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Showing: 41 - 60 of 511 results

  1. Control AAV Preps

    Type
    Collection
    ...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Please...

  2. Church Lab CRISPR Plasmids

    Type
    Collection
    ...with custom guide RNA (gRNA) in human cells: this involves co- expression of a Cas9 protein bearing a C terminus...

  3. Viral Production

    Type
    Collection
    ...correct integrated provirus using PCR. This assay involves transducing cells with serial dilutions of the...

  4. Chemogenetics Plasmids

    Type
    Collection
    ...chemogenetic resources available at Addgene. See our inventory of chemogenetic constructs, including DREADD constructs...

  6. Tetracycline Inducible Expression

    Type
    Collection
    ...which can result in unintended system induction. Investigate whether or not your serum is tetracycline-free...

  8. Optogenetics AAV Preps

    Type
    Collection
    ...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Please...

  10. Bacterial Expression Systems

    Type
    Collection
    ...of interest. pJT106b3 31393 Red light inducible inverter Red light (650 nm) Christopher Voigt When combined...

  11. Biosensor AAV Preps

    Type
    Collection
    ...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Please...

  12. Luciferase Plasmids

    Type
    Collection
    ...Luciferase-containing plasmids are commonly used to investigate the effect of regulatory elements, such as promoters...

  15. Fluorescence Titering Assay

    Type
    Protocol
    ...incubator Pipet controller Hazardous waste container Inverted fluorescence microscope Reagents DMEM high glucose...mL of DMEM complete. Mix well by pipetting or inverting. Aliquot 2 mL of cell suspension into each well...

  16. Kit Free RNA Extraction

    Type
    Protocol
    ... thoroughly by inversion. Add 1 mL water-saturated phenol, mix thoroughly by inversion. Add 0.2 mL of ...

  17. Antibody Guide

    Type
    Collection
    ...fluorophores used is important. It is best to inversely pair the brightness of the fluorophore with the...
Showing: 41 - 60 of 511 results