We narrowed to 39 results for: CIT

...Expression mCitrine 516 529 59 5.7 Monomer (A206K) mCitrine-N1 - Mammalian Expression mCitrine-C1 - Mammalian...Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-pBAD - Bacterial...Mammalian Expression pCS2+8CmCitrine - Zebrafish/Xenopus/Worm/Sea urchin pCS2+8NmCitrine - Zebrafish/Xenopus...Photoactivatable (Off to On) Excitation and Emission wavelengths after activation. Protein Excitation (nm) Emission ...vector type, popularity, and more. Blue/UV Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...Bacterial Expression Return to top Cyan Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...Bacterial Expression Return to top Green Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...

...pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid receptor (KOR) - Inhibition IRES mCitrine Cre-dependent 8 Bryan...-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine Cre-dependent 8 Bryan...-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine Cre-dependent 8 Bryan...pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine none 2, 8 Bryan Roth ...pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine rM3D(Gs) - Activation HA fusion and IRES mCitrine none 5 Bryan Roth 50478...228902 pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine HCAD(Gi) - Inhibition mCitrine Cre-dependent 8 Bryan Roth No available...element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity Cre-dependent Flp-dependent...

...References for each serotype can be found below under Citations. AAV2(Y444F) The AAV2(Y444F) serotype is demonstrated...Beta actin (CBA) GFP Control Sergei Zolotukhin Citation Information AAV2(Y444F) When using the AAV2(Y444F...publications, please acknowledge Arun Srivastava and cite Petrs-Silva, et al. 2009. High-efficiency transduction.... 2009 Mar;17(3):463-71. PMID: 19066593 Other citations include: Bogner, et al. 2015. Capsid Mutated Adeno-Associated...publications, please acknowledge Arun Srivastava and cite Petrs-Silva, et al. 2011. Novel properties of tyrosine-mutant...Ther . Feb;19(2):293-301. PMID: 21045809 Other citations include: Chen, et al. 2014. Reprogramming adipose... Dev . Sep;26(3):165-76. PMID: 26390090 Other citations include: Scalabrino, et al. 2015. Intravitreal...

... every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most...targeting multiple genomic locations or enhancing specificity, efficiency, or flexibility of the Cas enzyme...Vectors Engineering the Cas9 Endonuclease CRISPR specificity is determined by both the gRNA sequence and the... design, engineered Cas9s can enhance CRISPR specificity. SpCas9 (from Streptococcus pyogenes ) is the...many modified versions engineered to increase specificity and nuclease activity available. Engineered Cas9s...cleavage of target DNA. Cas9n increases target specificity. Since two nickases targeting opposite DNA strands...fidelity of Cas9 Researchers have also enhanced specificity by engineering Cas9 into high fidelity Cas9s ...

... of 10 Antigen Specificity Antibodies are available in two types of antigen specificity: monoclonal, meaning...necessary in cases where extreme specificity is important. Their epitope specificity allows binding to a specific... Guide Production Structure Isotypes Antigen Specificity Other Affinity Reagents Handling Visualization...variety in the antibody allowing for their extreme specificity occurs in the B cell itself prior to antibody..., and can be used to increase flexibility or specificity in antibody-based methods. Antibodies can recognize...isotype-specific or even isotype subclass-specific. This specificity can be used to ensure secondary antibodies bind...cost-effective. They are useful when epitope specificity is not a concern or in cases where you may want...

...Amara , Susan Ingram Citrate Nanosensors for in vivo measurements of citrate (CitA) Engineering genetically...inverse fluorescent biosensor for citrate (Citron/Citroff), mitochondrial or cytosolic High-Performance Intensiometric...measurements of citrate concentrations. PLoS One. 2011;6(12):e28245. Uwe Sauer Citrate Direct or inverse...Magnesium MagFRET-1 Mg 2+ sensor, based on Cerulean/Citrine MagFRET: The First Genetically Encoded Fluorescent...Inverse-Response Genetically Encoded Biosensors for Citrate. ACS Cent Sci. 2020 Aug 26;6(8):1441-1450. Robert... reveals a role for pH regulation in hair cell toxicity and function. Dis Model Mech. 2014 Jul;7(7):847...Thomas Knopfel Voltage Detection of single action/excitatory potentials in individual neurons and dendrites...

...N1 , mVenus N1 mTFP1 mCitrine 462 0.85 529 94,000 0.74 5.9 2.6 mTFP1-N1 , mCitrine-pBAD EGFP mCherry 488... energy is non-radiatively transferred from an excited donor fluorophore to an acceptor. Because this ...using fluorescence microscopy or spectroscopy by exciting the donor and detecting the emission of the acceptor..., the fluorescence lifetime (the time between excitation of the fluorophore and emission of a photon) ...-ShadowY , EGFP-ShadowY λ Dex : Donor maximum excitation wavelength (nm), QY D : Donor quantum yield, ...Overlap integral of the donor emission and acceptor excitation spectra (×10 15 M -1 cm -1 nm 4 ). Values obtained...

