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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 137 results for: ica

Showing: 1 - 10 of 137 results

  1. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...fluorescence indicator of K+ for optical imaging (GINKO) Genetically encoded fluorescent indicators for imaging...comparison of a genetically encoded sensor and small molecule indicator: implications for quantification of cytosolic...Javier García-Sancho Calcium Genetically encoded calcium indicators for optical imaging (GECOs), including...and ratiometric indicators An Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011 Sep...Teal genetically encoded calcium sensor T-GECI Blue-shifted genetically encoded Ca(2+) indicator with ...fluorescent cAMP indicator (Flamindo2) Genetically-encoded yellow fluorescent cAMP indicator with an expanded...protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based cAMP indicator applicable to optogenetics...

  2. Antibody Guide

    Type
    Collection
    ...Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods Visualization...signaling molecules and are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments...this can vary significantly based on the antibody and conjugate chosen. Signal Amplification The amount ...single assay. Overview of Antibody Applications Antibody-based applications can be generally classed into ...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...processing steps, such as sonication. Denature proteins, using heat and/or chemicals, and run on an SDS-PAGE...concentrates in the sample. Controls for quantification methods Quantification methods should be run with: a positive...

  3. CRISPR Guide

    Type
    Collection
    ...immunoprecipitation (enChIP) using CRISPR. Biochemical and Biophysical Research Communications . 439 (1), 132–136. PMID:... organisms, but modifications to various Cas enzymes have extended CRISPR applications to increasingly...takes place during the S and G2 phases. Chemically or genetically inhibiting genes involved in NHEJ or upregulating...components, like miCas9 , Cas9-DN1S , or others, can increase HDR efficiency. In most applications, a large portion...genes. Each library typically contains ∼3–6 gRNAs per gene to ensure modification of every target gene...following the amplification protocol specified by the depositing lab. After amplification, you’ll need ...heritability. Not all modifications are stable across cell divisions, and some types of modifications are more frequently...

  4. Brain Initiative Collection

    Type
    Collection
    ...Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production...-DIO-JEDI-2P-WPRE Double floxed genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production...-Kv-WPRE Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production...179464-AAV9 pAAV-hSyn-JEDI-2P-WPRE Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production... the human brain through the development and application of innovative tools enabling large-scale real-time...or when BRAIN Initiative grants are noted in publications associated with Addgene materials. Plasmids ... 113049-AAV9 pAAV-hSyn-GRAB_DA1m express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_...

  5. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a transcriptional...Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional...Selectable Marker PI Publication Epigenetics To make targeted epigenetic modifications, researchers have ... Gene/Insert Selectable Marker PI Publication Purify A catalytically inactive Cas9 (dCas9) can be used...Insert Promoter Selectable Marker PI Publication Visualize A catalytically inactive Cas9 (dCas9) fused to a...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function Genome Editing...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...

  6. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...using AAV for research is that it is replication-limited and typically not known to cause disease in humans...controlled by tTA-TRE amplification system Viviana Gradinaru 103006 pUCmini-iCAP-PHP.S PHP.S AAV packaging...controlled by tTA-TRE amplification system Viviana Gradinaru 127847 pUCmini-iCAP-PHP.V1 PHP.V1 AAV packaging...controlled by tTA-TRE amplification system Viviana Gradinaru 175004 pUCmini-iCAP-AAV.CAP-B10 B10 AAV packaging...controlled by tTA-TRE amplification system Viviana Gradinaru 175005 pUCmini-iCAP-AAV.CAP-B22 B22 AAV packaging...controlled by tTA-TRE amplification system Viviana Gradinaru 185136 pUCmini-iCAP-AAV.MaCPNS1 MaCPNS1 AAV...controlled by tTA-TRE amplification system Viviana Gradinaru 185137 pUCmini-iCAP-AAV.MaCPNS2 MaCPNS2 AAV...

  7. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...therapeutic applications. Despite the ethical controversies surrounding non-research applications, it’s clear...just as useful (if not more so) for research applications, eclipsing past genome engineering technologies...to destroy the phages upon re-exposure. More specifically, specialized Cas proteins snip foreign DNA into...number/types of Cas proteins. Makarova et al. ’s classification defines 5 types and 16 subtypes based on shared...for CRISPR genomic editing. Genome Engineering Applications In 2012, Jinek et al. first demonstrated that... PubMed lists more than 6,300 CRISPR-related publications, many of which detail work to improve the tool...various species, as well as the development of new applications. The first CRISPR papers described two main ...

  8. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...plasmids containing only the elements needed for replication in the host cell and are designed for a user ...Protein Function: Viral Vector Delivery, Genome Modification, mRNA Regulation, and More Selectable Markers...collection page Zebrafish CMV, h2afv, XlEef1a1 See our dedicated Zebrafish Plasmids and Resources collection page...Additionally, tags are commonly added to aid in protein purification. Just remember to remove the stop codon for ...myristoylation signal GST Protein purification pEBG - N-terminal GST for mammalian...expression GST plasmids made by Addgene MBP Protein purification pET28-MBP-TEV - Bacterial expression with MBP-TEV...MBP-tagged fusion proteins in E. coli SUMO Protein purification pCIOX - Bacterial expression vector for His8...

  9. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Catalytically inactive CRISPR plasmids for purification of target loci....conventional methods for genomic purification, CRISPR-based purification methods are more straightforward and...Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...chromatin immunoprecipitation (ChIP) to allow purification of any genomic sequence specified by a particular...DNA-binding molecule-mediated ChIP) system, catalytically inactive dCas9 is used to purify genomic DNA... can be fused to dCas9 or gRNA for efficient purification. Various epitope tags including 3xFLAG-tag, ...

  10. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a transcriptional... Plasmid Gene/Insert Promoter PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional...population. ID Plasmid Promoter PI Publication Purify A catalytically inactive Cas9 (dCas9) can be used...than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome Editing...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...gRNA Design Tools CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein...opposite strand) mutations. ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...
Showing: 1 - 10 of 137 results