...mammalian tissue culture cells, the R-mAbs are secreted into the culture media and can be collected for...domains to obtain recombinant hybridomas, which secrete antibodies as Fab' fragments, isotype-switched ...antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining...libraries. Immunol Lett. 2015 Oct;167(2):95-102. Joanna Bereta Sybody Generation Toolbox for making synthetic ...-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions for other plasmids...

... zebrafish cell surface receptors and secreted proteins. Return to top Other Resources Addgene Blog Posts...described below . ID Plasmid Description Gene/Insert PI Return to top Highlights of Zebrafish Tools in our Collection...-binary expression system for use in zebrafish - Bret Pearson Lab MAZe: a tool for mosaic analysis of ...for analyzing single cell genomics in zebrafish. Return to top Do you have suggestions for other plasmids...

...Browse all Control AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova...serotypes. Serotype AAV1 AAV2 AAV5 AAV8 AAV9 AAVrh10 Retrograde AAV PHP.eB PHP.S PHP.V1 CAP-B10 CAP-B22 MaCPNS1...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? ...viruses would be helpful to the scientific community. Return to top...

...continually growing. ID Plasmid Description Industry PI Return to top Other Coronavirus Plasmids: SARS-CoV, MERS...materials are available only in the US at this time. Return to top Plasmids Encoding Mammalian Genes or Inserts...related plasmids see our Immunology Resource Guide . Return to top General Plasmid Tools SHERLOCK and DETECTR...Transcriptase plasmids from Andrew Ellington's lab. High-surety isothermal amplification and detection of SARS-...lab to ask them to deposit the plasmid with us. Return to top Pooled Libraries SARS-CoV-2 Spike (S) Ectodomain...

...interest by fusing it to a fluorescent protein. FRET Find FRET pairs and standards to study protein-protein... empty backbones. Fluorescent Protein Kits cpFRET Kit: FRET based biosensors - Pertz Fluorescent Protein...

...HIV-based lentiviral particles Murine leukemia-based retroviral particles Vesicular stomatitis virus particles...Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus Envelope and Packaging Plasmids Reporter Plasmids...vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly luciferase...Resources Ginkgo Bioworks COVID-19 Plasmid Collection Return to top Do you have suggestions for other plasmids...

...viral service encoding tools for monosynpatic retrograde neuronal tracing, to be used with modified rabies...be used with modified rabies virus to perform retrograde neuronal tracing. Note : Addgene does not distribute...deletion-mutant rabies virus AAV1 Wickersham 100799 pAAV-TREtight-mTagBFP2-B19G AAV1 Wickersham Introduction to ..., Finke S, Conzelmann KK, Callaway EM. 2007a. Retrograde neuronal tracing with a deletion-mutant rabies...

...filtering options. *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova... dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Promoter Sensor...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? ...viruses would be helpful to the scientific community. Return to top...

..., or by suggesting plasmids for the collection. Return to top Plasmid Kits for Microbiology Kits are sets...therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression - Pertz Lab...common S. cerevisiae lab strains - Ralser Lab Return to top Resources for Microbiology General CRISPR...Genome Database (SGD) (Link opens in a new window) Return to top Do you have suggestions for other plasmids...

... genome editing logistics - Jose-Antonio Daros. Return to top Empty Backbones The table below highlights... Plasmid Description Promoter Cloning Method PI Return to top Fluorescent Proteins The table below highlights...protein of choice. ID Plasmid Description Promoter PI Return to top Other Resources Addgene Blog Posts RUBY-...Arabidopsis thaliana based on RNA-seq analysis. Return to top Do you have suggestions for other plasmids...

...Structure Plasmids Phamret 458 517 14 Monomer Phamret-N1 - Mammalian Expression Phamret-C1 - Mammalian Expression...Empty Backbones More Fluorescent Protein Resources: FRET Biosensors Subcellular localization Michael Davidson...- Mammalian Expression pMXs-mRFP1 - Mammalian Retroviral Expression mCherry 587 610 16 4.5 15 min Monomer...

...coding sequence ~1 kb shorter than SpCas9 while retaining the same basic function. Its size allows it to...AAV backbones for in vivo experiments and in a retroviral backbone for delivery to cells that are poorly...Cas9 Fusion Tools dCas9 cannot cleave DNA, but it retains the ability to find specific DNA targets through...specificity, and editors carrying an ADAR truncation that retain RNA editing capabilities but are small enough to...23979020 Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W.,...., Terns, M. P., White, M. F., Yakunin, A. F., Garrett, R. A., Van Der Oost, J., . . . Koonin, E. V. (...: 32572269 Charpentier, M., Khedher, A. H. Y., Menoret, S., Brion, A., Lamribet, K., Dardillac, E., Boix...

...plasmids third-generation rtTA On Elledge 19407 pTREtight2 Promoter contains a modified TRE that is silent...Expresses firefly luciferase hairpin and GFP under pTREtight promoter None Either Elledge 35625 pAAV-Ptet-RFP-shR-rtTA... 3rd generation rtTA On Campeau 25434 pMA2640 Retroviral; CMV-driven; linked via IRES to EGFP-Blasticidin...EGFP-Blasticidin fusion; pMA2641 has rtTA driven by retroviral LTR promoters ; see article for lentiviral vectors...

... Optogenetics AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? ...viruses would be helpful to the scientific community. Return to top...

...Plasmids RXRA, NR2B1 retinoid X receptor, alpha RXRG Plasmids RXRG, NR2B3, RXRC retinoid X receptor, gamma... receptor gamma RARA Plasmids RARA, NR1B1, RAR retinoic acid receptor, alpha RORA Plasmids RORA, ROR1,...

...was generated with the help of David Sabatini . Return to top mTOR Pathway - Gene List Click on a name...sclerosis VHL Von Hippel-Lindau tumor suppressor Return to top mTORC2 Complex Symbol Name AKT AKT1 AKT2...complex 2 SGK Serum/glucocorticoid regulated kinase 1 Return to top Resources Cancer Pathway ORF Kit (Sabatini...

... AM Cre and Flpo Katherine Matho 10:15 AM AAV Retrograde Tracing Gowan Tervo and Sarada Viswanathan 11...of research materials to share: In vivo data: Retrograde hSyn1-GCaMP6f-P2A-nls-dTomato Detailed protocol....doi: 10.3389/fnana.2019.00093. PubMed 31849618 Return to top...

...81070 rAAV2-retro helper AAV retrograde AAV packaging plasmid, expressing Rep2 and retrograde capsid Alla...

..., or by suggesting plasmids for the collection. Return to top Plasmid Kits and Collections Kits are sets...synthetic biology plasmids for use in C. elegans . Return to top Other Resources Addgene Blog Posts An “elegans...genome of the genetic model organism C. elegans. Return to top Do you have suggestions for other plasmids...

...AICSDP-41 mEGFP SERCA2 Sarcoplasmic reticulum/endoplasmic reticulum 114406 SMC1A-mEGFP AICSDP-57 mEGFP ...SEC61B-mEGFP AICSDP-7 mEGFP Sec61 beta Endoplasmic reticulum 87427 FBL-mEGFP AICSDP-13 mEGFP Fibrillarin Nucleolus...