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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 865 results for: SOM

Showing: 81 - 100 of 865 results

  1. Video Library

    Type
    Protocol
    ...standard plasmid transformation protocol and offer some tips and tricks to reduce transformation time and...microbiology, molecular biology or cell biology. Learn some of the key strategies of aseptic technique, including...

  2. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...the laboratory. Don’t mouth pipette! There may be some times when you may be working with a protocol that...titer prior to starting work in the laboratory. For some biohazardous waste, an autoclave or other method...

  3. Protocol - Bacterial Transformation

    Type
    Protocol
    ...important for other antibiotic resistances. Plate some or all of the transformation onto a 10 cm LB agar...your transformation procedure is working. TIP: Sometimes less is more. Although it may be counter-intuitive...

  5. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ... dissolved (but do not overboil the solution, as some of the buffer will evaporate and thus alter the ...Pro-Tip Remember, if you added EtBr to your gel, add some to the buffer as well. EtBr is positively charged...

  6. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...contains the plasmid DNA separated from bacterial chromosomes. Collect the supernatant into a new tube by pipetting...salt from the pellet which can cause problems with some common reactions. Air dry the pellet (can be done...

  7. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...or test multiple plasmids to find a suitable one. Some labs have reported better results when the plasmid...acceptable) Baseline removal: all samples will have some small amount of background signal that is most evident...

  8. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... select multiple target sequences for each gene. Some sequences will be more effective than others. In... viral infection. However, polybrene is toxic to some cell lines. In these cell lines, substitute protamine...

  11. Weighing Reagents Protocol

    Type
    Protocol
    ...appropriately sized container to put the reagent in. For some reagents, you might need to weigh it out in the ...

  12. Water Bath Protocol

    Type
    Protocol
    ...tubes and bottles of different sizes in the lab. Some water baths contain individual slots for heating...

  13. Gibson Assembly Protocol

    Type
    Protocol
    ...fragments can be assembled in one reaction. However, some labs have observed a sharp decrease in success rate...

  16. Immunocytochemistry

    Type
    Protocol
    ...methods such as methanol or acetone may be better for some applications. Remove the paraformaldehyde and follow...

  17. Colony Formation Titering Assay

    Type
    Protocol
    ...same time as the virus-mediated transduction. For some cell lines, transduction is optimized if the cells...
Showing: 81 - 100 of 865 results