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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 78 results for: sequence

Showing: 21 - 40 of 78 results

  1. Antibody Production

    Type
    Collection
    ... purified, sequenced via the Sanger method, and aligned with the parental plasmid sequence to confirm ...protein size is confirmed. Residual Plasmid Sanger Sequencing Purified recombinant antibody preps contain low...

  2. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...immunoprecipitation (ChIP) to allow purification of any genomic sequence specified by a particular gRNA. In the enChIP ...For CRISPR-based purification, design your gRNA sequence to direct dCas9 to a specific locus, avoiding ...

  3. TALEN Engineering

    Type
    Collection
    ...produced by all three methods are identical in sequence. TAL effector repeat arrays produced on this framework... ZiFiT Targeter enables users to search their sequences of interest for potential TALEN or TALE transcriptional...

  4. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...Mismatches between the guide RNA and the target sequence lower the repression level. By controlling the...motifs (squares), and also carrying the tracrRNA sequence (not shown). (B) Relative GFP and RFP concentration...

  5. TALEN Guide

    Type
    Collection
    ...other useful protein domains to a specific DNA sequence ( Figure 1A ). Scientists have identified a large...incentives are high. Since ZF targets are confined to sequences composed of triplets with corresponding zinc fingers...Pubmed PMID: 21960622 . Efficient construction of sequence-specific TAL effectors for modulating mammalian...

  6. Zebrafish Plasmid Collection

    Type
    Collection
    ... for plasmids that contain a zebrafish gene or sequence . The table below highlights plasmids that contain...Zebrafish Genome Project - Access the latest reference sequence assembly for the zebrafish genome. Zebrafish Model...zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been experimentally validated in zebrafish...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ...of target sequence in the 3' UTR David Bartel 12179 pIS1 Renilla Insertion of target sequence in the 3'... Bartel 12177 pIS2 Renilla Insertion of target sequence in the 3' UTR David Bartel 101139 pCMV-IRES-Renilla... PCD FL0X Firefly Insertion of imperfect miRNA sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly...psi-CHECK3 Firefly, Renilla Insertion of 3' UTR sequences downstream of Renilla luciferase. Firefly luciferase...

  8. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...

  9. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ... the following reasons: Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer...Our final step of the QC process, viral genome sequence verification, may not yet have been completed ...

  10. CRISPR Plasmids - Plants

    Type
    Collection
    ...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...promoter/enhancer or the beginning of the coding sequence. If the plasmid you’re using does not also express...

  11. Mammalian RNAi Tools

    Type
    Collection
    ...double-stranded RNA (dsRNA) that matches the mRNA target sequence. The dsRNA can be delivered as shRNA (short hairpin...remaining single-stranded siRNA then binds the target sequence and RISC cleaves and degrades the mRNA. RISC includes...

  12. COVID-19 Resources

    Type
    Collection
    ... (SARS classic) sequences. Mammalian Targets : Plasmids containing mammalian sequences linked to SARS-...available or coming soon containing SARS-CoV-2 sequences. SARS-CoV Plasmids : Plasmids that are available... Plasmids Many plasmids containing SARS-CoV-2 sequences are now available and more are currently undergoing...

  13. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...

  14. Neurodegeneration Research Collection

    Type
    Collection
    ...abnormally long polyglutamine sequences. These glutamine-rich sequences are prone to misfolding and aggregation...neuroscience research created with high-volume hybridoma sequencing on the NeuroMabSeq platform. Mitchell et al. ...

  15. Deisseroth INTRSECT Collection

    Type
    Collection
    ...orthogonal recombinase (Cre and Flp) recognition sequences within synthetic introns. INTRSECT was first shown...: INTRSECT works by inserting short, intronic sequences into the open reading frame (ORF) of a molecular...molecular tool and adding recombinase recognition sequences (e.g. Lox sites, FRT sites) inside of the introns...The addition of these recombinase recognition sequences enables directional control of the portion of ...

  16. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... pegRNA directs the Cas9 nickase to the target sequence where it nicks the non-target strand and generates..., if the edited 3’ flap is excised, the target sequence remains unchanged and available as a substrate...

  17. Lentiviral Prep Service

    Type
    Collection
    ...by an RNA guide (gRNA) that harbors targeting sequences. Browse our repository for a list of gRNA plasmids...plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used...

  18. AAV Packaged on Request

    Type
    Collection
    ...assessment, sterility testing, and viral genome sequencing....Purity assessment Sterility testing Viral genome sequencing (VGS) Find detailed pricing for your chosen plasmid...

  19. Tetracycline Inducible Expression

    Type
    Collection
    ...protein (TetR) and operator ( tet O, a 19 nucleotide sequence, TCCCTATCAGTGATAGAGA). In normal conditions, the...called Ptet) have been designed by combining tet O sequences with elements like the H1 RNA promoter. And, most...or other species by codon optimization of their sequence and eliminating interactions with endogenous factors...Bujard, H., & Hillen, W. (2000). Exploring the sequence space for tetracycline-dependent transcriptional...

  20. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...generates a double-strand break (DSB) at a specific sequence. Two unique gRNAs, binding ~15-25 bp apart, are...
Showing: 21 - 40 of 78 results