...Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID...Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty Prime...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled... a prime editing guide RNA (pegRNA). As with a typical gRNA, the pegRNA is designed with a spacer that...

...horizontal stripes on the sides of the adult body, is a tropical freshwater fish in the minnow family. It has long...inflammation and infection, regeneration, human neurological disorders, microbiota studies, and drug discovery...Cell lineage tracing in multicellular systems typically relies on genetic markers, somatic mutations, ...types. CreLite - George Eisenhoffer Lab. An optogenetically controlled Cre/loxP system that enables precise...the international research community. The Latin American Zebrafish Network (LAZEN) - LAZEN aims to increase...advanced training of young scientists in Latin America. The Zebrafish Book - A guide for the laboratory...

...Capsids AAV Packaged on Request AAV Guide Plasmids Publications Enhancer AAV plasmids are plasmids which use...following regions: Isocortex Hippocampal formation Cortical subplate Striatum Pallidum Hypothalamus Cerebellum...pAAV-AiE2051m-minBG-SYFP2-WPRE3-BGHpA AiP1277 AiE2051m SYFP2 Claustrum Cortical subplate 220711 pAAV-AiE2051m-minBG-iCre(R297T...R297T)-BGHpA AiP1786 AiE2051m Cre Claustrum Cortical subplate 230518 pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA ...pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA AiP1211 AiE2051m FlpO Claustrum Cortical subplate 191720 pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA ...= Substantial non-specific labeling observed Publications Ben-Simon Y, Hooper M, Narayan S, Daigle T, ...and transgenic mouse lines for genetic access to cortical cell types. bioRxiv [Preprint]. 2024 Sep 26:2024.06.10.597244...

... expression, transgenics and genome modification Species-Specific Expression If you want to drive...His or optimized YFP tags for purification or localization experiments Yeast...for bacterial expression (Gateway) GST Protein Purification pEBG - N-terminal GST for mammalian... the plasmid. Selectable Marker Typical Host Organism Representative Empty ... packaging and expression. See our dedicated lentiviral plasmid page . Retroviral Easy and ...shRNA expression, transgenics and genome modification Element Details Representative Empty ...

...Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease...Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should be added...

...Genomic Tools for library construction, genomic modification, and cloning systems. Jump to Table: Cloning... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Back to Top Shuttle Vectors Search the table ... or sort by the table headings. Click on the publication link to view all other plasmids from that article...Description Vector Type Selectable Marker PI Publication Back to Top Do you have suggestions for other...

...expressed from plasmids. These antibodies undergo application-specific validation and consistent quality control...and provide experimental data for recommended applications. Addgene values transparency and accessibility...you choose and use antibodies, with an expert technical support team ready to answer your questions. All...antibodies developed and validated for many research applications. Browse Antibodies Production Interested in ... storage techniques, and explain common applications. View Guide Need Some Help? Browse some of our...

...Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert PI Publication Plant ID Plasmid Gene...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...cleaved the target RNA sequence, they adopt an enzymatically active state and can bind and cleave additional...Gene/Insert PI Publication Do you have suggestions for other plasmids that should be added to this list?...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...using any of these gRNA plasmids and review the publication associated with each plasmid for more information...upstream of a 5' NGG 3' PAM sequence. Which CRISPR application is this gRNA sequence compatible with? CRISPR...CRISPR nuclease and function. Please see the publication or plasmid information page, or contact the depositing...plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

... Tools for Affinity Purification These AAV encode tools for affinity purification (which can be used for...tet-off transactivators and tools for affinity purification (TRAP). Viral... Tools Tetracycline Transactivators Affinity Purification Neurophysiology Cell Ablation Tetracycline Transactivators...of the voltage-gated Na+ channel mNaChBac and (physically separate) tdTomato 8 Scanziani Tools for Cell...

