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...Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease...Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should be added...

...Genomic Tools for library construction, genomic modification, and cloning systems. Jump to Table: Cloning... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Back to Top Shuttle Vectors Search the table ... or sort by the table headings. Click on the publication link to view all other plasmids from that article...Description Vector Type Selectable Marker PI Publication Back to Top Do you have suggestions for other...

...expressed from plasmids. These antibodies undergo application-specific validation and consistent quality control...and provide experimental data for recommended applications. Addgene values transparency and accessibility...you choose and use antibodies, with an expert technical support team ready to answer your questions. All...antibodies developed and validated for many research applications. Browse Antibodies Production Interested in ... storage techniques, and explain common applications. View Guide Need Some Help? Browse some of our...

...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...Interfere Mammalian Bacteria Plant Yeast Resources Catalytically dead Cas9 (dCas9), or dCas9 fused to a transcriptional...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection...

...pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...Labs. ACEofBASEs (Link opens in a new window) Identification of sgRNA target sites and off-target evaluation... Target Finder (Link opens in a new window) Identification of gRNA target sequences from an input sequence...window) A collection of protocols, materials, and publications by members of the Division of Signaling and ...Download protocol Addgene CRISPR pooled library amplification CRISPR pooled libraries Protocol at Addgene ...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter ...Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...Marker PI Publication Return to top Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker ...Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...

...small biomolecules or other physiological intracellular processes with genetically encoded fluorescent biosensors...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Use this guide to learn more about the many applications of fluorescent proteins and to find plasmids...Microscopy Resource Center (Link opens in a new window) Leica Microsystems Resources for Microscopy in Life Sciences...

...optogenetics plasmids such as microbial opsins and optical switch tools to activate, inhibit, and manipulate...Optogenetics Plasmid Collection Microbial Opsin Plasmids Optical Switch Plasmids Additional Resources Optogenetics...proteins. These fusions can then be used as an optical switch to control protein interactions, dimerization...response spectra) of different types of opsins and optical switches available in these tables, refer to Addgene's... Eligible for Viral Packaging PI Return to top Optical Switch Plasmids Browse, sort, or search the table...

...unraveling DNA using its helicase properties, cleaving the targeted strand non-specifically along the way. Cas3...Promoter PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...top Plant ID Plasmid Gene/Insert Promoter PI Publication Return to top CRISPR Resources Addgene has a ...

...International Genetically Engineered Machine Foundation Registry of Standard Biological Parts - Repository...biology describes the rational engineering of biological systems for novel functions. Addgene is working...Browse plasmids related to cloning and genomic modification, including shuttle, integration, reporter, and...Fungal Algal Featured SynBio Deposits Alper Lab Y. Lipolytica Promoters Anderson Lab Plasmids and Phagemids...

...advantages and disadvantages depending on the application, and different luciferases require different ... course experiments, but is too dim for some applications. NanoLuc® is small (19 kDa), very bright, and... available, making it ideal for a variety of applications. To learn more and plan your experiment, read...Malcolm Moore 51819 Syn-ATP Firefly (modified) CMV Optical reporter of presynaptic ATP Timothy Ryan 24348 ...Engineered to use the substrate Akalumine with significantly brighter signal and emission in the near-infrared...OgNLuc ) fusions for BRET-based imaging and other applications. And see PalmGRET , a membrane-anchored version...

...] VGlut1 Rat Mouse IgG2a 128639 Anti-Versican [N351/23R] Versican Mouse Mouse IgG2a 128640 Anti-Pan-Ankyrin... Lgi1 Mouse Mouse IgG2a 177528 Anti-Brevican [N294A/10R] Brevican Rat Mouse IgG2a 177529 Anti-GluN1 [N308...ARHGAP4 Mouse Mouse IgG2a 190309 Anti-Brevican [N294A/6] Brevican Rat Mouse IgG2a 190311 Anti-NSD1 [N312...Ppp1r9a Mouse Mouse IgG2a 211585 Anti-Versican [N351/24R] Versican Rat Mouse IgG2a 211587 Anti-GluN3A/NR3A...Synaptotagmin-7 Mouse Mouse IgG2a 220429 Brevican [N294A/10R-2b] Brevican Rat Mouse IgG2a 220430 BAF53b [N332B...external) Human Mouse 206772 Brevican scFv [N294A/6] N294A/6 scFv Brevican Rat Mouse 206773 GluN3A/NR3A...extensively validated for neuroscience research applications. A substantial number of these have been cloned...

... a prime editing guide RNA (pegRNA). As with a typical gRNA, the pegRNA is designed with a spacer that...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter ...Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...Marker PI Publication Return to top Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable...

...K-RAS, N-RAS, RASH1 ICAM1 intercellular adhesion molecule 1 BB2, CD54, P3.58 ICAM2 intercellular adhesion...repurposing of open access immunological assay data for translational and clinical research. Sci Data. Feb...and adaptive immune signaling and cell-cell communication. Plasmid...specific to any particular pathogen. It includes physical barriers such as the skin and mucosa, antimicrobial...molecules that enable cells of the immune system to communicate with each other across long distances. During...with virus and, like other cytokines, enable communication between cells of the immune system. Interleukins...are secreted by immune cells and coordinate communication between immune cells over the course of an immune...

...into a genomic attP site. So far, their practical application has been limited by low integration efficiency...other recombinase systems. Enzyme Target Site Canonical Sequence Structure (Length) Common Variants Cre...repeats + 4 bp spacer (32 bp) Currently, only the canonical rox site is widely used. Flp FRT GAAGTTCCTATTCCGAAGTTCCTATTCtctagaaaGTATAGGAACTTC...excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre...

... Promoter PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter PI Publication Return to top...Promoter PI Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter PI Publication Return to...

...excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases...doubly-specified combination of genetic and/or anatomical-defined parameters, by placing two orthogonal...fragments (which have become exons) determines the logical expression requirements of Cre and Flp (B,E). When...Robertson K, Osten P, Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal Circuits through the Parafascicular...

...Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert PI Publication Return to top ...cleaved the target RNA sequence, they adopt an enzymatically active state and can bind and cleave additional...top Plant ID Plasmid Gene/Insert PI Publication Return to top CRISPR Resources Addgene has a large selection...

...cited in publications. All pricing includes DNA amplification, viral vector production, purification, and ...Additional Resources Addgene's Data Hub Curated technical reports from the research community demonstrating...

...pX458 plasmid family. They have made several modifications to the the pX458 plasmid to expand its usefulness...developmental studies. Key plasmid features and pX458 modifications: replacement of the Cbh promoter with EF1alpha...Plasmids from Collection ID Plasmid Description PI Publications Kaufman, M. L., Goodson, N. B., Park, K. U.,... alters photoreceptor and bipolar cell fate specification, yet differs from deleting both genes . Development...