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We narrowed to 50 results for: crispr cas9

Showing: 21 - 40 of 50 results

  1. CRISPR Plasmids - Mammalian Expression

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base... to immunoprecipitate FLAG-tagged Cas9. In the CAPTURE system, Cas9 is tagged with a biotin acceptor site... Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of genomic DNA...Publication Visualize A catalytically inactive Cas9 (dCas9) fused to a fluorescent protein (FP) can help...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR... CRISPR Mammalian Expression CRISPR...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...

  2. Brzezinski Lab CRISPR Collection

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    ...for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids...Collections Brzezinski Lab CRISPR Collection Brzezinski Lab CRISPR Collection The Brzezinski lab investigates...involved in retinal development the lab utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate...Find CRISPR plasmids to study the regulatory networks involved in retinal development. Depositor...taken advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several... plasmids dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion...175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express dCas9-VPR for activation...

  3. CRISPR Plasmids - Plants

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Plants CRISPR Plasmids: ...following CRISPR plasmids have been designed for use in plants. Cut Fully functional CRISPR/Cas enzymes... Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI 46968 pICSL01009::AtU6p aU6...CRISPR expression plasmids that have been designed for use in plants. CRISPR...: Plants Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...

  4. CRISPR Plasmids - Cascade-Cas3

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR...Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... Cas3 is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates single-strand...genomic deletions. Compared to deletions generated by Cas9, large deletions created using Cas3 have fewer off-target...break, either due to a boundary defined by anti-CRISPR proteins or running into other proteins bound to...

  5. CRISPR Plasmids - gRNAs

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    Collection
    ... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids By Function Genome...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...PAM sequence. Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments...species or PAM binding variant of Cas9. For instance, wild-type SpCas9 must be used with targets that are...increases off-target effect. Which species or variant of Cas9 ( S. pyogenes , S. aureus etc.) was this gRNA sequence...experiments use targeting sequences within exons, whereas CRISPR activation or repression experiments use targets...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity...

  6. CRISPR Plasmids - Purify Genomic Loci

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying... CRISPR Purify CRISPR: Purify Genomic ... for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved by fusing a biotin...interest in vivo. For CRISPR-based purification, design your gRNA sequence to direct dCas9 to a specific locus...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...essential to understanding locus function. Using CRISPR, researchers have expanded chromatin immunoprecipitation...

  7. CRISPR Plasmids - Parasites

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic... CRISPR Parasites CRISPR Plasmids...research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and ... Libraries Toxoplasma CRISPR Knockout Pooled Library - Toxoplasma gondii CRISPR genome-wide knockout gRNA...forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for...CRISPR expression plasmids that have been designed for use in parasites. CRISPR...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

  8. CRISPR Plasmids - RNA Targeting

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR... Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. In ...crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require that each DNA target have... of Cas enzymes? CasPEDIA is an encyclopedia of CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR RNA targeting plasmids. Plasmids are available ...

  9. CRISPR Plasmids for Genomic Visualization

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Using... CRISPR Visualize CRISPR Plasmids:... inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have turned dCas9 into a customizable...CRISPR/Cas plasmids for labeling and visualization of genomic loci. CRISPR...Plasmids: Visualize Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...fluorescent proteins to visualize targeted genomic loci. CRISPR imaging has numerous advantages over other imaging...like fluorescence in situ hybridization (FISH), CRISPR imaging offers a unique method for detecting the...

  10. Validated gRNA Sequences

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    ...Sequences You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The table below...PAM sequence). Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments...described on Addgene's CRISPR Plasmids and Resources page have been used to indicate the Cas9 application the...single species or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must be used with targets that are upstream... Genome Engineering CRISPR Validated gRNA Sequence Datatable Validated gRNA...that have been experimentally validated for use in CRISPR experiments. This datatable is compiled from plasmids...activity. Know your target. Which species or variant of Cas9 ( S. pyogenes, S. aureus etc.) was this gRNA sequence...

