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Showing: 161 - 180 of 878 results

  2. Lentivirus Production

    Type
    Protocol
    ...h. Prepare a mixture of the three transfection plasmids into OptiPro SFM: Reagent Amount per 10 cm dish...removal step. For high quality plasmid DNA, the plasmids should also be propagated in an endonuclease negative...

  3. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...selectable markers, or any other DNA element between plasmids. Let's assume that you are beginning a new project...restriction digests for your donor and recipient plasmids. Because you lose some DNA during the gel purification...

  4. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ... a new one. When developing the assay multiple plasmids containing ITR were tested. Plasmid #59462 is ... Use the recommended plasmid, or test multiple plasmids to find a suitable one. Some labs have reported...

  5. AAV Production in HEK293 Cells

    Type
    Protocol
    ...protocol, but their conditions must be optimized. Plasmids for transfection: pHelper; pRC (Rep-Cap), plasmid... the total number of base pairs from all three plasmids, we can determine the total μg/bp we need to achieve...

  6. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for use in western blot, immunoprecipitation... (tagged or untagged) protein When expression plasmids are available, or the necessary expertise to clone...involves the transient transfection of expression plasmids in a given cell line. For flow cytometry, it is...

  7. Water Bath Protocol

    Type
    Protocol
    ...as ethanol. Writing on the tops of the tubes and lids of items to be placed in the water bath is often...

  9. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ... (A) Cumulative CRISPR plasmids shipped by year. (B) Cumulative CRISPR plasmids deposited with Addgene...,000 CRISPR plasmids in our repository, and we have shared almost 300,000 CRISPR plasmids and 12,000 CRISPR...these CRISPR plasmids (and libraries)? We wanted to know, so we classified CRISPR plasmids containing a...CRISPR plasmids requested each year by year of deposit (Figure 3), we saw that once CRISPR plasmids become... CRISPR plasmids seem to never truly go out of style. Instead, an almost even mix of plasmids (by age)...Total CRISPR plasmids deposited by country; square size is proportional to number of plasmids deposited ...Total CRISPR plasmids requested by country; square size is proportional to the number of plasmids requested...

  10. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...rectangles) are cloned into various plasmids using oligonucleotides. These plasmids contain Type IIS restriction...Golden Gate/Gibson Assembly Multiplexing Plasmids: These plasmids allow you to assemble 2-4 gRNAs through...Golden Gate/Gibson Assembly Multiplexing Plasmids: These plasmids can be used to express up to 8 gRNAs after...Yang Lab Single Transcript Multiplexing Plasmids: These plasmids are similar to the Csy4 polycistronic ...Chen Lab Golden Gate Assembly Multiplex Plasmids: These plasmids allow expression of 2-5 gRNAs in zebrafish...tools to help you multiplex - we’ll use mammalian plasmids to introduce you to some of your potential options...and cloning methods, but please scroll down for plasmids suitable for other model systems, including E....

  11. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...received over 13,000 plasmids requests for COVID-19 related plasmids. These plasmids have reached labs in...small collection of plasmids for coronavirus research, including a handful of plasmids that have been in...some highlights of the plasmids shared over the last year and how these plasmids have been used to accelerate...circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited from... labs also deposited plasmids for producing COVID-19 diagnostic enzymes. Plasmids in this toolkit include...These plasmids contain both untagged and tagged versions of SARS-CoV-2 ORFs and include plasmids that ... has now grown into a collection of over 2,400 plasmids, with many available to scientists in industry...

  12. A Look at Addgene's QC Process

    Type
    Blog Post
    ...sequencing all incoming plasmids. If you are interested in requesting one of these plasmids, you can request ...data for each of their plasmids, from descriptions of key components of the plasmids to partial, full, or...the identified differences, the plasmids are sent out to Plasmidsaurus for long-read nanopore sequencing...amplification and manipulation to produce and edit plasmids. These are prone to error — both human and biological...especially shared ones.  The importance of QC Plasmids are a fundamental part of research. They are easy...cloning process — including the propagation of plasmids in bacteria — has many points at which small errors...what was originally intended. Sharing unverified plasmids can lead to far reaching consequences, from wasting...

  13. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...associated plasmids with Addgene. Since then, Addgene has filled over 6,500 requests for these plasmids. While...those containing plasmids that the authors deposited with Addgene and those without plasmids deposited with...2,596 of which had plasmids deposited with Addgene. Articles with Addgene plasmids get more citations ...with plasmids deposited at Addgene – typically about four times as many as papers without plasmids deposited...Papers without Addgene Plasmids in Journal (E) Percent of Papers with Addgene Plasmids in Journal (F) Weight...get cited more?   Articles with and without plasmids at Addgene To find out whether scientific articles...journals from 2005 to 2015 in areas most relevant to plasmids: Genetics & Heredity, Cell Biology, Biotechnology...

