...PHP.V1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175004 pUCmini-iCAP-AAV.CAP-B10 ... B10 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175005 pUCmini-iCAP-AAV.CAP-B22 ...and PHP.eB Cap controlled by tTA-TRE amplification system Viviana Gradinaru 103006 pUCmini-iCAP-PHP.S PHP.S...and PHP.S Cap controlled by tTA-TRE amplification system Viviana Gradinaru 127847 pUCmini-iCAP-PHP.V1 PHP.V1... and B22 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 185136 pUCmini-iCAP-AAV.MaCPNS1... MaCPNS1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 185137 pUCmini-iCAP-AAV.MaCPNS2...plasmid (non-standard), expressing Rep2 and MaCPNS2 Cap controlled by tTA-TRE amplification system Viviana...

....V1 MaCPNS1 MaCPNS2 CAP-B10 CAP-B22 AAV9-X1.1 As part of our Viral Vector Packaging Service , Addgene ...Pubmed 35643078 (Link opens in a new window) CAP-B10, CAP-B22: Goertsen, Flytzanis, et al., 2022 Pubmed...Control Boyden CAP-B10 These viral vector preparations were produced with the pUCmini-iCAP-AAV.CAP-B10 plasmid...Control Boyden CAP-B22 These viral vector preparations were produced with the pUCmini-iCAP-AAV.CAP-B22 plasmid...plasmid (Addgene #175004) . Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP...plasmid (Addgene #175005) . Browse Available CAP-B22 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP...AAV Caltech Systemic Capsids Viral Vector Packaging Service: Caltech Systemic Capsids PHP.eB PHP.S PHP.V1...

... and human Jonathan Ting Viviana Gradinaru 37825-CAP-B10 pAAV-CAG-GFP AAV expression of GFP from the CAG...CAG promoter Edward Boyden Viviana Gradinaru 37825-CAP-B22 pAAV-CAG-GFP AAV expression of GFP from the CAG...CAG promoter Edward Boyden Viviana Gradinaru 37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the...CAG promoter Edward Boyden Viviana Gradinaru 37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the... Preps ID Viral Prep Description PI (Cargo) PI (Capsid) 214869-PHPeB AiP15140: pAAV-AiE0873m_3xC2-minBG-CoChR-EGFP-WPRE3...

...AAV9 AAVrh10 Retrograde AAV PHP.eB PHP.S PHP.V1 CAP-B10 CAP-B22 MaCPNS1 MaCPNS2 AAV9-X1.1 Promoter CAG CaMKIIa... GFP Constitutive 1, 2, 5, 8, 9, rg*, PHPeB, CAP-B10, CAP-B22, MaCPNS1, MaCPNS2, AAV9-X1.1 Boyden 44332...

...neurons Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1, MaCPNS1, MaCPNS2, CAP-B10, CAP-B22 University of Florida...

...plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral helper sequences...intensity of the viral capsid proteins are analyzed. The abundance of viral capsid proteins as a fraction...the relative stoichiometric ratios of the viral capsid proteins VP1, VP2 and VP3. Samples of viral preparations...non-purified AAV subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are shown relative to...

...TALENs Full Target Site (5' to 3'; half-sites in CAPS, spacer in lowercase) aak1 TAL3208 & TAL3209 TCTTTGACTCCCGGAGAGagctggtgggctccgggCCAGGTTCTGGAGGGGGA...

...pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6 and IRES-dependent ECFP-HA-His6...Gateway vectors) pCAP03-acc(3)IV - Facilitate the capture and expression of bacterial gene...

...antibodies are capable of recognizing and binding to epitopes on antigens, they are also capable of recognizing...In a sandwich ELISA, a capture antibody, bound to a surface, is used to capture the protein of interest...also be included. Antibody Applications - Capture Methods Capture methods such as immunoprecipitation (IP...variation of IP uses antibodies to capture protein:DNA complexes. After capture, the DNA is released from the...fragmentation of the DNA. Controls for capture methods Capture methods should use a positive control and...methods. Capture MS validation This method uses peptide identification by proteomics (Capture MS) to validate...Amplification Application Overview Quantification Methods Capture Methods Visualization Methods Cell Sorting Methods...

...iCre CAG Mammalian Capecchi 113849 pCAGGS-pac-T2A-iCre iCre CAG Mammalian Capecchi 116879 CAG-Cremyc-2A-GFP...Mammalian Capecchi 125749 pCAG-sfGFP-iCre-ERT2 iCre-ERT2 - Tamoxifen inducible CAG Mammalian Capecchi 125821...Mammalian Capecchi 125822 pCAGGS-mTagBFP2-T2A-iCre (v2) TagBFP, iCre CAG Mammalian Capecchi 126006 pAAV2...domain CMV Mammalian Green 12496 p270 pCMV-CRE-M-AR(LNCaP) CREM fused to mutant AR ligand binding domain CMV...unskippable linker) - Tamoxifen inducible CAG Mammalian Capecchi 128167 pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2...GSAx9 linker) - Tamoxifen inducible CAG Mammalian Capecchi 128168 pCAGGS-mTagBFP2-T2A-iCre-ERT2 iCre-ERT2...-terminus) - Tamoxifen inducible CAG Mammalian Capecchi 128171 pCAGGS-mTagBFP2-T2A-iCreERT2 (PV) iCre-...

