...Ligation Independent Cloning Yeast-Mediated & Oligo Stitching Resources Molecular cloning, or the creation of...expensive. In addition, it is quite difficult to switch to another cloning method, such as restriction ...BioRender.com. Yeast-mediated Cloning and Oligonucleotide Stitching Yeast-mediated cloning is very similar in principle...advantage is the ability to perform oligonucleotide stitching, in which pieces of DNA that share no homology...-mediated plasmid cloning and oligonucleotide stitching. Created with BioRender.com. Additional Resources...