We narrowed to 64 results for: sequence

... the following reasons: Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer...Our final step of the QC process, viral genome sequence verification, may not yet have been completed ...

...double-stranded RNA (dsRNA) that matches the mRNA target sequence. The dsRNA can be delivered as shRNA (short hairpin...remaining single-stranded siRNA then binds the target sequence and RISC cleaves and degrades the mRNA. RISC includes...

...abnormally long polyglutamine sequences. These glutamine-rich sequences are prone to misfolding and aggregation...neuroscience research created with high-volume hybridoma sequencing on the NeuroMabSeq platform. (Mitchell et al....

...orthogonal recombinase (Cre and Flp) recognition sequences within synthetic introns. INTRSECT was first shown...: INTRSECT works by inserting short, intronic sequences into the open reading frame (ORF) of a molecular...molecular tool and adding recombinase recognition sequences (e.g. Lox sites, FRT sites) inside of the introns...The addition of these recombinase recognition sequences enables directional control of the portion of ...

... pegRNA directs the Cas9 nickase to the target sequence where it nicks the non-target strand and generates..., if the edited 3’ flap is excised, the target sequence remains unchanged and available as a substrate...

...by an RNA guide (gRNA) that harbors targeting sequences. Browse our collection of empty gRNA plasmids ...plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used...

...assessment, sterility testing, and viral genome sequencing....Purity assessment Sterility testing Viral genome sequencing (VGS) Find detailed pricing for your chosen plasmid...

...protein (TetR) and operator ( tet O, a 19 nucleotide sequence, TCCCTATCAGTGATAGAGA). In normal conditions, the...called pTet) have been designed by combining tet O sequences with elements like the H1 RNA promoter. And, most...or other species by codon optimization of their sequence and eliminating interactions with endogenous factors...Bujard, H., & Hillen, W. (2000). Exploring the sequence space for tetracycline-dependent transcriptional...

...generates a double-strand break (DSB) at a specific sequence. Two unique gRNAs, binding ~15-25 bp apart, are...

...accessibility in research and therefore makes the sequences of these affinity reagents available to scientists...

...promoter, enhancer, or the beginning of the coding sequence. Figure 1: Overview of CRISPR interference. Browse...

...dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system...

...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...

...expression vectors for insertion of custom gRNA target sequences....

...Each isoform is uniquely barcoded with a 24 bp sequence, to simplify identification during screening. ...

...transformation, including regulatory elements, coding sequences, basic CRISPR parts, and parts for modular geminiviral...

...immunoprecipitation (ChIP) to allow purification of any genomic sequence specified by a particular gRNA. In the enChIP ...

...using a single gRNA that has multiple targeted sequences in close proximity. A non-repetitive genomic locus...

...These plasmids are created by your colleagues, sequence-verified, and widely cited in publications. All...

...assessment, sterility testing, and viral genome sequencing (VGS). In-stock AAV preps are distributed as ...