We narrowed to 869 results for: SOM

... dissolved (but do not overboil the solution, as some of the buffer will evaporate and thus alter the ...Pro-Tip Remember, if you added EtBr to your gel, add some to the buffer as well. EtBr is positively charged...

...contains the plasmid DNA separated from bacterial chromosomes. Collect the supernatant into a new tube by pipetting...salt from the pellet which can cause problems with some common reactions. Air dry the pellet (can be done...

...or test multiple plasmids to find a suitable one. Some labs have reported better results when the plasmid...acceptable) Baseline removal: all samples will have some small amount of background signal that is most evident...

... select multiple target sequences for each gene. Some sequences will be more effective than others. In... viral infection. However, polybrene is toxic to some cell lines. In these cell lines, substitute protamine...

...sterile loop, toothpick or pipette tip to scrape some of the frozen bacteria off of the top. Do not let...

...manually call the correct base at the position. Sometimes an “N” is the result of an erroneous insertion...

...appropriately sized container to put the reagent in. For some reagents, you might need to weigh it out in the ...

...tubes and bottles of different sizes in the lab. Some water baths contain individual slots for heating...

...fragments can be assembled in one reaction. However, some labs have observed a sharp decrease in success rate...

... of methylation sensitive restriction sites . Sometimes enzymes cut sequences which are similar, but not...

...of colonies, but the reality is that it will have some amount of background. What you want to see is that...

...methods such as methanol or acetone may be better for some applications. Remove the paraformaldehyde and follow...

...same time as the virus-mediated transduction. For some cell lines, transduction is optimized if the cells...

...your donor and recipient plasmids. Because you lose some DNA during the gel purification step, it is important...

... product and recipient plasmid. Because you lose some DNA during the gel purification step, it is important...

...recombinant antibody preps that have a purity of ≥90% but some labs use unpurified tissue culture supernatant routinely...

...doled out to multiple people: someone to coordinate, someone to decide, someone to delegate, etc. If you find...course, many types of teams. Some teams may be relatively permanent; some teams may only need to exist...helpful, or sometimes even necessary, to build a team in the first place? Let’s think about some of the advantages... find that there is not someone (or someones) taking on these responsibilities on a team to which you ...by offering advice on areas where you might need some help. Today: Teamwork. My first experience on a ...undergrad at Worcester Polytechnic Institute. Though some WPI students chose to go the solo route for their... Fast forward nearly 20 years and I am known by some colleagues as the “cross-team guy” at Addgene, an...

...third chromosomes are the ones you’ll work with. If YFG is somehow on the Y or fourth chromosomes, you ...genome. They have four chromosomes: the X (and Y), second, third, and fourth chromosomes. Also, the fourth ...geneticists use balancer chromosomes to prevent genetic recombination. Balancer chromosomes essentially are messed...that males do not recombine chromosomes during meiosis. This can sometimes be a handy feature when setting...fourth and Y chromosomes are miniscule, so researchers generally do not manipulate them. Essentially, the X...material are contributed by mother and father). While some mutations can remain stable as homozygous alleles... messed up chromosomes with multiple inversions. They are unable to participate in genetic recombination...

... First, I will describe some of the many ways you can approach finding someone to give you advice and ...Second, I will offer some advice on how to “make the ask” once you have found someone you admire and want... you see someone who gives a great talk, ask them for advice on speaking. If you see someone with a great...connect you to. Sometimes, it is not who you know but who knows about you. I found some other great tips...circle. Asking someone to be your mentor Mentoring is a “loaded” word.  If you ask someone to be your mentor...relationships can last for an hour or for a year. Some click and some do not. These are all normal outcomes. If...whose career path you are interested in following? Someone you admire for their insight and way of expressing...

...targeted to lysosomes for degradation. Autophagy is a dynamic process which involves autophagosome synthesis...degraded to the lysosome, and degradation of autophagic substrates inside the lysosome. Historically, ... the acidic pH found in lysosomes. Therefore, the formation of autophagosomes leads to an increase of ...negative/RFP positive) upon fusion of an autophagosome with a lysosome. An increase in autophagy leads to more... puncta, while a block at fusion of autophagosomes with lysosomes leads to an increase in yellow puncta...autophagy focused on counting the number of autophagosomes. This approach, however, has inherent limitations...Rosella is targeted to the vacuole in yeast or the lysosome in mammalian cells. This decrease in pH quenches...