...Roger Tsien 54553 54723 mTFP1-pBAD mCitrine-pBAD mTFP1 (donor) mCitrine (acceptor) FRET/Dual FRET Robert...ECFP (donor) Citrine (acceptor) FRET Wolf Frommer 65617 pFLIP42 mCerulean (donor) Citrine (acceptor) FRET...the principle that when a donor fluorophore is excited, it can transfer its energy to an acceptor fluorophore...fusing each of them to two FPs whose emission and excitation spectra overlap. That way if your proteins of...the donor and the acceptor fluorophore by only exciting the donor. In BiFC, proteins of interest are fused... can be used for determining the DNA-binding specificity of proteins such as transcription factors. Addgene...

...transgene, packaging capacity, and size. Vector Type Expression Genome Packaging Capacity Virus Size Cells...gene delivery in vivo because of their mild immunogencity. These viral vectors can direct long-term transgene...genome. AAVs are limited by their small packaging capacity. Gamma-retroviral Vector Guide | Gamma-retroviral...Plasmids Adenoviral vectors have high packaging capacity, high transduction efficiency, and high but transient...preferred for in vivo studies because of their low immunogenicity. Viral vectors also vary in the composition...

...switch system takes advantage of the orientation specificity of Cre and the different types of target sites...target site cassette in a double-crossover event. Toxicity Cre-lox recombination is a very useful tool, but...such as Cre-only lines to account for cellular toxicity in your system. Using inducible Cre systems in...-target events and the systemic effects of Cre toxicity. Leakiness Leaky expression, where the gene of...number of recombination events but can improve specificity in targeted neuronal populations. Read our blog...

...many of which detail work to improve the tool’s specificity, orthogonality, and multiplexibility in various...endogenous loci with unprecedented speed and specificity. Figure 2: An overview of CRISPR and NHEJ/HDR...Joung JK. 2014. Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol . ...Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature . 523(7561):481-5. PMID: 26098369 Komor...Rationally engineered Cas9 nucleases with improved specificity. Science . 351(6268):84-8. PMID: 26628643 Wang...

... to recognize their targets with fairly high specificity. Fifteen years of research has shown that when...zinc fingers within an array can reduce their specificity. Two years ago, groups led by Jens Boch at the...though NN can also bind adenenine with lower specificity). With the template for studying this type of...likelihood of context having a negative effect on specificity is on par with ZF arrays, if not slightly lower...

...developed, including additional fluorophores, excitatory and inhibitory opsins , genetically-encoded calcium...Flp AND NOT Cre Yes Dual recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations...neuron glutamate cotransmission evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons...Andrási T, Deisseroth K, Holmes A, Hájos N. 2019. Excitation of diverse classes of cholecystokinin interneurons...

...MGC126648 CALCB calcitonin-related polypeptide beta CALC2, CGRP-II, CGRP2, FLJ30166 CALCR calcitonin receptor...MLT1 NFAT5 nuclear factor of activated T-cells 5, tonicity-responsive KIAA0827, NF-AT5, NFATL1, NFATZ, OREBP...component 5a receptor 1 C5A, C5AR, C5R1, CD88 CALCA calcitonin-related polypeptide alpha CALC1, CGRP, CGRP-I...receptor CRT, CTR, CTR1 CALCRL calcitonin receptor-like CGRPR, CRLR CAMP cathelicidin antimicrobial peptide ...somatostatin receptor 5 - ST2 suppression of tumorigenicity 2 - STC1 stanniocalcin 1 STC STC2 stanniocalcin...interleukin 9 receptor CD129 ST2 suppression of tumorigenicity 2 - TXLNA taxilin alpha DKFZp451J0118, IL14...polypeptide-related sequence B PERB11.2 NCR1 natural cytotoxicity triggering receptor 1 CD335, FLJ99094, LY94,...

...are proliferative, unspecialized cells with the capacity to differentiate into many different cell types...every other cell type in the body and have the capacity to give rise to an entire organism. iPSC technology...which lower the risk of mutation and minimize tumorigenicity, an inherent disadvantage of iPS cell technology...Neurons Lentiviral Human Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor ...

...imaging tools in this blog post . Far-Red Protein Excitation (nm) Emission (nm) Brightness Plasmids mCardinal...Crimson pAAV-CAG-E2-Crimson Near-Infrared Protein Excitation (nm) Emission (nm) Brightness Plasmids emiRFP670... 720 5.8 piRFP720-N1 Photoactivatable Protein Excitation (nm) Emission (nm) Activation (nm) Brightness...

...High-fidelity Cas enzymes increase specificity Dual-nickase approach increases specificity but is less efficient Each...be able to completely avoid them. To increase specificity, consider using an engineered Cas enzyme with...use in the lab, but AAVs exhibits the lowest immunogenicity and are one of the least toxic methods for ...

... recombinant monoclonal antibodies with high specificity for critical axon guidance signaling proteins...neurotransmission, synapse formation, neural connectivity, plasticity and neurological disorders. The initial collection...Integrin antibodies exhibit distinct binding specificities — recognizing individual α or β subunits, or...

...Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden Citation Information When using the Caltech serotypes in...publications, please acknowledge Viviana Gradinaru and cite as follows: PHP.eB, PHP.S: Chan et al., 2017 Pubmed...L4 - AAV.CAP-B10 with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For...

...non-coding RNA elements capable of programming the specificity of the CRISPR-mediated nucleic acid cleavage....10.1126/science.1231143. PubMed . DNA targeting specificity of RNA-guided Cas9 nucleases. Hsu PD, Scott DA...RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Ran FA, Hsu PD, Lin CY, Gootenberg JS, Konermann...