..., and dogs. This serotype was derived from AAV2 capsid and developed by Arun Srivastava’s lab. AAV2(trpYF...Arun Srivastava’s lab. It is derived from the AAV2 capsid and contains the following mutations: Y444F, Y500F...Arun Srivastava’s lab. It is derived from the AAV6 capsid and contains the following mutations: Y705F, Y731F...19066593 Other citations include: Bogner, et al. 2015. Capsid Mutated Adeno-Associated Virus Delivered to the...Kanaan, et al. 2017. Rationally Engineered AAV Capsids Improve Transduction and Volumetric Spread in the...al. 2016. Microglia-specific targeting by novel capsid-modified AAV6 vectors. Mol Ther Methods Clin Dev...

...targeting the SARS-CoV-2 nucleocapsid protein (Brian Geiss). Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] - ...monoclonal antibodies that target SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that...COVID-19 research. Antibodies Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] - Clone mBG17, recombinant mouse...monoclonal antibody targeting the SARS-CoV-2 nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids...RBD mutants that can be used to identify antibody escape mutants and for comprehensive mapping. Some of ...

... others work to increase Cas9’s proofreading capabilities. No matter the method, increased fidelity enzymes...by the Feng Zhang lab ) combine the large-scale capacity of transposases with the precise DNA-targeting...programmability for different genomic loci, and the capability to detect multiple targets simultaneously. CRISPR...and biotin tags, or an anti-Cas9 antibody. The CAPTURE system, developed by the Jian Xu lab , utilizes... Cas12a also has more condensed multiplexing capabilities , as it can process multiple gRNAs under a single...carrying an ADAR truncation that retain RNA editing capabilities but are small enough to be packaged in AAV particles...two nuclease domains inactivated. This enzyme is capable of cleaving only one strand of target dsDNA. Off-target...

...they are capable of differentiating into every other cell type in the body and have the capacity to give...are proliferative, unspecialized cells with the capacity to differentiate into many different cell types... Acad Sci U S A. 2014 Jul 22;111(29):10678-83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged...

...sometimes require preps beyond our current in-stock capabilities. To meet that need, our Packaged on Request ...globally connected scientific community. The Data Hub captures valuable knowledge that often goes unshared, bridging...troubleshooting and results that complement published data, capturing the insights that often go unshared. Help Center...

...Mammalian (pEGFP-C2) GAP Acap1 9744 740 Mammalian (pFLAG-CMV2) GAP Acap2 23527 778 GAP Acap3 116983 834 GAP Agap1...

...nickase fused to a reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and... to immunoprecipitate FLAG-tagged Cas9. In the CAPTURE system, Cas9 is tagged with a biotin acceptor site...streptavidin affinity purification. For both enCHIP and CAPTURE, design your gRNA sequence to direct dCas9 to a...

...polypeptide 1 (pituitary) receptor type I PAC1, PACAPR, PACAPRI ADIPOQ adiponectin, C1Q and collagen domain...receptor 1 FLJ41949, HVR1, II, PACAP-R-2, RDC1, VAPC1, VIPR, VIRG, VPAC1, VPCAP1R VIPR2 vasoactive intestinal...C5R1, CD88 CAMP cathelicidin antimicrobial peptide CAP18, CRAMP, FALL-39, FALL39, HSD26, LL37 CCBP2 chemokine...MGC138386, NDI, V2R AZU1 azurocidin 1 AZAMP, AZU, CAP37, HBP, HUMAZUR, NAZC BDNF brain-derived neurotrophic...CGRPR, CRLR CAMP cathelicidin antimicrobial peptide CAP18, CRAMP, FALL-39, FALL39, HSD26, LL37 CAT catalase...intestinal peptide receptor 2 FLJ16511, VPAC2, VPCAP2R XCL1 chemokine (C motif) ligand 1 ATAC, LPTN, LTN, SCM...

...adenoviruses in research include their large packaging capacity (>8 kb), high titers, and high levels of transgene...substantial immune response induced by the viral capsid. Adenoviruses can be produced by several methods...

...genome. AAVs are limited by their small packaging capacity. Adenovirus Plasmids | Guide Adenoviruses are ...linked above. Virus Expression Genome Packaging Capacity Virus Size (nm) Cells Infected Target Cell Genome...