...] VGlut1 Rat Mouse IgG2a 128639 Anti-Versican [N351/23R] Versican Mouse Mouse IgG2a 128640 Anti-Pan-Ankyrin... Lgi1 Mouse Mouse IgG2a 177528 Anti-Brevican [N294A/10R] Brevican Rat Mouse IgG2a 177529 Anti-GluN1 [N308...ARHGAP4 Mouse Mouse IgG2a 190309 Anti-Brevican [N294A/6] Brevican Rat Mouse IgG2a 190311 Anti-NSD1 [N312...Ppp1r9a Mouse Mouse IgG2a 211585 Anti-Versican [N351/24R] Versican Rat Mouse IgG2a 211587 Anti-GluN3A/NR3A...Synaptotagmin-7 Mouse Mouse IgG2a 220429 Brevican [N294A/10R-2b] Brevican Rat Mouse IgG2a 220430 BAF53b [N332B...external) Human Mouse 206772 Brevican scFv [N294A/6] N294A/6 scFv Brevican Rat Mouse 206773 GluN3A/NR3A...extensively validated for neuroscience research applications. A substantial number of these have been cloned...

...incorporate a non-canonical amino acid into the protein of interest: The non-canonical amino acid, which...They are typically expressed from a single plasmid, with multiple copies of the tRNA. Applications By making...aaRS) that has been evolved to incorporate non-canonical amino acids (ncAAs). A plasmid containing the ...the gene of interest with the modified codon (typically the amber codon) that is recognized by the cognate...plasmids have been introduced in the cells, the non-canonical amino acid can be incorporated using the existing...

...repository contains many plasmid tools that were specifically designed for use in plants. These resources ...Multipurpose Toolkit enables targeted, specific modification of monocot and dicot genomes using genome engineering...MoClo Plant Parts II and Infrastructure Kit for hierarchical assembly of plant multigene constructs - Johannes...an Old Favorite External Resources Arabidopsis Biological Resource Center (ABRC) (Link opens in a new window...Taproot is a podcast to understand how scientific publications are created. Papers from the plant biology literature...

...K-RAS, N-RAS, RASH1 ICAM1 intercellular adhesion molecule 1 BB2, CD54, P3.58 ICAM2 intercellular adhesion...repurposing of open access immunological assay data for translational and clinical research. Sci Data. Feb...and adaptive immune signaling and cell-cell communication. Plasmid...specific to any particular pathogen. It includes physical barriers such as the skin and mucosa, antimicrobial...molecules that enable cells of the immune system to communicate with each other across long distances. During...with virus and, like other cytokines, enable communication between cells of the immune system. Interleukins...are secreted by immune cells and coordinate communication between immune cells over the course of an immune...

...excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases...doubly-specified combination of genetic and/or anatomical-defined parameters, by placing two orthogonal...fragments (which have become exons) determines the logical expression requirements of Cre and Flp (B,E). When...Robertson K, Osten P, Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal Circuits through the Parafascicular...

...Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Selectable Marker PI Publication Last reviewed...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...bidirectional insertions and may not be suitable for applications where orientation matters. Type V-K systems ...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium (cryptosporidiosis...leishmaniasis), and others have been the focus of biomedical research for decades. Recent developments with...NHEJ. Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma CRISPR Knockout Pooled Library...

...pX458 plasmid family. They have made several modifications to the the pX458 plasmid to expand its usefulness...developmental studies. Key plasmid features and pX458 modifications: replacement of the Cbh promoter with EF1alpha...Plasmids from Collection ID Plasmid Description PI Publications Kaufman, M. L., Goodson, N. B., Park, K. U.,... alters photoreceptor and bipolar cell fate specification, yet differs from deleting both genes . Development...

...SARS-CoV-2 infection remain poorly understood but are critical for developing therapeutic strategies to prevent...vesicular stomatitis virus G) are packaged as replication-defective virus particles with the SARS-CoV-2...protein is inefficient for pseudotyping; several modifications may enhance infectious particle production, ...particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description Mutations...

...ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a transcriptional...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression...