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We narrowed to 73 results for: CaS;

Showing: 21 - 40 of 73 results

  1. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems...the fusions, modifying the linker region between Cas variant and deaminase to adjust the editing window...are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase...editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor. Both ... lab and CRISPR-X from the Bassik lab , in which Cas9 is fused to activation-induced cytidine deaminase...

  2. Plant Plasmids and Resources

    Type
    Collection
    ...Yinong Yang. The MoClo CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA...MoClo-compatible zCas9i Cloning Kit includes a high efficiency intron-optimized SpCas9-coding gene, zCas9i, and an...assembly of one or more gRNA expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice. ... an intronized Cas9 series of vectors and DNA modules for assembly of CRISPR/Cas9 constructs - Sylvestre...CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants - Niko Geldner. CRISPR-Cas9 vector...development. The TapRoot - Podcast (Link opens in a new window) - The Taproot is a podcast to understand how scientific...plasmids expressing different functional variants of Cas9 and empty gRNA backbones. A few examples of useful...

  3. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...modifications, researchers have fused catalytically dead dCas9 to epigenetic modifiers. Design your gRNA to target...

  4. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...that of the Cas protein present on the plasmid. gRNA plasmids that do not co-express a Cas protein require...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems... CRISPR requires that you have to express both a Cas protein and a target-specific gRNA in the same cell.... Single plasmids containing both the gRNA and a Cas protein act as an all-in-one vector, but their function... plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that can cut, activate... guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette using the Golden Gate ...accessory pack is available for dCas9 and FokI-dCas9. Yamamoto Multiplex CRISPR/Cas9-based genome engineering...

  5. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...target the dCas9-repressor to your specific locus. Want more information on the wide variety of Cas enzymes...Tools CRISPR Blog Posts Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity...

  6. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of CRISPR systems with...VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing the...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...E488Q) adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts...guanosine, the result is an A->G change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence...

  7. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...target the dCas9-activator to your specific locus. Want more information on the wide variety of Cas enzymes...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...Design Tools CRISPR Blog Posts Catalytically dead dCas9 fused to a transcriptional activator peptide can...specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity...

  8. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of CRISPR systems with...sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. In bacteria...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require that each DNA target have...to attentuate RNA levels. In mammalian systems, Cas13a does not exhibit the collateral RNA degradation...

  9. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...CRISPR/Cas plasmids for labeling and visualization of genomic loci.... inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have turned dCas9 into a customizable...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  10. CRISPR Plasmids - Parasites

    Type
    Collection
    ...help of Scott Lindner . Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and forward...forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some...

  11. Zhang Lab CRISPR Page

    Type
    Collection
    ...Lab General Cloning Protocol 237.2 KB SpCas9 Plasmids The CRISPR/Cas system can be implemented in mammalian...Return to top References SpCas9 plasmids Multiplex Genome Engineering using CRISPR/Cas Systems. Cong L, Ran...hosts contain a combination of CRISPR-associated (Cas) genes as well as non-coding RNA elements capable...efficiency. SpCas9 and SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n, D10A nickase...Protocols 2013). SpCas9 Cas9: The PX551 plasmid (AAV-SpCas9, #60957) contains a single cassette expressing ...plasmid expressing SpCas9 alone without sgRNA. Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase...plasmids contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and the single guide RNA...

  12. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems...species or PAM binding variant of Cas9. For instance, wild-type SpCas9 must be used with targets that are...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...increases off-target effect. Which species or variant of Cas9 ( S. pyogenes , S. aureus etc.) was this gRNA sequence...

  13. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Sensor for Ex Vivo and In Vivo Imaging of Ca Dynamics in High-Ca Organelles. Cell Chem Biol. 2016 Jun 1....Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...calcium sensor T-GECI Blue-shifted genetically encoded Ca(2+) indicator with enhanced two-photon absorption...green, red or blue/green) Imaging intraorganellar Ca(2+) at subcellular resolution using CEPIA. Nat Commun...fluorescent CEPIA indicators for visualization of Ca(2+) dynamics in mitochondria. Sci Rep. 2020 Feb 18...CEPIA1er ER-mitochondria tethering by PDZD8 regulates Ca(2+) dynamics in mammalian neurons. Science. 2017 ...Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins...

  14. Worm Expression Resources

    Type
    Collection
    ...found at the Goldstein Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen Lab. A collection of plasmids... via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified Cas9, such as that...feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific transgenic...worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous...that incorporate a self-excising drug selection cassette (SEC). More information regarding this kit can...targeting vectors for tag insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR ...that expressed from pHO4d-Cas9 from Michael Nonet. Protocol can be found at the (Link opens in a new window...

  15. Zebrafish Plasmid Collection

    Type
    Collection
    ...Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression...Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector System...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference...morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful...Knock-in tagging - Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes...use of zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been experimentally validated...

  16. Luciferase Plasmid Collection

    Type
    Collection
    ...fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Luminopsins : Luciferase-opsin...signaling. TRUPATH Kit , ONE-GO Biosensors Kit , and G-CASE : BRET-based sensors for reporting on GPCR activity...

  17. Deisseroth INTRSECT Collection

    Type
    Collection
    ... PubMed (Link opens in a new window) Marcinkiewcz CA, Mazzone CM, D'Agostino G, Halladay LR, Hardaway ...

  19. Lentiviral Prep Service

    Type
    Collection
    ...Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins... 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...backbone. Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused...Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A ...lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 ...-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs targeting 19,114 human...plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used to...

  20. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Anti-CASPR/Neurexin IV [K65A/2R] CASPR/Neurexin IV Mouse IgG2a 206606 Anti-CASPR2 [K67/11R] CASPR2 Human...190491 CASK scFv [K56A/50] K56A/50 scFv CASK Human Mouse 190492 CASK scFv [K56A/57] K56A/57 scFv CASK Human...177445 Anti-CASPR/Neurexin IV [K65/35R] CASPR/Neurexin IV Rat Mouse IgG2a 177446 Anti-CASPR2 [K67/25R] ...219448 Anti-CASPR/Neurexin IV [K65A/30R] CASPR/Neurexin IV Rat Mouse IgG2a 219449 Anti-CASPR/Neurexin IV...SAP97 Rat Mouse IgG2a 128625 Anti-CASPR/Neurexin IV [K66/38R] CASPR/Neurexin IV Rat Mouse IgG2a 128626...Pan-KChIP K+ channel Mouse IgG2a 177443 Anti-CASK [K56A/50R] CASK Human Mouse IgG2a 177444 Anti-KChIP2b K+...Synaptotagmin-6 Mouse Mouse IgG2a 188217 Anti-CASK [K56A/57R] CASK Human Mouse IgG2a 188218 Anti-Kv2.1 K+ channel...
Showing: 21 - 40 of 73 results