We narrowed to 73 results for: BASE;

... many FRET-based biosensors in Addgene's Biosensors Collection . Generate custom FRET-based biosensors...plan your next FRET experiment. Looking for FRET-based biosensors? Check out our Biosensors Collection ...stoichiometry of protein interactions using image-based quantitative two-hybrid FRET Positive and negative...10 15 M -1 cm -1 nm 4 ). Values obtained from FPbase.org FRET calculator (Link opens in a new window) ...Link opens in a new window) External Resources FPbase.org FRET calculator (Link opens in a new window) ...Link opens in a new window) Fluorescent Biosensor Database (Link opens in a new window) designed by Eric ...

...before the sgRNA scaffold. The oligos are designed based on the target site sequence (20bp) and need to be... into the CRISPR array. The oligos are designed based on the target site sequence (30bp) and need to be...before the sgRNA scaffold. The oligos are designed based on the target site sequence (21-22bp) and need to... of annealed and phosphorylated oligos designed based on the target site sequence (20bp). CRISPR-Cas9 ... of annealed and phosphorylated oligos designed based on the target site sequence (20bp). #60230 - AAV... of annealed and phosphorylated oligos designed based on the target site sequence (20bp). Return to top...activate all known coding isoforms form the RefSeq database (23,430 isoforms) for gain-of-function screening...

...increase flexibility or specificity in antibody-based methods. Antibodies can recognize and bind other...produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies are necessary .... The complexity of this can vary significantly based on the antibody and conjugate chosen. Signal Amplification...assay. Overview of Antibody Applications Antibody-based applications can be generally classed into four ...Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay that uses standard curves to quantify exact...strategy. Figure 5: Immunoprecipitation using bead-based antibody capture to isolate specific proteins from... cells are then slotted in a specific direction based on the emission(s) read by the machine. Controls...

...codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al., 2004; Lee...overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007) containing...

...Tet-Advanced transactivator (developed by Clontech, based on Resnitzky et al., 1994). TRE3G promoter : also...Tet-On inducible RNAi with miR-E (miR-30 variant)-based backbone rtTA-Advanced TRE3G Johannes Zuber 60495...vector for tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9...dCas13d-dsRBD-APEX2 RNA-protein interaction mapping via Cas13-based APEX targeting Alice Ting 85040 pK170.AAV-TRE-Cre-WPRE...Schiedlmeier, B. (2011). Retroviral and transposon-based tet-regulated all-in-one vectors with reduced background...

...Tracing These AAV encode tools for rabies virus-based monosynaptic tracing. These AAV can be used with...AAV1 Ian Wickersham Introduction to Rabies Virus-based Monosynaptic Tracing The core idea of the system...

...CRISPR resources can be found below: Base Editing RNA Editing CRISPR-based DNA methylation editing system -...occurs. Diagnosis of Rett syndrome is currently based on specific clinical diagnostic criteria, defined...the search bar or sort buttons to find plasmids based on: Expression system (mammalian or bacterial) Insert...Resources Research Databases (Link opens in a new window) InterRett - international online database of clinical...syndrome (Link opens in a new window) RettBase - MECP2 mutation database (Link opens in a new window) OMIM...: 16832102 Krishnaraj et al. 2017. RettBASE: Rett syndrome database update. Hum Mutat . 38, 922–931. (...

...vectors and a reporter vector for mammalian cell-based validation assay that are designed for use with ...through in vitro transcription as an alternative. Based on transient double-selection of transfected cells...TALEN kit. This backbone is suitable for the TALEN-based generation of germline mutations in Bombyx mori ...

...genome editing. (Marquart et al. Nat Methods. 2024) Base edit human mitochondrial DNA using mitoBEs . (Yi...screens in human iPSC-derived neurons with a CRISPRi-based platform . (Tian et al. Neuron. 2019) See More AAV... information on Addgene's collection of plasmid-based antibodies, including monoclonal antibodies, scFvs...

...selection. Ie-Ming Shih 25737 pSLIK-Hygro 3rd Tet-based inducible shRNA or cDNA expression, gateway plasmid...mir-RUP-PheS 3rd Lentiviral shRNA plasmid (pGIPz based) for inducible knockdown, multicistronic design ...1733 pCI-VSVG VSV-G recommended for the FELIX (FIV-based) system. Garry Nolan 8454 pCMV-VSV-G VSV-G-expressing...

...currently available. You can search or sort this table based on plasmid name, gene/insert, and more. ID Plasmid...

... Figure 2 ). The Bogdanove group also hosts web-based software to help scientists find potential TAL effector...assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL...

...Addgene's repository. You can search or sort this table based on plasmid name, gene name, in-stock viral prep ...AAV production. You can search or sort this table based on plasmid name, capsid, and more. ID Plasmid Gene...

...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ...

...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets....

... find SARS-CoV-2 and common coronvirus plasmids based on: Gene insert (e.g. spike, envelope, nsp3, OC43...

... Boye and cite Boye, et al. 2020. Novel AAV44.9-Based Vectors Display Exceptional Characteristics for ...

...Flag for bacterial expression pFA6a vectors - PCR-based C-terminal tagging in S. cerevisiae pCS2FLAG - N...expression pnCasPA-BEC - A cytidine deaminase-mediated base-editing plasmid in Pseudomonas species Vectors for...agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector GFP Varies pMKO.1 GFP...

...conventional methods for genomic purification, CRISPR-based purification methods are more straightforward and...

...CRISPR Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing Samples Small samples ...