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We narrowed to 65 results for: Rest

Showing: 21 - 40 of 65 results

  1. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...it's important to determine where your protein of interest resides. There are a variety of methods for determining...to compare the localization of your protein of interest with known markers. Addgene has assembled a collection...subcellular structures. You should fuse your protein of interest with a fluorescent protein that has a different...localization or possible function of a protein of interest. Browse or search the table below to find markers...

  2. p53 Pathway

    Type
    Collection
    ...best-characterized effects of p53 are in promoting cell cycle arrest, apoptosis, or senescence in damaged cells. The... receptor Gadd45 GADD45A GADD45B GADD45G Growth arrest and DNA-damage-inducible; alpha, beta, or gamma...BCL2 binding component 3 Reprimo TP53 dependent G2 arrest mediator candidate Scotin Shisa family member 5...

  3. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also ...transcription. Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding...specific promoter or enhancer for your gene of interest. Available modifications include histone acetylation...

  4. Lentivirus Plasmids

    Type
    Collection
    ...encode a wide range of gene products. The main restrictions on a lentiviral transfer plasmid are size (i.e... Any expression cassette (promoter and gene of interest) can be cloned into the plasmid. Nolan 12257 pWPXL...bi-cistronic expression of EGFP and the gene of interest, also can express shRNA (cloning H1-shRNA cassettes...

  5. CRISPR Plasmids - Tagging

    Type
    Collection
    ...plasmids for endogenous tagging of your gene of interest.... design. How to use CRISPR to tag your gene of interest Mendenhall and Myers Tagging System The Eric Mendenhall...plasmids as listed in each row. If your own gene of interest is currently unavailable, you will need to design...

  6. CRISPR Guide

    Type
    Collection
    ...conditions: The sequence is unique compared to the rest of the genome The target is present immediately ...Publications CRISPR Overview Bacteria have an interesting adaptive immune response that detects and eliminates... Cas enzyme and a gRNA specific to the gene of interest. The genomic target of the gRNA can be any ~20...activity with endogenous cell-type specific miRNAs to restrict CRISPR editing only to the target tissue. Browse... molecules associated with a genomic region of interest in vivo. Figure 12: Overview of purifying genomic...

  7. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ... molecules associated with a genomic region of interest in vivo is essential to understanding locus function... molecules associated with a genomic region of interest in vivo. For CRISPR-based purification, design...

  8. Fluorescent Protein Guide

    Type
    Collection
    ...localization and/or function of your protein of interest by fusing it to a fluorescent protein. FRET Find...Subcellular Localization Determine where your protein of interest resides by using a well-characterized fluorescent...

  9. TALEN Engineering

    Type
    Collection
    ...repeat arrays of any desired length: the REAL (Restriction Enzyme And Ligation) assembly method (Sander ...Targeter enables users to search their sequences of interest for potential TALEN or TALE transcriptional activator...

  10. AAV for Neuronal Tracing

    Type
    Collection
    ...the system is to infect the cell population of interest with a deletion-mutant tracing virus that lacks..., Young JA, Callaway EM. 2007b. Monosynaptic restriction of transsynaptic tracing from single, genetically...

  11. CRISPR Plasmids - Yeast

    Type
    Collection
    ...promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also ...transcription. Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding...

  12. Microbiology Resources

    Type
    Collection
    ...microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses, protozoa, fungi,... cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae lab strains...

  13. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also ...transcription. Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding...

  14. Zebrafish Plasmid Collection

    Type
    Collection
    ...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...vertebrate brain - Alex Schier Lab Other Tools of Interest A few examples of popular tools to use in zebrafish...

  15. CRISPR Plasmids - Plants

    Type
    Collection
    ...promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also ...transcription. Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding...

  16. Rett Syndrome

    Type
    Collection
    ...regardless of age or symptom severity, suggesting that restoring MECP2 expression in humans could be curative. ...therapeutic strategies to treat Rett syndrome and restore functional MECP2 in cells. Search (Link opens in...

  17. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...cells that are then screened for a phenotype of interest. Screening experiments using a pooled CRISPR library...individual library page. Find Pooled Libraries of Interest Click on different properties to create a custom...

  18. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...you to create fusion proteins with your gene of interest....you to create fusion proteins with your gene of interest. These are organized By Color of the fluorescent...

  19. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Constitutive or Cre-dependent) Cell-specific restoration of stimulus preference after monocular deprivation...augmented transmembrane chloride movement by gerbil prestin (SLC26a5). PLoS One. 2014 Jun 5;9(6):e99095. Joseph...
Showing: 21 - 40 of 65 results