..., inflammation, metabolism, fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the...

...Toxoplasma (toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium (cryptosporidiosis), Leishmania...Leishmania (leishmaniasis), and others have been the focus of biomedical research for decades. Recent developments...

...Caenorhabditis elegans. Mos1 Based Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit...transparent body of the C. elegans hermaphrodite consists of 959 somatic cells, which makes them attractive...

...applying the wild-type CRISPR system for genome mutagenesis ( Cong et al. , Mali et al. , Jiang et al. , ...dcas9 gene on a p15A vector with chloramphenicol resistance. The second plasmid ( pgRNA_bacteria ) contains...expression cassette on a ColE1 vector with ampicillin resistance, wherein the N20 can be custom designed to target...

...72258 Expresses FLPe, copGFP and the puromycin resistance from the CB promoter pCDH-CB-FLPe-P2A-tdTomato...copGFP-T2A-Puro 72263 Express copGFP and the puromycin resistance gene from the EF1 promoter pCDH-CMV-mCherry-T2A-Puro...-T2A-Puro 72264 Express mCherry and puromycin resistance gene from the CMV promoter pCDH-CMV 72265 Express...

...opens in a new window) The Open Enzyme Collection consists of more than 75 genes for enzymes that are considered...reverse transcription of RNA into DNA, and dNTP synthesis. Additionally, the Open Enzyme collection contains...bacteria. Researchers can use the collection as the basis of manufacturing their own enzymes by cloning them...

...preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver staining or SYPRO...purified and non-purified AAV subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are...preparation shows that the vast majority of the vectors consist of full particles (white arrowhead) relative to...

... Minibodies are engineered antibody fragments consisting of two scFvs bound to a CH3 domain. Fab fragments...specific proteins or protein complexes for downstream analysis. They are powerful tools for understanding protein...complex and can be sequenced and amplified for analysis. Overview of application Process tissue or cell...crosslink, isolate DNA, and perform downstream analysis. Special considerations One type of ChIP assay...

...On System In 1995, Gossen et al. used random mutagenesis to identify which amino acid residues of tetR...lines Tet-On Advanced; Plasmid 26730 has hygro resistance 3rd generation rtTA On Campeau 25434 pMA2640 ...underlying expression of Tn10 encoded tetracycline resistance. Hillen W and Berens C. Annu Rev Microbiol . ...

...off-target effects). Ensure that the guide sequences consist of a 20-mer (“protospacer sequence”) upstream of..., resolve agarose gel to determine if size is consistent with the presence or absence of deletion. For...suspension cell line. The culture medium in all steps consists of DMEM supplemented with 2% penicillin/ streptomycin...

... signal, sensitivity, wide dynamic range, and resistance to photobleaching has made luciferase a common...serves as a convenient toolkit to assess the proteostasis status in a wide range of experimental systems...screening method for the systematic and quantitative analysis of co-immunoprecipitations using both Renilla ...

...promotes human nuclear reprogramming and regulates mitosis. Nat Commun. 2016 Mar 7;7:10869. Hu CRISPRa Human...induced Beta Cells Adenoviral Mouse Long-term persistence and development of induced pancreatic beta cells...iPSCs Bipolar Neurons Lentiviral Human Rapid neurogenesis through transcriptional activation in human ...

...factors for cellular programming or cancer drug resistance....applications such as cellular programming or cancer drug resistance. Protocols & References A transcription factor...

...vectors for in vitro and in vivo research. TP53 Mutagenesis Library : Pooled library from the Hahn and Root...Library (Frank Stegmeier) Tackling Cancers’ Drug Resistance with a New Screening Kit (David Sabatini & Kris...

...1000000013 ) Depositing Labs: Keith Joung This kit consists of plasmids and strains used to engineer ZFNs ...Oligomerized Pool ENgineering (OPEN) method. This kit consists of 5 individually packaged plasmids and 3 individually...

...If any discrepancies are identified during this analysis, you will be notified by email. If Addgene determines... the material received. Shipping While Addgene assists importation by including thorough shipping documents...

...Alzheimer's Disease (AD), Amyotrophic Lateral Sclerosis (ALS), Frontotemporal dementia (FTD), Huntington's...Zena Werb 19228 pBS ALS2 ALS2 T7 Primary lateral sclerosis juvenile Cliff Tabin 19457 pcDNA5/FRT/TO HSPA1L...Parkinson's, Kufor-Rakeb syndrome, Neuronal ceroid lipofuscinosis Veerle Baekelandt 171771 miR3 Mm ATP13A2 ATP13A2...Parkinson's, Kufor-Rakeb syndrome, Neuronal ceroid lipofuscinosis Veerle Baekelandt 171786 MSCV-Fbxo7-IRES-GFP...Parkinson's, Kufor-Rakeb syndrome, Neuronal ceroid lipofuscinosis Veerle Baekelandt 171821 pLenti HsATP13A2 D962N...Parkinson's, Kufor-Rakeb syndrome, Neuronal ceroid lipofuscinosis Veerle Baekelandt 171822 miR5 Mm ATP13A2 ATP13A2...Parkinson's, Kufor-Rakeb syndrome, Neuronal ceroid lipofuscinosis Veerle Baekelandt 171922 pOPINB-hRNF216(457...

...yeast, plants, or insect cells. Benefits include consistency between lots and the ability to optimize the ... vector facilitating production and functional analysis of scFv antibody fragments selected from Tomlinson...

... sp. Bacillus sp. Burkholderia sp. Bordetella pertussis Campylobacter sp. Chlamydia sp. Clostridium sp...Saccharomyces Cerevisiae Archive for Functional Analysis (EUROSCARF) (Link opens in a new window) JGI Integrated...

...lymphoma invasion and metastasis TP53 Tumor protein p53 TSC TSC1 TSC2 Tuberous sclerosis VAV1 Vav guanine ...