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We narrowed to 1,045 results for: fer

Showing: 141 - 160 of 1045 results

  2. AAV Molecular Tools

    Type
    Collection
    ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

  4. CRISPR Plasmids and Resources

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    ... activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor peptide, ...

  5. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

  6. Synthetic Biology - Overview

    Type
    Collection
    ...Negative Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard...

  8. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

  9. Brain Armamentarium

    Type
    Collection
    ...molecular genetic reagents to gain access to many different brain cell types. The expanded collection of Armamentarium...

  10. The Pleiades Promoter Project

    Type
    Collection
    ...pEMS1722 intron-lacZ/NLS Additional Resources References Portales-Casamar E, Swanson DJ, Liu L, de Leeuw...

  12. Genetic Code Expansion

    Type
    Collection
    ... directed evolution to charge the tRNA with a different amino acid. They are typically expressed from ...

  13. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Plasmids scFv Plasmids Nanobody Plasmids Protocols References The UC Davis/NIH NeuroMab Facility (Link opens...NeuroMab Protocols (Link opens in a new window) References Andrews NP, et al. 2019. A toolbox of IgG subclass-switched...

  14. Molecular Biology Reference

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    Guide
    ...Educational Resources Molecular Biology Reference Molecular Biology Reference Jump To: Molecular Biology Basics...contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of the genetic... C EDQVDPRLIDGK S Tag KETAAAKFERQHMDS SB1 PRPSNKRLQQ Webpage and Blog References Addgene's blog , including...Addgene's molecular biology reference guide, including information on molecular genetics, plasmids, sequencing...verification Resources Genetic Code Webpage and Blog References Protocols Origins of Molecular Genetics The concept... Joshua Lederberg coined the term plasmid, in reference to any extrachromosomal heritable determinant....bacterium to another by a type of horizontal gene transfer (conjugation), usually providing a benefit to ...

  15. Lentiviral Vector Guide

    Type
    Guide
    ...packaging genes. However, they are different viruses and require different isoforms of these packaging components...second-generation transfer plasmids as well. Third-generation plasmids include (Figure 4): Transfer plasmid — ...vectors separate transfer, envelope, and packaging components of the virus between different plasmids. The...activate the promoter on the transfer plasmid. Many of the lentiviral transfer plasmids that have been deposited...genes. From an experimental standpoint, the main difference between lentiviruses and gamma-retroviruses is...three (or four, for third generation) plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA ...systems, due to a wide range of infectivity for different cell types (known as tropism). Lentiviral vectors...

  16. Antibody Guide

    Type
    Guide
    ... dates. Buffers Buffers are a concern for conjugating and signaling molecules, as some buffer components...heavy chain constant region” or Fc. Different isotypes play different roles in the immune response, and ...removed from the buffer before the reaction. However, it can be added back to buffers after the conjugation...of colors activated by different wavelengths, allowing for easy differentiation between multiple antibodies... In some cases, it may be preferable to buy small aliquots of many different antibodies and do batch validation...an antigen, while the specific binding site is referred to as an epitope. Production Antibodies are typically...typically produced in one of three different methods: in animals, in cultured immune cells, or through...

  17. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... via a different plasmid. In order to produce rAAV vectors, three plasmids are needed: Transfer plasmid...s Molecular Biology Reference . Production The three production plasmids (transfer, packaging, and helper...2, and 5 display differential efficiency and cell tropism after delivery to different regions of the central...Integration AAV Types Common Uses Safety Resources and References Plasmid Elements Glossary Adeno-Associated Viruses...immune response. These features have made AAVs the preferred choice to be used as viral vectors for gene therapy...transgene, gRNA, or shRNA of interest into the transfer plasmid can be done with most standard cloning...restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction sites or...

  18. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...three different plasmids containing the transfer, envelope, or packaging components. The transfer plasmid...packaging genes. However, they are different viruses and require different isoforms of these packaging components...type. From an experimental standpoint, the main difference between lentiviruses and gamma-retroviruses is...gamma-retroviral vectors, you need three plasmids (Figure 2): Transfer plasmid — contains transgene, sgRNA, or shRNA ... gene, due to a wide range of infectivity for different cell types (known as tropism). Figure 2: Gamma-retroviral...transgene, gRNA, or shRNA of interest into the transfer plasmid can be done with most standard cloning...restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction sites or...

  19. Adenovirus Guide

    Type
    Guide
    ... (A to G). Different serotypes exhibit varying tissue tropisms, meaning they preferentially infect certain...from different species. Used to alter infectivity and tropism. pShuttle Class of shuttle/transfer vectors...Adenoviral Types Common Uses Safety Resources and References Plasmid Elements Glossary Adenoviruses (AdV) .... These genes are now supplied in trans , via different plasmids or the packaging cell line, which not...need two plasmids: Shuttle plasmid (also known as transfer plasmid) — containing the transgene of interest...recombination between the transfected adenoviral transfer plasmid and the E1 region integrated in the packaging...vectors Third-generation rAdV vectors are also referred to as "gutless" or high capacity AdV (HCAdV) vectors...

  20. CRISPR Guide

    Type
    Guide
    ...Alternatives Resources Plan Your Experiment Web References PAM Sequences Glossary Publications CRISPR Overview...techniques . Collectively, these techniques are referred to as CRISPR ( C lustered R egularly I nterspaced...measure of fidelity, so sometimes they will be referred to as increased fidelity enzymes. hfCas9s were...pyrimidine-to-pyrimidine) Base transversions - edit to different classification of nucleotide base (purine-to-pyrimidine...opposite strands Prime editing and base editing both offer high efficiency and low indel formation compared...genetic changes, including moving entire genes to different locations, by combining other gene editing mechanisms...DNA-targeting of CRISPR-Cas systems. You may also see them referred to as INTEGRATE ( I nsertion of T ransposable ...
Showing: 141 - 160 of 1045 results