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We narrowed to 1,064 results for: tra

Showing: 121 - 140 of 1064 results

  1. AAV Packaged on Request

    Type
    Collection
    ...of the applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the ...and useful gene delivery tools that effectively transduce many mammalian cell types. AAV is used in a wide...service lets scientists choose from over 4,000 transfer plasmids to be packaged in their choice of seven...chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids in our repository are eligible for this...aliquots 1.0 mL Large 25 × 100 µL aliquots 2.5 mL Extra Large 50 × 100 µL aliquots 5.0 mL *AAV2 yields are...several important steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation...experiments. Shipping 2–7 days We will email you with tracking information as soon as your order ships. Shipping...

  2. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pcDNA3-HtrA2-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A HTRA2 GFP CMV...pcDNA3-delta133HtrA-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14126 pET20b-delta133HtrA2 HTRA2 His...pcDNA3-HtrA2-FLAG HTRA2 Flag CMV Parkinson's L. Miguel Martins 15939 pcDNA3-HtrA2-FLAG S142D HTRA2 Flag ...pcDNA3-HtrA2-FLAG S400D HTRA2 Flag CMV Parkinson's L. Miguel Martins 16153 pET20b-delta133HtrA2 S142A ...S142A HTRA2 His T7 Parkinson's L. Miguel Martins 16154 pET20b-delta133HtrA2 S142D HTRA2 His T7 Parkinson's...pET20b-delta133HtrA2 S400A HTRA2 His T7 Parkinson's L. Miguel Martins 16156 pET20b-delta133HtrA2 S400D ...S400D HTRA2 His T7 Parkinson's L. Miguel Martins 16157 pET20b-delta133HtrA2 A141S HTRA2 His T7 Parkinson's...

  3. CRISPR Plasmids - Tagging

    Type
    Collection
    ...into human FBL locus Jorgensen Lab SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA... for improved efficiency and throughput over traditional tagging methods. Several different tagging techniques...CRISPR-Cas tagging system were tested by tagging transcription factors with FLAG in human cell lines. To repeat...insert genetic tags in the C. elegans genome. The SapTrap reaction produces a single plasmid targeting vector...vector that encodes both a guide RNA transcript and a repair template for an individual tagging event....26 plasmids; 21 of the plasmids are for use in SapTrap reactions for targeting vector assembly, and the...

  4. p53 Pathway

    Type
    Collection
    ... bind transcriptional co-activators and modulate transcription. The list of p53 transcriptional targets...collection of plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor activated by cellular...p53 Gene List References Background p53 is a transcription factor and tumor suppressor activated by cellular...mutations interfere with p53’s ability to activate transcription, and they also have a dominant negative effect...page are provided below. Symbol Name 14-3-3-σ Stratifin Apaf-1 Apoptotic peptidase activating factor 1... receptor superfamily, member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor...

  5. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... Dorus Gadella 58473 pEGFP-C1 F-tractin-EGFP Actin Filaments F-tractin EGFP Dyche Mullins 41147 pEGFP ...proteins from the cell to the extracellular environment. Proteins are first translated at the endoplasmic reticulum...remodeling the cytoskeleton or blocking membrane trafficking. Addgene has assembled a collection of fluorescent...Caveolae Cav1 mCherry Ari Helenius 37533 cfSGFP2-N Extracellular milieu cfSGFP2-N EGFP Ikuo Wada 73208 pcDNA3.1...2XG4S-mCer3-SKL Peroxisome SKL tripeptide, peroxisome transport signal dL5 FAP; mCerulean3 Marcel Bruchez 85065...reticulum, then are transported to the Golgi apparatus in vesicles to be packaged and shipped. Many of ...require chaperones to help with folding or post-translational modification to function properly. Browse or...

  6. COVID-19 Resources

    Type
    Collection
    ...(COVID-19) pandemic was caused by a novel virus strain from the Coronaviridae family called SARS-CoV-2...used for measuring viral entry and activity in neutralization assays and links to popular envelope and packaging...SARS-CoV-2-Human Interaction Network Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV...Anderson et al., 2020). BSG plasmids - (CD147), transmembrane glycoprotein of the immunoglobulin superfamily... protease (Mpro) activity reporters Reverse transcriptase, protease, and polymerase constructs for SARS-CoV...SARS-CoV-2 detection assays Neutralizing nanobody plasmids And more! ID Plasmid Description Industry PI...opens in a new window) Addgene's Protocol for RNA Extraction Without A Kit Bloom lab - Protocol and Reagents...

  7. Mammalian RNAi Tools

    Type
    Collection
    ... the double-stranded siRNA and degrades one of the strands. The remaining single-stranded siRNA then binds...silencing is achieved through the delivery of a double-stranded RNA (dsRNA) that matches the mRNA target sequence...short interfering RNA). Delivery can occur via transfection or viral delivery of a plasmid. shRNAs are often...vector is taken up by a host, the viral genome is transcribed, producing the shRNA. Dicer, an RNase III enzyme... mechanism, therapeutic targets, and delivery strategies for cancer therapy . International Journal of...

  8. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...incorporated into the DNA by reverse transcription. The edited DNA strand displaces the unedited 5’ flap and...replace” gene editing method in which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A...the target sequence where it nicks the non-target strand and generates a 3’ flap. The 3’ flap binds to the...sequence remains unchanged and available as a substrate for another round. Figure 1: Overview of prime...Moloney murine leukemia virus (M-MLV) reverse transcriptase. Engineering of the PE enzyme and related components...including exploring new sources for the reverse transcriptase. Many PE enzymes and related tools are available...

