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...insert in a 5,000bp backbone, but there are many plasmids out there that, when digested with restriction...restriction enzyme based technique for verifying plasmids like this is "plasmid fingerprinting", where you...

...efficient and effective method for the assembly of plasmids, and molecular biologists now use this method ... background and enrich for correctly assembled plasmids by designing primers to split an antibiotic resistance...

... it isn't methylation sensitive . Plasmids grown in Dam or Dcm methylase positive strains...activity . If you are digesting a large number of plasmids with the same enzyme(s) (for instance, in a diagnostic...

...h. Prepare a mixture of the three transfection plasmids into OptiPro SFM: Reagent Amount per 10 cm dish...removal step. For high quality plasmid DNA, the plasmids should also be propagated in an endonuclease negative...

...selectable markers, or any other DNA element between plasmids. Let's assume that you are beginning a new project...restriction digests for your donor and recipient plasmids. Because you lose some DNA during the gel purification...

... a new one. When developing the assay multiple plasmids containing ITR were tested. Plasmid #59462 is ... Use the recommended plasmid, or test multiple plasmids to find a suitable one. Some labs have reported...

...protocol, but their conditions must be optimized. Plasmids for transfection: pHelper; pRC (Rep-Cap), plasmid... the total number of base pairs from all three plasmids, we can determine the total μg/bp we need to achieve...

...high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for use in western blot, immunoprecipitation... (tagged or untagged) protein When expression plasmids are available, or the necessary expertise to clone...involves the transient transfection of expression plasmids in a given cell line. For flow cytometry, it is...

...as ethanol. Writing on the tops of the tubes and lids of items to be placed in the water bath is often...

...contaminants with lower densities, including empty capsids. The 60% step serves as a cushion for genome-containing...

... (A) Cumulative CRISPR plasmids shipped by year. (B) Cumulative CRISPR plasmids deposited with Addgene...,000 CRISPR plasmids in our repository, and we have shared almost 300,000 CRISPR plasmids and 12,000 CRISPR...these CRISPR plasmids (and libraries)? We wanted to know, so we classified CRISPR plasmids containing a...CRISPR plasmids requested each year by year of deposit (Figure 3), we saw that once CRISPR plasmids become... CRISPR plasmids seem to never truly go out of style. Instead, an almost even mix of plasmids (by age)...Total CRISPR plasmids deposited by country; square size is proportional to number of plasmids deposited ...Total CRISPR plasmids requested by country; square size is proportional to the number of plasmids requested...

...rectangles) are cloned into various plasmids using oligonucleotides. These plasmids contain Type IIS restriction...Golden Gate/Gibson Assembly Multiplexing Plasmids: These plasmids allow you to assemble 2-4 gRNAs through...Golden Gate/Gibson Assembly Multiplexing Plasmids: These plasmids can be used to express up to 8 gRNAs after...Yang Lab Single Transcript Multiplexing Plasmids: These plasmids are similar to the Csy4 polycistronic ...Chen Lab Golden Gate Assembly Multiplex Plasmids: These plasmids allow expression of 2-5 gRNAs in zebrafish...tools to help you multiplex - we’ll use mammalian plasmids to introduce you to some of your potential options...and cloning methods, but please scroll down for plasmids suitable for other model systems, including E....

...received over 13,000 plasmids requests for COVID-19 related plasmids. These plasmids have reached labs in...small collection of plasmids for coronavirus research, including a handful of plasmids that have been in...some highlights of the plasmids shared over the last year and how these plasmids have been used to accelerate...circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited from... labs also deposited plasmids for producing COVID-19 diagnostic enzymes. Plasmids in this toolkit include...These plasmids contain both untagged and tagged versions of SARS-CoV-2 ORFs and include plasmids that ... has now grown into a collection of over 2,400 plasmids, with many available to scientists in industry...

