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Addgene

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We narrowed to 40 results for: organization Addgene

Showing: 21 - 40 of 40 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ...gene expression Lentiviral backbones made by Addgene Addgene has an extensive collection of plasmids for...A selection of Addgene's empty vector backbones curated by species, epitope tags/fusion proteins, function...to consider. Here is a guide to a selection of Addgene's empty vector backbones. For the most part, we ...promoter that will be functional in your host organism. Host Relevant Promoters Representative Empty ..., or zebrafish expression GST plasmids made by Addgene MBP Protein purification pET28-MBP-TEV - Bacterial...efficiency, but do NOT integrate into the host genome Addgene has an extensive collection of plasmids for packaging... shRNA expression Retroviral backbones made by Addgene Adenoviral High transduction efficiency, but do...

  2. AAV Packaged on Request

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    Collection
    ...experiments as soon as they receive their AAV prep from Addgene — eliminating the steps to produce, purify, and...this service. Select any eligible plasmid in the Addgene repository in your choice of: serotype (AAV1, AAV2...production Density centrifugation purification Addgene’s comprehensive quality control process: Titering...Approval 1+ business days, varies by requesting organization Viral vector preps remain subject to the terms...remember to share your protocols and results through Addgene’s Data Hub . By contributing your AAV vector experience...let you know whether the item can be packaged. Addgene does not perform functional or in vivo testing.... we produce high-quality viral vector preps at Addgene, including purification, titration, and quality...

  3. mTOR Pathway

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    Collection
    ...Addgene's plasmids for the mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates...mTORC2 influences cell survival and cytoskeletal organization. Cancer Impact Enhanced signaling through either...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  4. Viral Vectors

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    Collection
    ...List of viral vector plasmids available at Addgene and other related resources. ...Viral Plasmids and Resources You May Also Like... Addgene's Viral Service Biosafety Guide Blog: Plasmids 101... for select plasmids are now available through Addgene, learn more here . Browse Viral Plasmids by Type... this genetic material to specific cells in an organism. Figure 1. Using viral plasmids to generate virus...general virus biosafety guidelines, check out Addgene's Biosafety Resource Guide . References Cohen S,...

  5. CRISPR Pooled gRNA Libraries

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    Collection
    ...particular organism, and the library is only compatible with cells derived from that organism. All of our...pick a library. The pooled CRISPR libraries in Addgene’s collection differ based on some important criteria...filtered list of the CRISPR pooled libraries in Addgene's collection. Library Type Activation Barcode Knockout...

  6. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...Addgene's collection of plasmids suitable for FRET experiments to measure protein interactions or conformation... not required to be fluorescent. A variety of organic dyes and fluorescent proteins have been developed... molecules in either of these types of studies Addgene has a collection of plasmids suitable for creating...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  7. TALEN Plasmids and Kits

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    Collection
    ...plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE plasmids.... TALEN Plasmids and Kits You may also like... Addgene's Genome Engineering Guide CRISPR Plasmids Zinc ...amino acid repeats in the TAL effectors. View Addgene's TALEN Guide. These collections and accompanying...Golden Gate cloning method. Validated in multiple organisms. Dan Voytas Adam Bogdanove Joung Lab TAL Effector...Takashi Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  8. Caltech Systemic Capsids

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    Collection
    ...Ready-to-use AAV available from Addgene's viral service. Engineered systemic constructs from Viviana ...As part of our Viral Vector Packaging Service , Addgene is distributing ready-to-use viral preparations...target cell type or tissue of interest, and model organism under study. *Many engineered AAVs for mice use...depth descriptions of each serotype, check out Addgene's blog on Systemic Capsids . To review performance...data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER Center’s repository...produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005) . Note on PHP.eB tropism and specific ... produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006) . Browse Available PHP.S AAV ID Name Promoter...

  9. Luciferase Plasmid Collection

    Type
    Collection
    ...Browse Addgene's collection of luciferase plasmids for assays of transcriptional regulation and bioluminescent... bioluminescence found in a diverse number of organisms, ranging from bacteria to aquatic animals to insects... The most common types of luciferase found in Addgene’s collection are Firefly luciferase (Fluc), Renilla...interest before or after luciferase . In addition, Addgene has many luciferase expression constructs available...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  10. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...loci can be easily expressed in the same cell or organism. The ability to precisely target CRISPR to a given...expert to see results! Getting Started with CRISPR Addgene has empowered researchers to further develop the...applications. For more information on CRISPR, check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR... CRISPR plasmids for your research. Addgene has CRISPR plasmids for the following models: Mammalian systems...

