We narrowed to 183 results for: mec 1 cells

... J. 2010 Dec 1. Wolf Frommer ATP (extracellular) ecAT3.10 extracellular ATP sensor (cell surface targeted...SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation. Cell Rep. 2019 Jun 25;27(13):...Islands of Cells for multimodal recordings of cellular physiology. Nat Commun. 2020 Aug 4;11(1):3881. Adam...imaging-guided cell selection for evolving genetically encoded fluorescent indicators. Cell Rep Methods....biosensor for visualization of acetyl-CoA in live cells. Cell Chem Biol. 2025 Jan 21: S2451-9456(25)00002-9... Tantama ATP QUEEN ATP sensors for live-cell imaging Live cell imaging of metabolic heterogeneity by quantitative...

...Laplante M, Sabatini DM. Cell. 2012 Apr 13;149(2):274-93. doi: 10.1016/j.cell.2012.03.017. PubMed PMID... (mTOR) is a key metabolic regulator controlling cell growth and proliferation. mTOR, a serine/threonine...signals to determine the nutritional state of the cell and regulates the switch from anabolic to catabolic...growth factors, including insulin. mTORC2 influences cell survival and cytoskeletal organization. Cancer Impact... promotes the conditions needed for uncontrolled cell growth and proliferation, including an abundant ...appears to signal upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids...threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 PRAS40 Also known as AKT1S1...

...receptor superfamily, member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor Gadd45 ...-phase expressed 1; also known as GTSE1 BAI-1 Brain-specific angiogenesis inhibitor 1 Bax BCL2-associated...inhibitor type 1), member 1 PERP TP53 apoptosis effector PIDD p53-induced death domain protein 1 PIGs Etoposide...p53 are in promoting cell cycle arrest, apoptosis, or senescence in damaged cells. The p53 name arose ... Name 14-3-3-σ Stratifin Apaf-1 Apoptotic peptidase activating factor 1 ATM ATM serine/threonine kinase...kinase 4 or 6 CHK1 Checkpoint kinase 1 CHK2 Checkpoint kinase 2 Cop-1 Ring finger and WD repeat domain 2...SESN1 SESN2 SESN3 Sestrins 1, 2, or 3 Siah Siah E3 ubiquitin protein ligase 1 TSC2 Tuberous sclerosis 2...

...IGHDY1 IGHD1-1 immunoglobulin heavy diversity 1-1 IGHD11 IGHD1-14 immunoglobulin heavy diversity 1-14 (non-...IGHV6-1 immunoglobulin heavy variable 6-1 IGHV61, VH IGHV7-4-1 immunoglobulin heavy variable 7-4-1 IGHV7...foreign antigens to adaptive immune cells, such as T cells and B cells. About a week following the initial...presenting cells. B cells in secondary lymphoid organs recognize specific antigens through the B cell receptor...have been described. NK Cell Signaling Natural killer (NK) cells can kill cells that have been infected... within an infected cell membrane, triggering cell death. TCR Signaling The T cell receptor (TCR) engages...through effects on numerous immune cell types including T cells and B cells. It also impacts the development...

..., complex 1 SAV1 Salvador family WW domain containing protein 1 SCRIB Scribbled planar cell polarity protein... of kappa light polypeptide gene enhancer in B-cells 1 PAK PAK1 PAK2 PAK3 PAK4 p21 protein (Cdc42/Rac)...TANK binding kinase 1 TFDP TFDP1 TFDP2 Transcription factor Dp TIAM TIAM1 TIAM2 T-cell lymphoma invasion... dependent protein kinase 1 PEBP1 Phosphatidylethanolamine binding protein 1 PIK3 Catalytic Subunits PIK3CA...isomerase, NIMA-interacting 1 PLXNB1 Plexin B1 PPP1CA Protein phosphatase 1 catalytic subunit alpha PREX2...family member RB1 Retinoblastoma 1 RCE1 Ras converting CAAX endopeptidase 1 RHEB Ras homolog enriched in ...guanine nucleotide exchange factor 1 YAP1 Yes associated protein 1 Return to top Resources RAS Pathway...

