type
    Collection
    ...recombinant antibodies targeting neuroscience-related proteins....currently available from our catalog that target proteins popular in neuroscience research. These ready-... represent the official views of the National Institutes of Health....

  6. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...oligonucleotide (ssODN) template supplied by Ma et al., but instead from the mother’s wild type allele (Figure 1). ...

  7. Summer SciComm: Preprints

    Type
    Blog Post
    ...scientists can receive recognition for their efforts, inspire future research, and increase their impact. Open...

  13. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication...Publication Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids...

  14. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ... Plasmid Collections Fluorescent Proteins Michael Davidson Fluorescent Protein Collection...collection of ORFs tagged with a variety of fluorescent proteins. The majority of the ORFs are of mammalian origin...different fluorescent tags for you to create fusion proteins with your gene of interest. Please visit Michael...

  15. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert PI Publication...Publication Plant ID Plasmid Gene/Insert PI Publication Do you have suggestions for other plasmids that should...

  16. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...joining (NHEJ); a mechanism which frequently causes insertions or deletions (InDels) in the DNA, possibly resulting... with the Cas9 and gRNA plasmids, the cell may instead repair the break using homology-directed repair...introduce specific genomic changes. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors...

  17. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Vector Type Promoter PI Publication Empty gRNA ...

  18. Structural Genomics Consortium Plasmids

    Type
    Collection
    ... determine the three dimensional structures of proteins of medical relevance, and place them in the public...pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine tag and TEV cleavage...pFHMSP-LIC-C N/A Expression vector for secreted proteins with C-terminal hexahistidine tag and TEV cleavage...

  19. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...recommended that you determine if your cell type contains the target sequence before using any of these ...single species or PAM binding variant of Cas9. For instance, wild-type SpCas9 must be used with targets that...experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions...
Showing: 941 - 960 of 1042 results