...simultaneously with dual labeling when used with two substrates Can be used in live or fixed cells, and for internal...protein tags based on the acyl carrier protein. Substrates are derivatives of Coenzyme A (CoA). These tags... Please see the note below for information on substrates and synthases needed for use of these tags. Plasmids...product portfolio, including the CoA-derivative substrates and the ACP and SFP Synthases. Users may still... their own fluorescent or non-fluorescent CoA substrates by purchasing coenzyme A disodium salt from a...

...nuclease operates as a dimer, cutting in the spacer region between two distinct ZF target sites. (B) A ...species almost as easily as correcting a typo in the latest draft of your dissertation. Well, that will probably...corresponding zinc fingers, potential targetable sites in your average genome are every 500 bp. TAL effectors... a T), but they still have potential targetable sites approximately every 35bp. Researchers are still ...

...pyogenes GFP Vakoc U6>sgRNA(F+E) 59986 Other/Ciona intestinalis BsaI none S. pyogenes Christiaen p(U6a-BsaI-...vectors containing multiple guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette...the expression of multipe gRNAs. Csy4 cleavable cassettes; requires expression of Csy4 (eg Plasmid 53369...gRNA module vectors, multiple gRNA expression cassettes can be assembled using Golden Gate cloning or ...polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG cassettes are assembled into a Cas9 expressing vector. Yang...to rapidly generate multiple sgRNA expression cassettes, which can be assembled into the binary CRISPR...sgRNAs Zebrafish Plasmids for assembly of gRNA cassettes using Golden Gate ligation into Tol2-based pGGDestTol2LC...

...Cell type-biased: Astrocytes - AAV-PHP.AX with GRE or transgenic animal Oligodendrocytes - AAV-PHP.eB with...digital PCR, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector ...LY6A-nonpermissive mouse strains. **AAV1-X1 has not been tested in rats. Abbreviations: AAV, adeno-associated virus...regulatory element; NHP, non-human primate. Image courtesy of the Gradinaru Lab and the Caltech CLOVER Center...

...sequences and gRNA target sites. The first deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR ...plasmids in this CRISPR-Cas tagging system were tested by tagging transcription factors with FLAG in human...plasmids in the following articles provide PCR templates for amplification of the tag (eg GFP, Flag, YFP...elegans . This system uses a 10-day protocol, generates “clean” homozygous mutants with no co-integrated...

...genome engineering. CRISPR Topic Page : Want the latest news on CRISPR? Experts cover CRISPR topics on ... Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused...loci. dCas9-FokI dCas9 fused to FokI nuclease generates a DSB when expressed with two gRNAs. Browse Plasmids...Organism Bacteria C. elegans Drosophila Mammalian Parasites Plant Yeast Xenopus Zebrafish...

... TALE transcriptional activator target sites and then generates individual user-friendly graphical roadmaps...Zebrafish (Sander et al., Nat Biotechnol. 2011) Rats (Tesson et al., Nat Biotechnol. 2011) Human somatic cells...monomeric TALE transcriptional activator target sites and to simplify the construction of TALEN expression...

...Quality Control Each lot of antibody undergoes testing, either in-house or through outside labs, to confirm...storing at -20 °C. Each antibody catalog item is tested for shipping stability by incubating at 37 °C for..., freezing at -80 °C, thawing on ice, and then testing for loss of potency against a non-treated control...recommended assays. If an antibody has passed stability testing, it may ship at room temperature. However, Addgene...

... easy assembly of one or more gRNA expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use...create plant expression vectors containing several cassettes and generate multi-construct transgenic plants...The ABRC collects, preserves, reproduces, and distributes diverse seed and other stocks of Arabidopsis ... (Link opens in a new window) - The initiative promotes interdisciplinary exchange, open technologies,...

