We narrowed to 45 results for: nsf

...of Pennsylvania Vector Core. Transfections are performed using the transfer plasmid, a plasmid encoding... taken off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching...

... Cas9 into plants by Agrobacterium-mediated transformation Dual-sgRNA_hU6-mU6 - Lentiviral expression ...Tet-inducible lentiviral shRNA expression Find yeast transformation plasmids with G418 selection Find nematode ...ubiquitin promoter for Agrobacterium-mediated transformation Blasticidin Mammalian pLX304 - 3rd gen lentiviral...

...human codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols... #61422 - #61424 were designed for transient transfection : 61422 : Expresses dCAS9-VP64 activator with...codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar to ...

...protein (cpFP) or fluorescence resonance energy transfer (FRET) efficiency of a pair of fluorescent proteins...in plant cells Photosynthesis-dependent H 2 O 2 transfer from chloroplasts to nuclei provides a high-light...metabolism (FLIPmal) Fluorescence Resonance Energy Transfer sensors for quantitative monitoring of pentose...temperature indicator B-gTEMP Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature...

...different cell types. Zinc finger arrays can be transferred in-frame to these vectors....either OPEN or modular assembly can be easily transferred in-frame to any of these vectors using unique...

...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s...Synapsin1 promoter, for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2...

... different DNA concentrations. Can I directly transfect cloning grade DNA into cells? Cloning grade DNA...designed for use in cloning applications. Direct transfection into cells is not recommended. Can I order more...

... vectors from the Penn Vector Core have been transferred to Addgene for distribution. Look up the IDs ...actual material being referenced in the Material Transfer Agreement (MTA) and its inclusion is what gives...

...need to use two plasmids: A shuttle plasmid (or transfer plasmid) containing the transgene of interest,...Addgene's Biosafety Resources Protocols General Transfection Bert Vogelstein’s lab practical guide for using...

...generation R-mAbs plasmids, production of R-mAbs in transfected cells and their validation can be found below...inexpensively generate R-mAbs by transiently transfecting the plasmids into mammalian tissue culture cells... plasmids can be used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target Target...following plasmids can be used to produce scFvs from transfected mammalian cells. Addgene ID Plasmid Hybridoma...plasmids can be used to produce nanobodies from transfected bacterial cells. Addgene ID Plasmid Hybridoma... recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells....

...Microtubules 101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase 1 Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP ...human iPS cells (Link opens in a new window) RNP transfection for gene editing human iPS cells (Link opens...

...libraries are a heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains ...then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector...short 3′ overhang, allowing for directional gene transfer, which may increase the efficiency of gene editing... Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers...

... Protein Interactions Förster resonance energy transfer (FRET) and bimolecular fluorescence complementation...that when a donor fluorophore is excited, it can transfer its energy to an acceptor fluorophore if they ...

...hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express antibodies.... an SDS-PAGE gel to separate proteins by size. Transfer proteins from the gel to a membrane. Incubate ...

... Packaged on Request service. You can choose a transfer plasmid from our repository and request packaging...

...short interfering RNA). Delivery can occur via transfection or viral delivery of a plasmid. shRNAs are often...

...Negative Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard...

... is based on the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein, and is suitable...

...Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells, the R-mAbs...

...Differentiation Factors Find plasmids used to transform iPSCs and fibroblasts into neurons and more. Convert...