... help you get started using CRISPR in your own research. Generating a Knockout Using CRISPR You can use...gene editing for specific gene edits. In 2015, researchers used rational mutagenesis to develop two high...transformed with a pooled library of SpCas9 variants, researchers identified Sniper-Cas , which has less off-target...Double-Strand Breaks To overcome low HDR efficiency, researchers have developed two classes of base editors-- ...particular chromatin and DNA modifications, allowing researchers to isolate the effects of a single epigenetic...once Current multiplex CRISPR systems enable researchers to target anywhere from 2 to 7 genetic loci by...dCas9 fused to a fluorescent marker like GFP, researchers have turned dCas9 into a customizable DNA labeler...

... for distribution to the research community. With these plasmids researchers will be able to generate ...skeletal type Sarcomeric thin filaments 114412 TTN-mEGFP AICSDP-45 mEGFP Titin Sarcomeres (M-line tag) ...mEGFP MLC-2a Sarcomeric thick filaments 114414 MYL2-mEGFP AICSDP-49 mEGFP MLC-2v Sarcomeric thick filaments...Cytoplasm 114405 ATP2A2-mEGFP AICSDP-41 mEGFP SERCA2 Sarcoplasmic reticulum/endoplasmic reticulum 114406 SMC1A-mEGFP...124607 ACTN2-mEGFP AICSDP-63 mEGFP Alpha-actinin-2 Sarcomeric z-disks 124608 NPM1-mTagRFP-T AICSDP-69 mTagRFP-T...

...Laboratory for Cancer Research . Frederick National Laboratory for Cancer Research Color is used for clarity...Laboratory for Cancer Research . Frederick National Laboratory for Cancer Research Return to top Ras Pathway... Cancer Research Ras Pathway Ras Pathway You May Also Like: Ras...Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral oncogene homolog GRB2 Growth factor receptor...exchange factor RAS KRAS MRAS NRAS Kirsten rat sarcoma viral oncogene homolog Muscle RAS oncogene homolog...gene expression vectors for in vitro and in vivo research. The RAS Initiative - at the National Cancer Institute...

...highest-possible quality of research output, RESOLUTE expects to accelerate the pace of research in the field of ...global benefit of basic academic research as well as applied research in small biotech and pharmaceutical...mission is to develop data and reagents to boost the research focused on Solute carrier proteins (SLCs). Browse...mission is to develop data and reagents to boost the research focused on SLCs. Through the coupling of an inclusive...support from the European Union's Horizon 2020 research and innovation programme and EFPIA. Representation...

...synthetic biology collections) for use in C. elegan research....been developed and characterized which allow researchers to express desired constructs in precise cell...highlights plasmids that contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, ...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description...C. elegans strains. CRISPR plasmids from John Calarco's lab as described in Heritable genome editing in...Andrew Fire Lab. A set of vectors for C. elegans research, including lacZ and GFP fusion vectors. C. elegans...computer scientists dedicated to providing the research community with accurate, current, accessible information...

...Elsaesser 154763 pAS_FLAG-G1 PylRS G1 PylRS M. archaeon Mammalian TAG Simon Elsaesser 154764 pAS_FLAG-...154768 pAS_4xG1 PylT FLAG-G1 PylRS G1 PylRS M. archaeon Mammalian TAG Simon Elsaesser 154769 pAS_4xG1 ...4xG1 PylT FLAG-G1 PylRS Y125A G1 PylRS Y125A M. archaeon Mammalian TAG Simon Elsaesser 154773 pAS_4xhybPylT...A41AA C55A FLAG-G1 PylRS Y125A G1 PylRS Y125A M. archaeon Mammalian TAG Simon Elsaesser 154774 pAS_4xU6-... Thomas Huber 174718 pRSF-G1mCNPRS G1mCNPRS M. archaeon L-3-(2-cyano-4-pyridyl)alanine (mCNP) Bacterial... Thomas Huber 174719 pRSF-G1pCNPRS G1pCNPRS M. archaeon L-3-(2-cyano-5-pyridyl)alanine (pCNP) Bacterial... Thomas Huber 177310 pRSF-G1(7FTrp)RS PylRS M. archaeon 7-Fluoro-L-Tryptophan (7FTrp) Bacterial TAG Thomas...

...Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent 5...-Syn-Archon1-KGC-GFP-ER2 Syn Archon1 EGFP Constitutive 8 Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2...Norepinephrine (NE) Sensors GRAB_NE Voltage Reporters Archon Voltron JEDI-2P 5-HT Sensors GRAB_5-HT Promoter...-GFP-ER2] Syn Archon1 EGFP Cre dependent 8 Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST...

... Ginkgo Bioworks COVID-19 Plasmid Collection Researchers developing novel diagnostics, therapeutics, and...important experiments. In an effort to speed up research of SARS-CoV-2, Ginkgo Bioworks is using their ...synthesis capabilities, in collaboration with researchers at Stanford University’s Department of Bioengineering...Bioengineering, to build DNA that will enable researchers to express SARS-CoV-2 viral proteins in several ...protein expression and/or purification. Use the search bar or sort buttons to find SARS-CoV-2 plasmids...Extraction Without A Kit External Resources For more research related resources, such as reagents, data, news...

...Addgene Parkinson's Research from The Michael J Fox Foundation Search Use Addgene's Search to find plasmids... Distribution to Industry Featured Collections Search Plasmids Browse Plasmids Browse Antibodies Ordering...Protein Labeling from New England Biolabs Cancer Research from Sinai Health System Plasmids from the Structural...open, efficient and affordable access to plasmid research tools. Pricing will be indicated on the product...