...numbers of 8-mer P UF b inding s ites (PBS). These PUF-binding sites recruit PUF (Pumilio/FBF) domains... and resources on using Casilio, as well as the latest sgRNA-PBS sequences and modules JAX press release...appended with multiple copies of Pumilio binding sites (PBS). ID Name Description 71888 pAC1371-pX-sgRNA...

...inducible - VIP vasoactive intestinal peptide MGC13587, PHM27 VIPR1 vasoactive intestinal peptide receptor 1 ...immune response is triggered within the first few minutes of exposure to a pathogen and is the dominant defense...Chemokines recruit innate and adaptive immune cells to sites of inflammation throughout the body. Cytokines Cytokines...its cognate antigen along with co-stimulation activates the T cell, whereas T cell tolerance or anergy... NK cells, macrophages, dendritic cells, and granulocytes. TLR The toll like receptors (TLR) play a key...Chemokines recruit innate and adaptive immune cells to sites of inflammation throughout the body. Symbol Name...chemokine (C-C motif) ligand 5 D17S136E, MGC17164, RANTES, SCYA5, SISd, TCP228 CCL7 chemokine (C-C motif)...

...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...hypermutation in a 100 bp window. This technique creates diverse populations of mutants for directed evolution...adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts adenosine... transcription factor and other protein binding sites. Plasmid Gene/Insert Promoter Selectable Marker ...

...Rabies Virus-Based By Serotype Serotype Testing Panel Serotype Testing Samples small samples of control AAV...CAP-B10, CAP-B22 University of Florida Serotype Testing Panel for the Eye and Brain for enhanced transduction...

... All Antibodies All Antibodies Addgene distributes ready-to-use recombinant antibodies. These monoclonal...recommended applications based on our in-house testing and data provided by trusted outside labs – with... represent the official views of the National Institutes of Health. Need Some Help? Browse some of our...

...induce defined deletions between the two cleavage sites, while the addition of an ssODN donor template can...pU6-BbsI-chiRNA plasmid via the BbsI restriction sites. Design oligos based on the following template: ...known as pHD-DsRed-att). 51026 U6-BbsI-crRNA : Generates crRNA for use in combination with tracrRNA. 51434...

...plasmid, are recombined into a DNA molecule that incorporates sequences from both plasmids. This DNA molecule...for the AdenoBuilder genome assembly system; consolidates the regions present in pAd5-B6 ∆E3-GFP and pAd5... Vogelstein 16407 pAdEasy 2-GFP beta-gal Shuttle Test plasmid that contains β-gal and GFP; contains ∼10Kb...

...Pre-constructed pairs of TALEN plasmids targeting 48 sites in the EGFP reporter gene....large series of engineered TALENs targeted to 48 sites in the EGFP reporter gene ( Reyon & Tsai et al.,...TALENs that were shown to be active at their target sites on an integrated EGFP reporter gene in cultured ...

...transfer vectors you can find reporter plasmids to test whether or not you’ve efficiently generated infectious...recombinases to remove genes flanked by either loxP or frt sites respectively, and empty vectors to express your ...screening and forms blue colonies on LB/Amp/Xgal/IPTG plates – if your insert is successfully cloned into this...

...ID Plasmid Description PI Viral Preps Addgene distributes ready-to-use viral preps made from plasmids in...and targeted to the neuronal soma and proximal dendrites in a viral vector Hillel Adesnik 108422-AAV5 pAAV-CAG-FLEX-Archon1...ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a ...(EF1a) Francois St-Pierre Antibodies Addgene distributes ready-to-use recombinant antibodies made from...

... expressing dCas9-2xAM and gRNA cloned in Bbs I sites. 92221 pLenti_dCas9-2xAM_hIRF-1 Lentiviral plasmid...genome functions.Fujita T, Yuno M, Fujii H. BMC Res Notes. 2018 Jun 14;11(1):387. doi: 10.1186/s13104-018-...epitope tags. Fujita T, Yuno M, Fujii H. BMC Res Notes. 2018 Feb 27;11(1):154. doi: 10.1186/s13104-018-...