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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 47 results for: nsf

Showing: 21 - 40 of 47 results

  1. Promega Plasmid Collection

    Type
    Collection
    ... educational resources, and efficient material transfer solutions that help advance science while reducing...Promega collection are available in two formats: pretransformed in bacteria as an agar stab 20 µg purified ...NanoBRET (Link opens in a new window) is an energy transfer technique designed to measure molecular proximity...

  2. Viral Production

    Type
    Collection
    ...of Pennsylvania Vector Core. Transfections are performed using the transfer plasmid, a plasmid encoding... taken off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... Cas9 into plants by Agrobacterium-mediated transformation Dual-sgRNA_hU6-mU6 - Lentiviral expression ...Tet-inducible lentiviral shRNA expression Find yeast transformation plasmids with G418 selection Find nematode ...ubiquitin promoter for Agrobacterium-mediated transformation Blasticidin Mammalian pLX304 - 3rd gen lentiviral...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ...human codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols... #61422 - #61424 were designed for transient transfection : 61422 : Expresses dCAS9-VP64 activator with...codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar to ...

  5. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...protein (cpFP) or fluorescence resonance energy transfer (FRET) efficiency of a pair of fluorescent proteins...in plant cells Photosynthesis-dependent H 2 O 2 transfer from chloroplasts to nuclei provides a high-light...metabolism (FLIPmal) Fluorescence Resonance Energy Transfer sensors for quantitative monitoring of pentose...temperature indicator B-gTEMP Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature...

  6. Brain Initiative Collection

    Type
    Collection
    ...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s...Synapsin1 promoter, for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2...

  7. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...different cell types. Zinc finger arrays can be transferred in-frame to these vectors....either OPEN or modular assembly can be easily transferred in-frame to any of these vectors using unique...

  8. DNA Service - Cloning Grade DNA

    Type
    Collection
    ... different DNA concentrations. Can I directly transfect cloning grade DNA into cells? Cloning grade DNA...designed for use in cloning applications. Direct transfection into cells is not recommended. Can I order more...

  9. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...generation R-mAbs plasmids, production of R-mAbs in transfected cells and their validation can be found below...inexpensively generate R-mAbs by transiently transfecting the plasmids into mammalian tissue culture cells... plasmids can be used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target Target...following plasmids can be used to produce scFvs from transfected mammalian cells. Addgene ID Plasmid Hybridoma...plasmids can be used to produce nanobodies from transfected bacterial cells. Addgene ID Plasmid Hybridoma... recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells....

  10. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ... vectors from the Penn Vector Core have been transferred to Addgene for distribution. Look up the IDs ...actual material being referenced in the Material Transfer Agreement (MTA) and its inclusion is what gives...

  11. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...(see paper) Transfection none, need Cas9 plasmid Calarco 47943 pMB70 cU6 BsaI Transfection none, need ...

  12. Adenovirus Plasmids

    Type
    Collection
    ...both plasmids. This DNA molecule can then be transfected into mammalian packaging cell lines to generate...of interest? Search our database for adenoviral transfer plasmids that have inserts. ID Plasmid Type Description...

  13. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Microtubules 101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase 1 Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP ...human iPS cells (Link opens in a new window) RNP transfection for gene editing human iPS cells (Link opens...

  14. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...Addgene AAV transfer plasmids...viral genome (typically called cis -plasmid or transfer plasmid). Helper Plasmid ID Plasmid Serotype Description...

  15. CRISPR Guide

    Type
    Collection
    ...libraries are a heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains ...then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector...short 3′ overhang, allowing for directional gene transfer, which may increase the efficiency of gene editing... Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers...

  16. Bacterial Expression Systems

    Type
    Collection
    ... Protein Interactions Förster resonance energy transfer (FRET) and bimolecular fluorescence complementation...that when a donor fluorophore is excited, it can transfer its energy to an acceptor fluorophore if they ...

  17. Antibody Guide

    Type
    Collection
    ...hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express antibodies.... an SDS-PAGE gel to separate proteins by size. Transfer proteins from the gel to a membrane. Incubate ...

  18. AAV Viral Preps

    Type
    Collection
    ... Packaged on Request service. You can choose a transfer plasmid from our repository and request packaging...

  19. Mammalian RNAi Tools

    Type
    Collection
    ...short interfering RNA). Delivery can occur via transfection or viral delivery of a plasmid. shRNAs are often...

  20. Synthetic Biology - Overview

    Type
    Collection
    ...Negative Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard...
Showing: 21 - 40 of 47 results