  11. Plasmids for Stem Cell Research

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    Collection
    ...Browse Addgene's collection of plasmids for the generation and study of stem cells, from iPSC generation...and have the capacity to give rise to an entire organism. iPSC technology removes the possible ethical ...may be difficult to decide which method to use. Addgene's blog post Delivery Methods for Generating iPSCs...know which method you would like to use, browse Addgene's collection of Reprogramming Plasmids in the table...cells or fully differentiated cell types. Browse Addgene's collection of Differentiation Plasmids below. ...inherent disadvantage of iPS cell technology. Browse Addgene's collection of Transdifferentiation Plasmids below...below will help you find plasmids available from Addgene that can be used to create iPSCs or another cell...

  12. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... structures give the cell its shape, organize cellular organelles, and are necessary for movement and ...of your protein of interest with known markers. Addgene has assembled a collection of fluorescent fusion... Plasmid Collections Fluorescent Proteins Organelle Markers Fluorescent Proteins: Subcellular Localization...fusion proteins that are targeted to various organelles or subcellular structures. You should fuse your protein...General Structures The cell contains a variety of organelles and subcellular structures each with their own...table below to find markers of general cellular organelles and structures. ID Plasmid Structure Targeting...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  13. Genetic Code Expansion

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    Collection
    ...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian...pairs of synthetase and tRNA that work for one organism may not work for another. The orthogonal synthetase...

  14. CRISPR Plasmids - Empty gRNA Vectors

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    Collection
    ...Alternatively, gRNA-only plasmids can be used in cells or organisms in which Cas9 has been integrated into the genome...Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your system, you can...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  15. CRISPR Guide

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    Collection
    ...window) . Addgene Website & Blog References Website CRISPR plasmids and resources at Addgene CRISPR 101...cell lines Viral service at Addgene Viral plasmids and resources at Addgene Blog CRISPR topic page How ...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have...You Use a CRISPR Library? CRISPR libraries from Addgene are available in two formats: as liquid DNA, or...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...located. The majority of the CRISPR plasmids in Addgene’s collection are from S. pyogenes unless otherwise...

  16. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...specifically localize to cellular compartments or organelles. Addgene’s database includes many fluorescent biosensors...Cell Physiology Browse All You may also like... Addgene Blog: Biosensors Luciferase Plasmids Subcellular...biosensors can be introduced into cells, tissues, or organisms for long-term imaging and can be designed to specifically...Scaffold for inorganic phosphate biosensor Development of a Reagentless Biosensor for Inorganic Phosphate.... in vivo , cytoplasmic vs. extracellular vs. organelle-localized). Browse or search the tables below ...and In Vivo Imaging of Ca Dynamics in High-Ca Organelles. Cell Chem Biol. 2016 Jun 1. pii: S2451-9456(...sensors (green, red or blue/green) Imaging intraorganellar Ca(2+) at subcellular resolution using CEPIA...

  17. NETRF

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    Collection
    ...Addgene Depositors that have received funding from the Neuroendocrine Tumor Research Foundation...Neuroendocrine Tumors Using Adult Stem Cell-Derived Organoids Matthew Meyerson Dana-Farber Cancer Institute ...

  18. Antibody Guide

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    Collection
    ...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production...applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...intensive. Ready to put your new knowledge to use? Addgene now makes ready-to-use recombinant antibodies made...myeloma cells (immortalized B cells). The genetic reorganization that creates variety in the antibody allowing...the variable region goes through a randomized reorganization process, creating a unique binding site. This...

  19. Immunology Research Plasmids and Resources

    Type
    Collection
    ...Addgene’s immunology collection contains plasmids for genes involved in innate and adaptive immune signaling...Plasmid Tables Additional Resources Mammalian organisms are exposed to millions of potential pathogens...presenting cells. B cells in secondary lymphoid organs recognize specific antigens through the B cell ... 25 cellular signaling pathways and processes. Addgene Blogs Adeno Associated Virus (AAV) for Cell and...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  20. Adenovirus Plasmids

    Type
    Collection
    ...adenovirus plasmids in either mammalian cells or microorganisms, including bacteria and yeast. Two plasmids...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...
Showing: 21 - 40 of 40 results