  14. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...inducible inactivation. Find the Robinson plasmids or the Royle plasmids and get to work! Robinson et al...mGRASP plasmids. Additionally, the lab recently described the use of another set of mGRASP plasmids in their... know what plasmids you would like to seen on our Hot Plasmid list. Other popular plasmids & blog posts...sgRNA libraries expand upon the CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA...new CRISPR screening tools, see our CRISPR/Cas Plasmids: Pooled Libraries webpage or read our blog post...resources at www.addgene.org/CRISPR/ to browse CRISPR plasmids, watch informational videos, download protocols... with extremely-dim brightness, bfloGFPc1. The plasmids used for determining the structures are now available...

  15. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...contains all the plasmids from the same lab while the publication page contains all the plasmids deposited from...as ccdB survival (for Gateway plasmids).  We also like to store plasmids that need extra stability, such...construct by the depositing lab. There may be other plasmids with similar names in the collection, but you ...The flame that appears beside some of Addgene’s plasmids is an indication of popularity. A yellow flame...blue, more than 100 times! When you search for plasmids on our website, one of the available filters popularity... popularity (e.g. only showing those plasmids which have been ordered more than 100 times).     I guess... pages is relatively new, so some of our older plasmids may not have an entry in this field. If you’ve...

  16. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase...deposited a pair of plasmids for the cotranslational incorporation of unnatural amino acids (UAAs) into proteins...reactions, fluorescent amino acids, posttranslationally modified amino acids, photoaffinity probes, and ... can visualize: Cell Structure Plasmids: Cell Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61... our community of depositors. With over 40,000 plasmids avaliable in the repository, we can't give all...mediated genome modification and gene expression. The plasmids associated with this article can be obtained through...Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible...

  17. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    ...All plasmids deposited at Addgene enhance scientific sharing worldwide but a select few are particularly...particularly special. Over 1000 of our 60,000+ deposited plasmids have been requested more than 100 times each, ...to sharing epitomized by the creators of these plasmids with the first annual Blue Flame Awards. What'...scientists around the world choose to share their plasmids through our repository since we know biological...Addgene has transformed the way in which we share plasmids and other molecular tools with the scientific ...throughout the research community. These popular plasmids are categorized into a 3-level system on our website...depositing PI’s who have the most highly requested plasmids. Currently, there are roughbly 400 depositors ...

  18. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...transfecting four different plasmids into the producer cells (two packaging plasmids, an envelope plasmid, ...transfer plasmids replication competent? What is SIN? A6: Most (if not all) Addgene transfer plasmids are ...Addgene's most popular lentiviral plasmids Overview of Lentiviral Packaging Plasmids Lentiviral Protocols and ...Addgene has an extensive collection of lentiviral plasmids created for a variety of applications including...I package lentivirus with retroviral packaging plasmids (and vice versa)? A1: Lentiviruses are part of...actually work? That depends. The majority of transfer plasmids use the weak viral LTR promoter to drive expression...available here). 2nd vs. 3rd Generation Lentiviral Plasmids Q3: How can you tell 2nd- and 3rd-generation transfer...

  19. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    ...cloning vector. Lentiviral Plasmids -  If you're familiar with the pLKO plasmids you'll note that those are...Actually, these two plasmids from Didier Trono's lab are our two most popular plasmids of the past 10 years...influx of plasmids for reprogramming differentiated cells into pluripotent stem cells. Plasmids from Shinya... have 19 different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene ...with all the new plasmids. So far, we have distributed over 10,000 CRISPR/Cas9 plasmids to more than 3,...time, Addgene has shipped over 350,000 individual plasmids to 5,000 different research institutions. This...2009 was ubiquitin. The most requested ubiquitin plasmids were pRK5-HA-Ubiquitin-WT and HA-Ubiquitin, and...

  20. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ...organizations have made their plasmids available beyond academia. For example: Plasmids from the Michael J. Fox...and plasmids from several labs at Florida State University became openly available. Find plasmids available...one critical part was missing when the COVID-19 plasmids first became available: distribution was limited...universities to make COVID-19 research-related plasmids available more broadly: to scientists in academia...unprecedented amount of support about allowing use of plasmids for research by anyone. As infection rates were...scientific community speed research is to offer plasmids created as part of research projects, another ...up with Ginkgo Bioworks to quickly bring these plasmids to the scientific community - both academic and...
Showing: 161 - 180 of 878 results