...our Search page to look for specific genes or applications that are not listed here. Addgene Microbiology...Densmore Lab EcoFlex MoClo : Modular cloning for applications like recombinant protein purification and cell-free...fluorescent protein plasmid collection is organized by application and by color. External Resources European Saccharomyces...

... the human brain through the development and application of innovative tools enabling large-scale real-time.... Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting 164450-PHPeB CN1851-rAAV-hI56i-minBglobin-iCre...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Additional Resources To learn more, please...

...your dissertation. Well, that will probably never happen. Yet with transcription activator-like (TAL) effectors... they still have potential targetable sites approximately every 35bp. Researchers are still determining...arrays from 12-31 repeats. Perhaps one of the most appealing features of the TAL effector arrays is the ease...

...There are approximately 2-4 million proteins per cubic micron in bacteria, yeast, and mammalian cells...based affinity purification and mass spectrometry approaches. Recently however, scientists have developed ...miniturbo Split-BioID: A conditional proteomics approach to monitor protein-protein interactions Split-... PMC3308701. Rhee, Hyun-Woo, et al. "Proteomic mapping of mitochondria in living cells via spatially restricted..., et al. "Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally...proximity-based labeling methods for interactome mapping. F1000Research, 8.  ...

...occasionally, using tetracycline off or tTA is more appropriate. If your gene of interest should be mostly inactive...occasionally, using tetracycline on or rtTA is more appropriate. Basic components Components for a tetracycline...Lentiviral, miRNA expression (PRIME) system for application in knockdown of gene expression at a single copy...

... antibody escape mutants and for comprehensive mapping. Some of the following plasmids are available to... Spike protein RBD mutations for comprehensive mapping (Jesse Bloom). SARS-CoV-2 Spike Receptor Binding... Spike protein RBD mutations for comprehensive mapping (Jesse Bloom, Tyler Starr). External Resources ...

...can run multiple apps at a time and can install new apps from a constantly growing app collection. It is... early smart phone with only one word processing app—revolutionary and the first of its kind but lacking... like a smartphone that can now run several more apps. The expanded functions of this “CRISPR 2.0” include...care for some Mendelian diseases.  The early applications of CRISPR/Cas9 Nonetheless, as powerful as CRISPR-Cas9...lab and as promising as it may be for clinical applications, it was initially limited to a couple of functions...addgene.org); at least one sgRNA-PBS (single-guide RNAs appended to one or more copies of Pumilio binding sites...pAC1392     pAC1404 pAC1411       Example applications of Casilio Using Casilio it is possible to recruit...

... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin...

...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control by Addgene...Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other antibodies...

...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control by Addgene...Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other antibodies...

...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Use this guide to learn more about the many applications of fluorescent proteins (FPs) and to find the...

...upstream of a 5' NGG 3' PAM sequence). Which CRISPR application is this gRNA sequence compatible with? CRISPR...Resources page have been used to indicate the Cas9 application the gRNA was designed to accomplish. Validated...Gene Target Species Target Sequence Plasmid ID Application Cas9 Species PubMed ID Depositor OCT4 H. sapiens...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...upstream of a 5' NGG 3' PAM sequence. Which CRISPR application is this gRNA sequence compatible with? CRISPR...

...family of transporters in the human genome with approximately 450 identified members. Many SLCs have mutations... benefit of basic academic research as well as applied research in small biotech and pharmaceutical companies...

...development the lab utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate gene targets...standard desalted oligonucleotides with the appropriate overhangs: The plasmids listed here are provided...

...single round of viral entry and infection. This approach allows researchers to safely study mechanisms ...for SARS-CoV-2 and other coronaviruses using appropriate packaging plasmids. Use the search bar to find...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...and are thus well suited to directed evolution applications. Examples of these base editing systems include...

...cells and will interfere with conjugation. For applications such as these, the buffer can be removed by ...tissue panel in one of the antibody’s recommended applications. The specific QC experiments performed varies...

... Editing in Caenorhabditis elegans. A modified approach has also been developed by the Fire laboratory...Other Resources Addgene Blog Posts An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency Fluorescent...

... any application Balanced Salt Solution (BSS) An ophthalmic solution suitable for any application TMN200...