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...best-characterized effects of p53 are in promoting cell cycle arrest, apoptosis, or senescence in damaged cells. The... receptor Gadd45 GADD45A GADD45B GADD45G Growth arrest and DNA-damage-inducible; alpha, beta, or gamma...BCL2 binding component 3 Reprimo TP53 dependent G2 arrest mediator candidate Scotin Shisa family member 5...

...plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging plasmid — contains packaging... Any expression cassette (promoter and gene of interest) can be cloned into the plasmid. Garry Nolan 12257...Bicistronic expression of EGFP and the gene of interest. Can also express shRNA (cloning H1-shRNA cassettes...

...plasmids for endogenous tagging of your gene of interest.... design. How to use CRISPR to tag your gene of interest Mendenhall and Myers Tagging System The Eric Mendenhall...plasmids as listed in each row. If your own gene of interest is currently unavailable, you will need to design...

...conditions: The sequence is unique compared to the rest of the genome The target is present immediately ...Publications CRISPR Overview Bacteria have an interesting adaptive immune response that detects and eliminates... Cas enzyme and a gRNA specific to the gene of interest. The genomic target of the gRNA can be any ~20...activity with endogenous cell-type specific miRNAs to restrict CRISPR editing only to the target tissue. Browse... molecules associated with a genomic region of interest in vivo. Figure 12: Overview of purifying genomic...

... molecules associated with a genomic region of interest in vivo is essential to understanding locus function... molecules associated with a genomic region of interest in vivo. Figure 1: Overview of purification of...

...repeat arrays of any desired length: the REAL (Restriction Enzyme And Ligation) assembly method (Sander ...Targeter enables users to search their sequences of interest for potential TALEN or TALE transcriptional activator...

...the system is to infect the cell population of interest with a deletion-mutant tracing virus that lacks..., Young JA, Callaway EM. 2007b. Monosynaptic restriction of transsynaptic tracing from single, genetically...

...plasmids for a wide array of research purposes. Interested in Addgene's viral services? See Addgene's Packaged...these viral vectors are used to transduce cells of interest. Specific modifications to these plasmids, such...

...microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses, protozoa, fungi,... cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae lab strains...

... viral biology without disturbing the virus of interest and monitoring endogenous protein dynamics in ...interaction (PPI) studies, where each protein of interest is fused to either LgBiT or SmBiT and expressed...

...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...vertebrate brain - Alex Schier Lab Other Tools of Interest A few examples of popular tools to use in zebrafish...

...regardless of age or symptom severity, suggesting that restoring MECP2 expression in humans could be curative. ...therapeutic strategies to treat Rett syndrome and restore functional MECP2 in cells. Search (Link opens in...

...variants of LRRK2 , SNCA , and other proteins of interest. Browse or search the table below. ID Plasmid ...

...research applications. Browse Antibodies Production Interested in our production process? At Addgene, we provide...

... efficient when the gRNA targets your gene of interest’s promoter, enhancer, or the beginning of the coding...

...you to create fusion proteins with your gene of interest. Most of these plasmids are available to industry...

...promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also ...

...specific promoter or enhancer for your gene of interest. These modifications persist over time and are...

...Category Empty Backbones Study your protein of interest by fusing it to a fluorescent protein. Michael...

...a component to find plasmids with your gene of interest. Browse Cancer Research Plasmids by Pathway mTOR...