  9. AAV Viral Preps

    Type
    Collection
    ...Spagehetti Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation...and useful gene delivery tools that effectively transduce many mammalian cell types. AAV is used in a wide... Packaged on Request service. You can choose a transfer plasmid from our repository and request packaging...Ablation, CRISPR Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing Samples Small samples...Testing Panel for the Eye and Brain For enhanced transduction of the eye and other tissues All Serotypes If...

  10. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...may yield slightly different DNA concentrations. Can I directly transfect cloning grade DNA into cells? ...experiments by removing the amplification and extraction steps required when one receives plasmids in ...DNA Service Details Amount 2 µg Volume 20 µl Concentration 100 ng/µl (± 5 ng/µl) Buffer 10 mM Tris-Cl, ...validated by next generation sequencing. The DNA concentration and 260/280 ratio has been determined by spectrophotometer...designed for use in cloning applications. Direct transfection into cells is not recommended. Can I order more...

  11. CRISPR Plasmids and Resources

    Type
    Collection
    ...generate double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand DNA breaks. ...deletions. Insert NEW CRISPR transposases insert large fragments of DNA. Transcriptional Regulation Activate dCas9...Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information into a specific..., or fused to a repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier...

  12. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...124606 SOX2-mEGFP AICSDP-60 mEGFP Transcription factor SOX-2 Transcription Factor 124607 ACTN2-mEGFP AICSDP...Microtubules 101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase 1 Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP ...133963 UBTF-HaloTag AICSDP-80 HaloTag Nucleolar transcription factor UBF Nucleolus (fibrillar center) 133964...159745 TFAM-mEGFP AICSDP-72 mEGFP Mitochondrial transcription factor A (TFAM) Mitochondrial nucleoids 164499...human iPS cells (Link opens in a new window) RNP transfection for gene editing human iPS cells (Link opens...

  13. Neurodegeneration Research Collection

    Type
    Collection
    ...neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent rodent species...delivery of viral particles that will target the central nervous system, peripheral nervous system, vesicular...kinetics and localization for imaging synaptic transmission. Aggarwal et al. Nat Methods. 2023 May 4. See...Differentiation Factors Find plasmids used to transform iPSCs and fibroblasts into neurons and more. New...PiggyBac plasmids with tet-inducible expression of transcription factors for sensory, motor, and cortical neuron...Use a FRET-based method to monitor structural transitions in protein self-organization. Wan et al. Cell...

  14. Cancer Research Plasmids and Resources

    Type
    Collection
    ...mutations. Resources New Tool for Lineage Tracing: The ClonTracer Library (Frank Stegmeier) Tackling Cancers...kinase that anchors a signaling network for transitioning between anabolic and catabolic states. Ras Ras...frequently mutated gene in human cancer, is a transcription factor and tumor suppressor. Wnt Coming soon...cDNAs for studying oncogenic signaling pathways. ClonTracer Library : Barcoding library from the Stegmeier...

  15. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...21871 pMLM36 (aka pBR-UV5-GP-FD2) 21874 Strain KJ1C 21875 Strain CSH100 Content blocked, you may need to... Keith Joung This kit consists of plasmids and strains used to engineer ZFNs via the Oligomerized Pool... packaged plasmids and 3 individually packaged strains, which are shipped as bacterial stabs at room temperature... pAC-Kan-alphaGal4 13422 pBAC-lacZ 13424 KJBAC1 strain 21869 pKJ1267 (aka pAC-alphaGal4) 21870 pKJ1712...

  16. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...understood but are critical for developing therapeutic strategies to prevent viral spread. As a Biosafety Level...mechanisms of infection and to determine methods of neutralizing the virus or reducing its infective potential...that can be used for measuring infectivity and neutralizing activity are listed below: ID Plasmid Description...provirus and the overall strength of viral transcriptional response in these cells. Warner Greene 105621...Posts Viral Vectors 101: Pseudotyping Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV...

  17. Synthetic Biology - Overview

    Type
    Collection
    ...sensing. Strains Browse Addgene's collection of engineered bacterial strains, including plasmid-strain functional...Negative Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard...and pOSIP Plasmid Kit Gray Lab Maize and Rice Transcription Factors Hasty Lab Biopixels Jaramillo Lab Riboregulation...

  18. Distribution to Industry

    Type
    Collection
    ... Material Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement...agreement (MTA), including transfers to for-profit entities. The MTA is a legal agreement between the recipient... the depositing institution(s). It governs the transfer of biological materials and intellectual property...Please see the Sample Agreements on our Technology Transfer Information page for more information. To learn...

  19. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...in vitro transcription BsaI none S. pyogenes Mark pU6T7 71462 Mammalian/in vitro transcription BsaI none...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...yes, activate S. pyogenes Marraffini pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) 47868 Mammalian U6...toolbox for multiplexed plant genome editing and transcriptional regulation Plant Three types of plasmids available...recombination. Qi Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs Zebrafish Plasmids...multiple functional gRNAs from a single precursor transcript Bullock pCRISPathBrick Bacteria Allows rapid ...

  20. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...Addgene AAV transfer plasmids...or toxins should be handled at BSL-2. AAV can transduce both dividing and non-dividing cells with a low... AAV does not integrate into the host genome, transgene expression can be long-lived. The utility of AAV...effort directed toward expanding this capacity. Traditionally, AAV requires the presence of another "helper...viral genome (typically called cis -plasmid or transfer plasmid). Helper Plasmid ID Plasmid Serotype Description...
Showing: 121 - 140 of 1064 results