...Behan FM, Green T, Krill-Burger J, Beaver CM, Younger ST, Zhivich V, Najgebauer H, Allen F, Gonçalves E, Shepherd...CRISPR Pooled Library Screens Read other CRISPR Blog Posts Resources on Addgene.org Brush up on Your CRISPR...

...Cheng K, Goodwin LO, Morgan JL, Juan WC, Roca X, Ong ST, Bergstrom DE (2018) Viable Mice with Extensive Gene... this process check out our Mouse Modeling blog posts: Part 1: Genetically Engineered Mice and Part 2:...going through conventional gene targeting. It also costs less. With CRISPR, it may cost about $5,000 to generate...

... a fluorescent protein as a scaffold to allow scientists to selectively modulate gene expression....modulate biological activity. Early tools allow scientists to regulate transcription, and additional tools...other activities. By using GFP as a scaffold, scientists can design de novo systems using modular components...

...depositing and recipient scientists to further develop and refine these application lists. The plasmids we use... Protocols Recipient Instructions Antibody Blog Posts Addgene Help Center...

...Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA plasmids designed to target various...gRNA Plasmids Please note that the following table lists both published and unpublished plasmids containing...

...Search to find plasmids available to industry scientists while also adding additional filters such as ...plasmids that are currently available to industry scientists. ID Plasmid Gene/Insert PI Antibodies Addgene...what you would like to see available to industry scientists. Please note this does not guarantee availability...

...Addgene have been produced in-house by Addgene scientists. They are generated using standard methods individually...recommend repeated freeze thaw of antibodies and suggests preparing single-use aliquots before storing at... Protocols Recipient Instructions Antibody Blog Posts Addgene Help Center...

...speed progress towards curing and treating them. Scientists are studying protein aggregation, learning about...or way to prevent progression of PD. Treatment consists mainly of managing symptoms through medication...Factors Find plasmids used to transform iPSCs and fibroblasts into neurons and more. New and Noteworthy: Study...

...Pederson pLH-stsgRNA1.1 64116 Mammalian/Lentiviral U6 none N. meningitidis Pederson pLH-stsgRNA2.1 64117 Mammalian...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR requires that you have to express both a ...Lentiviral U6 none N. meningitidis Pederson pLH-stsgRNA3.1 64118 Mammalian/Lentiviral U6 none N. meningitidis...

...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR system has been adapted to allow tagging...endogenous proteins. This CRISPR/Cas plasmid-system consists of two components: A vector containing Cas9 and...) microhomologies as homology arms. The system consists of two components: An all-in-one CRISPR/Cas9 vector...

...collections related to COVID-19 that may be of use to scientists. Background The Coronavirus Disease-2019 (COVID...from the Coronaviridae family called SARS-CoV-2. Scientists have been working at lightning speed to elucidate...generating SARS-CoV-2 spike pseudotyped virus. It also lists several luciferase and fluorescent reporter plasmids...

... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo...invading phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas)...transcriptional activation of endogenous genes. It consists of three components: A nucleolytically inactive...

...Based on these Cre-lox recombination principles, scientists have developed constructs to activate/inactivate...from Gerald Rubin's lab. Cre-containing Plasmids Scientists have developed ways to tightly control Cre expression...Cre-ERT2 - Tamoxifen inducible. Expressed in fibroblasts mouse Col1A2 Vertebrate Tanaka 111152 Col2a1:...of Gene B. FLEx Switch ∗ : This system allows scientists to utilize recombination elements such as Cre...

...sequences of these affinity reagents available to scientists. Additionally, we have developed a variety of...choose, store, or use your antibody. Antibody Blog Posts Recipient Instructions Addgene Help Center Antibody...

...Protocols gRNA Design Tools CRISPR Blog Posts This page lists plasmids required to practice CRISPR-Cas...

...Protocols gRNA Design Tools CRISPR Blog Posts This page lists plasmids required to practice CRISPR-Cas...

...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays...Consortium: Nuclease Expression Vectors This page lists plasmid vectors designed to express nucleases in...

...Protocols gRNA Design Tools CRISPR Blog Posts This page lists plasmids for practicing the dimeric CRISPR...

... that are available from Addgene's depositing scientists.... that are available from Addgene's depositing scientists. Browse Fluorescent Protein Plasmids by Category...

.... Addgene Blog: Viral Vector Posts : Content from Addgene and scientists across the research community...