...Lin Voltage Fluorescent voltage reporters Archon 1 and Archon 2 (Constitutive or Cre-dependent) A robotic...extracellular vs. organelle-localized). Browse or search the tables below highlighting a few popular categories...Genetically Encoded Calcium Indicators. Janelia Research Campus. Online resource. 2020 GENIE Project Calcium...: S2451-9456(16)30163-5. Teresa Alonso , Javier García-Sancho Calcium Large palette of genetically encoded...Nat Methods. 2023 Sep;20(9):1426-1436. Tommaso Patriarchi Norepinephrine GRAB_NE family of GPCR activation-based... Nat Methods. 2022 Feb;19(2):231-241. Tommaso Patriarchi Serotonin Red and green serotonin sensors GRAB...potentials in individual neurons and dendrites (ArcLight) Single action potentials and subthreshold electrical...

...Laboratory Gowan Tervo Janelia Research Campus Sarada Viswanathan Janelia Research Campus Kenton Woodard Penn...techonology in the lab (main issues/factors that researchers should consider when planning experiments). What...application? What are some current obstacles that researchers face right now (e.g., not enough protocols, cost...Experimental protocols or schematics. Examples of research materials to share: In vivo data: Retrograde hSyn1...Laboratory Gowan Tervo and Sarada Viswanathan, Janelia Research Campus Kenton Woodard, Penn Vector Core Intended...

...Human Mali 3rd 1 74 Perturb-seq Guide Barcodes (GBC) 85968 Barcode Human Weissman 3rd N/A N/A Retroviral...Available for knockout, activation, inhibition, and barcoding. CRISPR... Addgene's collection. Library Type Activation Barcode Knockout Inhibition Base Editing Species Human ...smegmatis Green monkey ( C. sabaeus ) Kaposi's Sarcoma-associated Herpes Virus (KSHV) Cow ( B. taurus ...2 40,964 Ingolia lab S cerevisiae CRISPRi v1 – barcodes 181005 161769 Inhibition Yeast Ingolia NA ~6 44,580...

...use in plants. These resources include tools for research using plant systems such as Arabidopsis, wheat...MoClo Plant Parts II and Infrastructure Kit for hierarchical assembly of plant multigene constructs - Johannes...gene of interest for expression in plants. You can search the table for features you are looking for, such...luminescent proteins for expression in plants. You can search the table for features you are looking for, such...molecular biology data for Arabidopsis thaliana research. BAR The Bio-Analytic Resource for Plant Biology...expression in the grasses. OpenPlant Synthetic Biology Research Centre (Link opens in a new window) - The initiative...

...used for research, it is the lentiviral genome that encodes genetic material that the researcher wants delivered...most common and useful types of viruses used in research. Lentivirus can transduce both dividing and non-dividing...available at Addgene have been designed for increased researcher safety. Read our Lentiviral Guide for more information...Plasmids One safety feature of lentivirus used in research is that the components necessary to produce an...Please see our CRISPR Empty gRNA plasmids table and search "lenti". ID Plasmid Generation Description PI 8453...

...Shared Research Tools Labs affiliated with MJFF have also generated a number of Shared Research Tools ...Foundation Plasmid Resource MJFF Plasmids Shared Research Tools Addgene is working with The Michael J. Fox...'s disease plasmid resource for the scientific research community. Michael J. Fox Foundation Plasmids ... SNCA and other proteins of interest. Browse or search the full list below. ID Plasmid Gene/Insert Mutations...

....2022.11.026 . TF ORF barcode PCR for NGS QC and bulk screening (85 KB) TF ORF barcode dial out PCR for 10X...transcription factor isoform has a unique 24 bp barcode that simplifies identification of the transcription... KR, Kirchgatterer PC, Verdine VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh OO... open reading frames. Each isoform is uniquely barcoded with a 24 bp sequence, to simplify identification...

...Disease Research Find deposited plasmids for specific disease research areas. Cancer Research Plasmids...Collection Neuroendocrine Tumor Research Foundation Neurodegeneration Research Collection Parkinson's Disease...

...intruders, antibodies are also extremely useful to researchers in a lab. When an antibody binds tightly to a...name a few. Traditional sources of antibodies for research purposes include animals or hybridoma cells, but...antibodies extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant...expression of antibodies, nanobodies or ScFvs. Use the search bar or sort buttons to find antibody plasmids for...Nanobodies, Sybodies, or ScFVs Secondary antibodies (search for "anti-mouse" or "anti-rabbit") ID Plasmid Description...

...with fairly high specificity. Fifteen years of research has shown that when fused to a nuclease or activation...interview with Adam Bogdanove ), progress on TALe research has been swift. The incentives are high. Since...potential targetable sites approximately every 35bp. Researchers are still determining the importance of context...matter of days. In an open access Nucleic Acids Research article that came out in the spring of 2011, the...labs that continue to hone these tools and the researchers who are learning to wield them. Learn more about...

...gene of interest, please search our full plasmid collection using the search bar at the top of this page...pCS2plus-TDP-43 TARDBP CMV ALS Marco Morsch 162609 pCS2plus-TDP-43 M337V TARDBP CMV ALS Marco Morsch 162611 pCS2plus-mVenus1...CMV ALS Marco Morsch 162612 pCS2plus-mVenus1-155-GGGS-TDP43 M337V TARDBP mVenus CMV ALS Marco Morsch 162614... ALS Marco Morsch 162615 pCS2plus-mVenus1-155-I152L-GGGS-TDP43 M337V TARDBP mVenus CMV ALS Marco Morsch...Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia...large collection of neurodegeneration plasmids for research related to Alzheimer's Disease (AD), Amyotrophic...John Gearhart 44333 pMX_mTBP TBP LTR Parkinson's Marc Timmers 44978 GST-FUS-WT FUS GST tac ALS Robin Reed...