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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 34 results for: prime editing

Showing: 21 - 34 of 34 results

  1. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...homology to the DNA flanking the DSB and 2. a specific edit close to the gRNA PAM site. When a repair template...

  2. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...

  3. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...

  4. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...

  5. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Activation Barcode Knockout Inhibition Base Editing Prime Editing Other Species Human Mouse Fly Yeast T. gondii... 227706 227707 227708 Prime Editing Human Human Mouse Adamson 3rd 1-20 per edit Varies CRASP-Seq Gene ...3rd ~7 11,364 Dow Lab Base Editing Sensor Libraries 179217-179219 Base Editing Human, Mouse Dow 3rd ~100...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...46,354 BARBEKO sgRNA Library 174163 Knockout, Base Editing Human Wei 3rd 3 53,502 sgRNA Pool for Streptococcus...95,893 CRASP-Seq BE Tiling Library 232070 Base Editing Human Gonatopoulos-Pournatzis 3rd Varies 8,594 ...

  6. CRISPR Plasmids and Resources

    Type
    Collection
    ...information into a specific DNA site. Base Edit Base editors precisely edit DNA without promoting DSB formation...nickase enzymes generate single-strand DNA breaks. Prime Edit Cas nickase enzymes fused to reverse transcriptase...across multiple systems. RNA Editing Cas13 enzymes fused to deaminases can edit bases in RNA. Other Applications...

  7. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...homology to the DNA flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template...

  8. CRISPR Plasmids - Yeast

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...strand) within a small editing window specified by the gRNA. Adenine base editors convert adenine to inosine...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...homology to the DNA flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template...Insert Promoter Selectable Marker PI Publication Base Edit Catalytically dead dCas9 fused to a cytidine deaminase...used with a repair template to introduce specific edits via homology-directed repair (HDR). ID Plasmid Description...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C...

  9. Plant Plasmids and Resources

    Type
    Collection
    ...multiplex genome editing in monocot and dicot plants - Yao-Guang Liu. Plasmids for prime editing in plants (...biocontainable RNA virus vectors for non-transgenic genome editing across crop species and genotypes - Zhenghe Li....TRV) that simplifies gene silencing and genome editing logistics - Jose-Antonio Daros. Return to top Empty...for Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in...Posts RUBY-Red Siliques CRISPR-mediated Plant Base Editors Save Time with Transient Plant Leaf Transformations...

  10. CRISPR References and Information

    Type
    Collection
    ...CRISPR Edits) This open access tool from Synthego allows you to determine rates of CRISPR editing at a ...your locus of interest and correspond to edited and non-edited cell populations. For more information, ...A webtool that uses deep learning models for prime editor pegRNA design and efficiency prediction. Developed...off-target evaluation for base edits using adenine (ABE) and cytosine (CBE) base editors. Includes a wide range...computational pipeline for the analysis of genome editing experiments. It enables rapid and intuitive interpretation..., Cas12a) and noncleaving nucleases (e.g. base editing). Developed by the Luca Pinello Lab (Link opens...Developed by the Hyongbum Kim Lab . GenET (Genome Editing Toolkit) (Link opens in a new window) A library...

  11. CRISPR Plasmids - Tagging

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...footprints, and can be scaled up for systematic editing of multiple genes. Plasmids can be found associated...

  12. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate...Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI...Ventura A CRISPR/Cas9 toolkit for multiplex genome editing in plants Plant Plasmids for six gRNA module vectors... Assembly. Chen Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing ...for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants Plant PCR-based procedure...CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation Plant Three types...

  13. COVID-19 Resources

    Type
    Collection
    ... is the priming of the S protein by host cell proteases. The S protein of SARS-CoV-2 is primed by the ...viruses. ( 1 ) TMPRSS2 - a serine protease that primes the SARS-CoV-2 S protein and is involved in virus...the serine protease TMPRSS2. Image credit: Maya Kostman for the Innovative Genomics Institute Addgene COVID...

  14. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...is an inexpensive, facile, and efficient genome editing tool that allows genetic perturbation of genes ...robust plasmid delivery before attempting genome editing. Immediately transfer solution from cuvette into...37 °C for 24 - 72 hr. 30 °C may enhance genome editing efficiency, but 37 °C is acceptable. Fluorescence...Table 4 ). Design Deletion Screening Primers Design one set of primers internal to the sequence to be deleted...promoter forward primer: CGTAACTTGAAAGTATTTCGATTTCTTGGC. This is a representative sequencing primer; other flanking...10 μl 2x PCR mix, 0.5 μl forward primer (10 μM), 0.5 μl reverse primer (10 μM), 50-100 ng gDNA, and H 2...deleted (“non-deletion band”) and another set of primers upstream and downstream of the sgRNA cleavage sites...
Showing: 21 - 34 of 34 results