  11. Neurodegeneration Research Collection

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    ...Dhekne et al. Elife. 2023 Oct 24. See More CRISPR Tools Find CRISPR pooled libraries available from Addgene...Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent rodent...neurodegeneration research. Disease Info Plasmid Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors...about the blood brain barrier integrity, and using CRISPR screens to understand more globally how neurons...species. Boender et al. Sci Adv. 2023 Jun 2. Use Cas9 in astrocytes. Endo et al Science. 2022 Nov 4. Base...plasmid pooled libraries . New and Noteworthy: Use a CRISPRi system to target alpha-synuclein. Sastre et al....

  12. AAV Molecular Tools

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    ...Engineering Tools These AAV encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome editing...AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA CMV-driven, constitutive Expression of human codon-optimized Cas9 from Staphylococcus...Staphylococcus aureus (SaCas9). 6, 8 Feng Zhang Labeling Tools These AAV encode tools that can be used...

  13. CRISPR Pooled gRNA Libraries

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is ... CRISPR Pooled Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function...Human CRISPRi Non-coding Libraries 86538 — 86550 Inhibition Human Weissman 3rd 10 Varies CRISPR/Cas9-assisted...experiments using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic...steps for using pooled CRISPR libraries and keep the following in mind: CRISPR screening experiments require...primary cells). Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells...Interferon-Stimulated Gene CRISPR Knockout Library 125753 Knockout Human Emerman OhAinle 3rd 8 15,416 Human CRISPR lncRNA ...

  14. Distribution to Industry

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    ...Mutation Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation...Fusions from Promega COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson...

  16. Rett Syndrome

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    ...Addgene's CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR resources...'s Plasmids for Stem Cell Research page. CRISPR Tools CRISPR technology is a promising therapeutic approach...plasmids expressing Cas9 and empty gRNA backbones. A few examples of additional CRISPR resources can be ...Resource Center MECP2 Animal Models Human Cell Lines CRISPR Tools Plasmids Resources The (Link opens in a new...be found below: Base Editing RNA Editing CRISPR-based DNA methylation editing system - Shawn Liu and Rudolf...

  17. Cre-Lox and Other Site-Specific Recombinases

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    ...Tumor Suppressor Gene CRISPR Knockout Library CRISPR Sidi Chen Use this AAV CRISPR knockout library expressing...vivo using a mouse line expressing Cre-dependent Cas9. Kits Kit Name Type PI Description dRMCE Plasmid...Plasmid Collection Monosynaptic Neuronal Tracing CRISPR Plasmids and Resources Content last reviewed on...

  18. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ...Representative Empty Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector ...for RNA-guided genome editing in plants Visit our CRISPR Plasmids and Resources collection, and browse our... Separate expression pRGEB31 - Deliver sgRNA and Cas9 into plants by Agrobacterium-mediated transformation...Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated... FP Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions... Lentiviral shRNA expression lentiCRISPRv2 hygro - Variant of lentiCRISPR v2 that confers hygromycin resistance...

  19. Bacterial Expression Systems

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    ...tools like CRISPR-Cas9 are not only valuable but often essential. Find a collection of CRISPR plasmids ...with Addgene's CRISPR Guide . Browse CRISPR plasmids available at Addgene through our CRISPR Plasmids and...Bacterial CRISPR Plasmids page . Additional Addgene Genome Engineering Resources Learn more about CRISPR with...zinc finger proteins. Addgene Blog CRISPR Methods for Bacteria CRISPR-based Genome Editing Systems For ...Empty Backbones Collection Molecular Biology Guide CRISPR Guide Bacteria are commonly used to create, store...coli INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Return to top Content last reviewed...Plasmid Promoter Inducer Expression Species PI 44249 pdCas9-bacteria pTetO Anhydrotetracycline (aTc) Escherichia...

  20. CRISPR Plasmids and Resources

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    ...Xenopus. Addgene CRISPR Resources CRISPR Guide : Essential background information on CRISPR and the basics...planning your first CRISPR experiment. CRISPR 101 eBook : Based on our popular CRISPR 101 blog series, we've...analysis, discover links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial...genome engineering. CRISPR Topic Page : Want the latest news on CRISPR? Experts cover CRISPR topics on Addgene's... are used together in CRISPR experiments. CRISPR Pooled Libraries : Find CRISPR libraries for knockout...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR... information you need to use CRISPR in your own lab. Check out our CRISPR resources or browse plasmids...
Showing: 21 - 40 of 50 results