...with Haploid Embryonic Stem Cells Carrying a Guide RNA Library. Cell Stem Cell . 17 (2), 221–232. PMID: ...in the same cell. This is called multiplexing , and increases the likelihood that any cell containing ...can increase efficiency. Synchronizing cells or modulating the cell cycle can help as HDR takes place during...expressing stable cell line. After applying your screening conditions to your transduced cell population, you...tracking of RNA in live cells, including orthogonal RNA/DNA labeling in the same cell. Figure 11: Overview...promiscuity is unique to bacterial cells and does not occur in mammalian cells. SHERLOCK , developed by the ...genomic loci in living human cells by an optimized CRISPR/CAS system. Cell . 155 (7), 1479–1491. PMID: ...

...genomic loci in living human cells by an optimized CRISPR/Cas system. Cell . 155(7):1479-91. PMID: 24360272... for Mapping Multiprotein Complexes in Human Cells. Cell Rep . 13(3):621-33. PMID: 26456817 Fu Y, Sander... cleaves it to destroy the invader ( Figure 1 ). Figure 1: An overview of the endogenous Type II bacterial...described CRISPR-based genome editing in mammalian cell culture. Five years later, PubMed lists more than...multiple loci can be easily expressed in the same cell or organism. The ability to precisely target CRISPR...genome edits or double-strand breaks. In mammalian cells, Cas13-ADAR2 does not require a specific flanking...proteins can be used to image genomic loci in live cells. RNA targeting : Cas13 enzymes target RNA rather...

...genome editing in mammalian cells (Cong et al ., Science 2013, Ran et al ., Cell 2013, Ran et al ., Nature...Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6...Matoba S, Zhang Y, Zhang F. Cell . 2013 Sep 12;154(6):1380-9. doi: 10.1016/j.cell.2013.08.021. Epub 2013 Aug...Feng G, Sharp PA, Zhang F. Cell . 2014 Oct 9;159(2):440-55. doi: 10.1016/j.cell.2014.09.014. Epub 2014 Sep...CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease...cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return to top AAV - Cas9 mouse CRISPR-Cas9...constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice the CRISPR-Cas9 system can ...

...Sontheimer, E. J. (2009). Origins and mechanisms of miRNAs and siRNAs . Cell, 136 (4), 642–655. PubMed (Link... in therapeutic settings. As a tool in mammalian cell systems, silencing is achieved through the delivery... design and delivery, see resources below. Figure 1: Overview of shRNA-mediated RNA interference. Created...Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol Other Addgene viral protocols...interference (RNAi) is an RNA-mediated gene silencing mechanism. It is a useful tool to investigate the roles ...the target. RNAi using siRNA follows a similar mechanism. Since it is pre-processed, it can bypass the ...Abadi, S. J., & Gharagouzlo, E. (2017). Molecular mechanisms and biological functions of siRNA . International...

...Unpublished Joost Gribnau Human Cell Line Models Induced pluripotent stem cells (iPSCs) are a powerful system...opens in a new window) LUHMES cell line , an immortalized pre-neuronal cell line that can be differentiated... RSRT cell line collection (186.8 KB) . To order, please email [email protected] The following cell lines ...information Mouse and Cell Line Repositories (Link opens in a new window) Coriell Institute - cell line repository...approximately half of cells expressing wild-type MECP2 and half expressing mutant MECP2 . Skewing of these...Plasmid Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR Tools Plasmids Resources... treat Rett syndrome and restore functional MECP2 in cells. Search (Link opens in a new window) JAX Mice...

...be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the Class 1 family of CRISPR systems...individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse...bacteria and archaea. While abundant in nature, Class 1 systems are largely underutilized compared to their...

...plasmids . Figure 1: Tet-regulated expression systems. Left: natural TetR mechanism; center: Tet-Off; ...expression of the transactivator proteins in mammalian cells or other species by codon optimization of their ...gene of interest from two separate vectors. Stable cell lines or transgenic animals can be made to achieve... and ensures that all transfected or transduced cells express both components in the same relative ratio...derivatives can be found in Krueger et al., 2004. Some cell culture sera may contain tetracycline or its derivatives...expression of mouse Oct4, Sox2, Klf4, and Myc for iPS cell generation Rudolf Jaenisch 51543 FUW-tetO-hOKMS ...expression of human Oct4, Sox2, Klf4, and Myc for iPS cell generation Tarjei Mikkelsen 172115 PB-TO-hNGN2 Piggybac...

