We narrowed to 157 results for: itch
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TypeBlog Post...Trimmer, J. S. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced...
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Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog Post...27463678. 2. Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post... in to repair the damage. If all they can do is stitch the two broken ends together through non-homologous... -
Bacterial Expression Systems
TypeCollection... Photoactivatable , Photoconvertible , and Photoswitchable fluorescent proteins Various PIs Fluorescent... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection...B, Yao Z, Zhou T, Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith KA, Esposito L,... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...conversions are base transversions: they require switching between a two-ring purine and one-ring pyrimidine... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog Post...paired to each other? At this point, the protein can switch between the closed and open conformations to bend... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...type. While transdifferentiation is suited for switching cell types between similar lineages, reprogramming... -
Penn Vector Core Partnership with Addgene
TypeCollection...Function PI AV-1-49531P 100040-AAV1 pAAV.hSyn1.Twitch2B.WPRE.SV40 Biosensor Oliver Griesbeck AV-1-50942 50942... -
Delivery Methods for Generating iPSCs
TypeBlog Post...PubMed Central PMCID: 2677165. 3. Jia, Fangjun, Kitchener D. Wilson, Ning Sun, Deepak M. Gupta, Mei Huang... -
Deep Dive: qPCR
TypeBlog Post...may be easier to conceptualize. Just remember to switch them back before reporting the data! Statistical... -
Optogenetics Guide
TypeGuide...Kv) channels fused to LOV2 photoswitch. Illumination causes the photoswitch to unfold, lowering the toxin's...including explanations of microbial opsin and optical switch terminology. Science...Optogenetics Guide Introduction Microbial Opsins Optical Switches References Introduction The field of optogenetics...of proteins; e.g., microbial opsins and optical switches Sensors are genetically-encoded reporters of molecular...inhibitory neurons near the injection site. Optical Switches Researchers have harnessed plant and bacterial...protein systems controlled by light. These “photoswitchable” proteins offer exquisite spatial and temporal...through directed mutagenesis - use Addgene's Optical Switch Plasmid Table to search for plasmids for your next... -
Cloning
TypeGuide...Gibson Type IIS Ligation Independent Cloning Oligo Stitching Molecular cloning or the creation of recombinant...expensive. In addition it is quite difficult to switch to another cloning method, such as restriction ...Top Yeast-mediated Cloning and Oligonucleotide Stitching Yeast-mediated cloning is very similar in principle...advantage is the ability to perform oligonucleotide stitching, in which pieces of DNA that share no end homology... -
CRISPR Guide
TypeCollection...Davidson, A. R. (2016). Naturally occurring Off-Switches for CRISPR-Cas9. Cell , 167 (7), 1829-1838.e9.... -
Chemogenetics Guide
TypeGuide...receptor in unwanted cell types. Generating a FLEx switch to control expression of a chemogenetic reporter... -
Trimmer Lab NeuroMab Collection
TypeCollection...Andrews NP, et al. 2019. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced... -
CRISPR Guide
TypeGuide...Davidson, A. R. (2016). Naturally occurring Off-Switches for CRISPR-Cas9. Cell , 167 (7), 1829-1838.e9....