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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 976 results for: nin

Showing: 141 - 160 of 976 results

  1. CRISPR Plasmids - Parasites

    Type
    Collection
    ...preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions...

  4. Plasmid Collections

    Type
    Collection
    ... view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology...

  5. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...lists both published and unpublished plasmids containing gRNA sequences designed to be used with any CRISPR...

  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...PRNP Dementia Susan Lindquist 22291 pcDNA3-FLAG-Synaptojanin 1-145 SYNJ1 Flag CMV Parkinsonism, early-onset...early-onset Pietro De Camilli 22292 pcDNA3-FLAG-Synaptojanin 1-170 SYNJ1 Flag CMV Parkinsonism, early-onset Pietro... Pietro De Camilli 22293 pEGFPC1-FLAG-Synaptojanin 1-145 SYNJ1 GFP, Flag CMV Parkinsonism, early-onset...early-onset Pietro De Camilli 22294 pEGFPC1-FLAG-Synaptojanin 1-170 SYNJ1 GFP, Flag CMV Parkinsonism, early-onset...

  7. AAV for Neuronal Tracing

    Type
    Collection
    ...from this cell to synaptically connected cells (meaning, it can spread to cells that are projecting to ...

  8. AAVED

    Type
    Collection
    .../factors that researchers should consider when planning experiments). What resources might be useful to...

  9. Arf GTPase Family

    Type
    Collection
    ...of GTPases is regulated both upstream (by GEFs, guanine nucleotide exchange factors), and downstream (by...

  10. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...EYFP connected by 1 flexible glycine- and serine-containing peptide linker (GGSGGS) repeat pET28CLY2 Peptide...

  13. Rett Syndrome

    Type
    Collection
    ...developmental delay between 6 and 18 months of age. A defining feature, with onset typically between 18 to 30...

  14. Retrograde AAV viral preps

    Type
    Collection
    ... plasmid with PCR, purity assessment by silver staining, in vitro and (when possible) in vivo testing,...

  15. Ligation Independent Cloning

    Type
    Protocol
    ... Protocols Ligation Independent Cloning Ligation Independent Cloning You may also ...Independent Cloning (LIC) obviates the need for the time-consuming ligation step of traditional cloning methods... Independent Cloning Protocols...also like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Overview Ligation Independent...methods. In traditional cloning, base-pairing in the short overlapping regions (usually 4 bp) does not provide... restriction sites from the final product (no "cloning scars"). A “stuffer” sequence allows for electrophoretic...design. Search Addgene's collection of empty LIC cloning vectors Protocol Step 1: Design Your Primers Primer...

  16. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...plasmid. Designing Primers for PCR Based Cloning The basic PCR primers for molecular cloning consist of... Protocols Plasmid Cloning by PCR Plasmid Cloning by PCR You may also...PCR based cloning carries a much higher risk for mutation than restriction enzyme based cloning. DNA replication...Protocols for plasmid cloning by PCR. Protocols...Ligation Bacterial Transformation Summary PCR based cloning is incredibly versatile and allows for nearly any...limitations. Background In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at...Restriction Site: Your chosen restriction site for cloning (usually 6-8bp). Hybridization Sequence: The region...

  17. Brain Initiative Collection

    Type
    Collection
    ...pAAV-CAG-iSeroSnFR-Nlgn Fluorescent reporter for serotonin (dendrite localization tag) Lin Tian 135420-AAV1...

  18. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ....1-TRC Cloning Vector A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids B. Designing shRNA...Digesting pLKO.1 TRC-Cloning Vector C.4 Ligating and transforming into bacteria D. Screening for Inserts D.1...pLKO.1 TRC-Cloning Vector I.2 Recipes I.3 Warranty information Back to Top A. pLKO.1-TRC Cloning Vector A...Figure 1 : Map of pLKO.1 containing an shRNA insert. The original pLKO.1-TRC cloning vector has a 1.9kb stuffer...vector containing non-hairpin insert. pLKO.1 – scramble shRNA Negative control vector containing scrambled...for “pLKO”“. Back to Top B. Designing shRNA Oligos for pLKO.1 B.1 Determining the Optimal 21-mer Targets...these bases are important for cloning the oligos into the pLKO.1 TRC-cloning vector. Forward oligo: 5’ CCGG...

  19. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type
    Protocol
    ...Changing a Multiple Cloning Site (MCS) Adding short tags - ex: HA or Flag Cloning shRNAs Background For... MfeI. With oligo overlap cloning, you can design a set of oligos containing your desired restriction ...using oligo overlap cloning. Protocols... Protocols Plasmid Modification by Annealed Oligo Cloning Plasmid Modification...Modification by Annealed Oligo Cloning You may also like... Restriction Digest of Plasmid DNA DNA Ligation Bacterial...Bacterial Transformation Summary Oligo overlap cloning can be used anytime you need to add a short stretch...digest of existing sites in the original vector. Designing oligos To add NdeI, PacI, AscI and MfeI sites ...

  20. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ... Protocols Subcloning Plasmid Cloning by Restriction Enzyme Digest (aka Subcloning) You may also like...Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental...out our Empty Backbone Reference . Background Subcloning by restriction digest is a commonly used lab ...element between plasmids. Let's assume that you are beginning a new project on your gene of interest (YGOI for...YGOI is in a bacterial expression vector. Using subcloning, you can easily move YGOI into a mammalian expression...your recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid...find two different restriction enzymes for your subcloning. It is also possible to use a single enzyme, ...
Showing: 141 - 160 of 976 results