...sequencing all incoming plasmids. If you are interested in requesting one of these plasmids, you can request ...data for each of their plasmids, from descriptions of key components of the plasmids to partial, full, or...the identified differences, the plasmids are sent out to Plasmidsaurus for long-read nanopore sequencing...amplification and manipulation to produce and edit plasmids. These are prone to error — both human and biological...especially shared ones.  The importance of QC Plasmids are a fundamental part of research. They are easy...cloning process — including the propagation of plasmids in bacteria — has many points at which small errors...what was originally intended. Sharing unverified plasmids can lead to far reaching consequences, from wasting...

...associated plasmids with Addgene. Since then, Addgene has filled over 6,500 requests for these plasmids. While...those containing plasmids that the authors deposited with Addgene and those without plasmids deposited with...2,596 of which had plasmids deposited with Addgene. Articles with Addgene plasmids get more citations ...with plasmids deposited at Addgene – typically about four times as many as papers without plasmids deposited...Papers without Addgene Plasmids in Journal (E) Percent of Papers with Addgene Plasmids in Journal (F) Weight...get cited more?   Articles with and without plasmids at Addgene To find out whether scientific articles...journals from 2005 to 2015 in areas most relevant to plasmids: Genetics & Heredity, Cell Biology, Biotechnology...

...inducible inactivation. Find the Robinson plasmids or the Royle plasmids and get to work! Robinson et al...mGRASP plasmids. Additionally, the lab recently described the use of another set of mGRASP plasmids in their... know what plasmids you would like to seen on our Hot Plasmid list. Other popular plasmids & blog posts...sgRNA libraries expand upon the CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA...new CRISPR screening tools, see our CRISPR/Cas Plasmids: Pooled Libraries webpage or read our blog post...resources at www.addgene.org/CRISPR/ to browse CRISPR plasmids, watch informational videos, download protocols... with extremely-dim brightness, bfloGFPc1. The plasmids used for determining the structures are now available...

...contains all the plasmids from the same lab while the publication page contains all the plasmids deposited from...as ccdB survival (for Gateway plasmids).  We also like to store plasmids that need extra stability, such...construct by the depositing lab. There may be other plasmids with similar names in the collection, but you ...The flame that appears beside some of Addgene’s plasmids is an indication of popularity. A yellow flame...blue, more than 100 times! When you search for plasmids on our website, one of the available filters popularity... popularity (e.g. only showing those plasmids which have been ordered more than 100 times).     I guess... pages is relatively new, so some of our older plasmids may not have an entry in this field. If you’ve...

...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase...deposited a pair of plasmids for the cotranslational incorporation of unnatural amino acids (UAAs) into proteins...reactions, fluorescent amino acids, posttranslationally modified amino acids, photoaffinity probes, and ... can visualize: Cell Structure Plasmids: Cell Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61... our community of depositors. With over 40,000 plasmids avaliable in the repository, we can't give all...mediated genome modification and gene expression. The plasmids associated with this article can be obtained through...Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible...

...All plasmids deposited at Addgene enhance scientific sharing worldwide but a select few are particularly...particularly special. Over 1000 of our 60,000+ deposited plasmids have been requested more than 100 times each, ...to sharing epitomized by the creators of these plasmids with the first annual Blue Flame Awards. What'...scientists around the world choose to share their plasmids through our repository since we know biological...Addgene has transformed the way in which we share plasmids and other molecular tools with the scientific ...throughout the research community. These popular plasmids are categorized into a 3-level system on our website...depositing PI’s who have the most highly requested plasmids. Currently, there are roughbly 400 depositors ...

...transfecting four different plasmids into the producer cells (two packaging plasmids, an envelope plasmid, ...transfer plasmids replication competent? What is SIN? A6: Most (if not all) Addgene transfer plasmids are ...Addgene's most popular lentiviral plasmids Overview of Lentiviral Packaging Plasmids Lentiviral Protocols and ...Addgene has an extensive collection of lentiviral plasmids created for a variety of applications including...I package lentivirus with retroviral packaging plasmids (and vice versa)? A1: Lentiviruses are part of...actually work? That depends. The majority of transfer plasmids use the weak viral LTR promoter to drive expression...available here). 2nd vs. 3rd Generation Lentiviral Plasmids Q3: How can you tell 2nd- and 3rd-generation transfer...