...ECFP EYFP 434 0.41 527 67,000 0.67 4.8 1.5 pmECFP-1 , pmEYFP-1 , pECFP18aaEYFP mCerulean mVenus 433 0.49...nm), ε A : Acceptor exctinction coefficient (M -1 cm -1 ), QY A : Acceptor quantum yield, R 0 : Förster...emission and acceptor excitation spectra (×10 15 M -1 cm -1 nm 4 ). Values obtained from FPbase.org FRET calculator...influenced by many factors, such as the organism or cell type, relative position and orientation of the fluorophores... (Unpublished) FRET Indicators - Live and Fixed Cells Additional Resources at Addgene Learn more on the... the donor lifetime. FRET is often used to study (1) protein-protein interactions where each protein is...

...then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single...firefly luciferase to simultaneously measure the # of cells containing reactivated latent provirus and the overall...strength of viral transcriptional response in these cells. Warner Greene 105621 Lenti-luciferase-P2A-Neo Lentiviral... find a particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description...Spike Plasmids Accessory Plasmids Other Resources Mechanisms of SARS-CoV-2 infection remain poorly understood...This approach allows researchers to safely study mechanisms of infection and to determine methods of neutralizing...

...190293 Anti-AMIGO-1 [L86/14] AMIGO-1 Mouse Mouse IgG2a 190294 Anti-AMIGO-1 [L86/2] AMIGO-1 Mouse Mouse IgG2a...ATPase 1 [L60/4R-2b] Copper ATPase 1 Human Mouse IgG2b 206648 Anti-AMIGO-1 [L86/33R-2b] AMIGO-1 Mouse ...channel [L6/60R-1] Slo1 K+ channel Mouse Mouse IgG1 206695 Anti-AMIGO-1 [L86/33R-1] AMIGO-1 Mouse Mouse IgG2a...Anti-Olig1 [N149/25R-1] Olig1 Mouse Mouse IgG1 206702 Anti-Neurexin-1-Beta [N170A/1R-1] Neurexin-1-Beta Human Mouse...222160 Beclin-1 [N248A/15R] Beclin-1 Mouse Mouse IgG2a 222161 Beclin-1 [N248A/22R] Beclin-1 Mouse Mouse ...Mouse Mouse 190534 Neurexin-1-Beta scFv [N170A/1] N170A/1 scFv Neurexin-1-Beta Human Mouse 190535 PSD-...Mouse 206755 IP3 receptor, type 1 scFv [L24/1] L24/1 scFv IP3 receptor, type 1 Rat Mouse 206756 VSP scFv [...

...preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes...PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair ...occurs at a much lower efficiency than NHEJ. Figure 1: Overview of NHEJ and HDR following cleavage by Cas9...

...enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs from deleting both...gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids 159654... make dual guide expressing plasmids dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence.... Plasmids 175573 and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express... pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 ( Addgene #110821 ) VPR ( Addgene #63798) Plasmids...

...unique recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration...highly conserved proteoglycans crucial for regulating cell signaling during development. The IPI collection...and interrogate with conventional reagents. Figure 1: Axon guidance cues and receptors regulate the development... top Integrins Integrins are a family of mechanotransduction receptors, comprised of alpha-beta subunit...antibodies that target epitope tags and other common cellular proteins. ID Recombinant Antibody Description ...

...protein-protein interactions and nerve cell function within the cell. Parkinson's Disease Parkinson’s disease...endogenous gene tagging in human cells from the Allen Institute for Cell Science Collection . Express EGFP-tagged...that causes the progressive degeneration of nerve cells in the brain. Symptoms include changes in behavior...siRNA-resistance SigmaR1 constructs . (Sawyer et al. Dev Cell. 2024) Image exocytosis with VAMP2/3 plasmids to...regions with FUS and HNRNPA1 plasmids . (Shin et al. Cell. 2017) Study amyloid deposition with APOE2 constructs...gene silencing with CRISPRoff-v2.1 . (Nuñez et al. Cell. 2021) Conduct multimodal genetic screens in human...system, peripheral nervous system, vesicular brain cells and more. Measure synaptic transmission with improved...

...others): caveolin 1, also known as CAV 1. Usually, caveolin 1 promotes the cell life cycle progression and ...and spread of cancer cells. Besides these stromal cell modifications, stromal cells are also constantly...how cancer cells influence microenvironments and vice versa, let’s take a look at cancer cell metabolism...inflammation, a noteworthy cell type in the tumor microenvironment is the immune cells. The contradiction is...injecting breast cancer cells into mice, exosomes expressed specific integrins (cell-adhesion proteins) targeting... be transferred to other cells, inducing epigenetic changes in targeted cells. In this case, exosomes ...Friendly” microenvironment cells Not all is bad news – we also have “friendly” cells in microenvironments! ...