...transcription initiation when targeted to DNA sequences within core promoter regions. pTAL5-BB contains... place of pTAL1, 2, 3, or 4. For both plasmids sequence positions 1214–2210 of pTAL3 were cloned into ...visualize subcellular positioning of target DNA sequences in living cells (TALE-mediated Genome Visualization...white-screening in E.coli , (iii) CAG promoter and Kozak sequence to drive efficient TALEN expression in mammalian...vectors for use in labeling specific repetitive DNA sequences in human chromosomes Do you have suggestions for...

...please contact us . Full sequencing of the Viral Genome Next-generation sequencing is performed on viral ...genes, and a plasmid encoding adenoviral helper sequences. AAV preparations are purified by iodixanol gradient...genomes isolated from the final AAV preparation. Sequencing results are analyzed to confirm the identity ...

...Does my transcription factor bind to a particular sequence? Do these two proteins interact? What happens ...localization: Contains a 2x array of the binding sequence for RNA-binding protein, MS2. Can be used in combination...localization: Contains a 2x array of the binding sequence for RNA-binding protein MS2 fused to dsRED. Can...Tac (lactose/IPTG inducible) MBP, 6x His, signal sequence for localization to the periplasm David Waugh ...N-terminal 6x His tag and an MBP tag. Contains a signal sequence to localize the protein of interest to the periplasm...of N and C-terminal epitope tags, localization sequences, and cleavage sites that can be used to express...Assembly Accessory Reagents Kit Various Plasmids Sequence chosen by researcher Beta-galactosidase Keith ...

...of target sequence in the 3' UTR David Bartel 12179 pIS1 Renilla Insertion of target sequence in the 3'... Bartel 12177 pIS2 Renilla Insertion of target sequence in the 3' UTR David Bartel 101139 pCMV-IRES-Renilla... PCD FL0X Firefly Insertion of imperfect miRNA sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly... a T7 promoter for normalization. FMDV StopGo sequences flank the MCS for improved normalization. John... mpCHECK2 Firefly/Renilla Insertion of 3' UTR sequences downstream of Renilla luciferase. Firefly luciferase...psi-CHECK3 Firefly/Renilla Insertion of 3' UTR sequences downstream of Renilla luciferase. Firefly luciferase...

... purified, sequenced via the Sanger method, and aligned with the parental plasmid sequence to confirm ...protein size is confirmed. Residual Plasmid Sanger Sequencing Purified recombinant antibody preps contain low...

...specific genomic regions tagged with the recognition sequences of an exogenous DNA-binding protein such as LexA...ver. 2 An empty sgRNA expression vector. sgRNA sequence can be cloned into BbsI site. gRNA scaffold has...30485. doi: 10.1038/srep30485. PubMed . Efficient sequence-specific isolation of DNA fragments and chromatin...telomeres using a combination of enChIP and RNA sequencing. Fujita T, Yuno M, Okuzaki D, Ohki R, Fujii H...

...immunoprecipitation (ChIP) to allow purification of any genomic sequence specified by a particular gRNA. In the enChIP ...For CRISPR-based purification, design your gRNA sequence to direct dCas9 to a specific locus, avoiding ...

...produced by all three methods are identical in sequence. TAL effector repeat arrays produced on this framework... ZiFiT Targeter enables users to search their sequences of interest for potential TALEN or TALE transcriptional...

...of a 19 nucleotide tetracycline operator (tetO) sequence, and is recognized by the tetracycline repressor... the tetR portion of tTA will bind these tetO sequences and the activation domain promotes expression.... tetR. This precludes tTA binding to the tetO sequences and subsequent increase in expression by the activation... EF1α promoter driving tTA; contains insulator sequence; plasmid pEnt L1L3 rtTA-3 is a rtTA version tTA...

...Mismatches between the guide RNA and the target sequence lower the repression level. By controlling the...motifs (squares), and also carrying the tracrRNA sequence (not shown). (B) Relative GFP and RFP concentration...

...other useful protein domains to a specific DNA sequence ( Figure 1A ). Scientists have identified a large...incentives are high. Since ZF targets are confined to sequences composed of triplets with corresponding zinc fingers...Pubmed PMID: 21960622 . Efficient construction of sequence-specific TAL effectors for modulating mammalian...

... for plasmids that contain a zebrafish gene or sequence . The table below highlights plasmids that contain...Zebrafish Genome Project - Access the latest reference sequence assembly for the zebrafish genome. Zebrafish Model...zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been experimentally validated in zebrafish...

...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...

... the following reasons: Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer...Our final step of the QC process, viral genome sequence verification, may not yet have been completed ...

...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...promoter/enhancer or the beginning of the coding sequence. If the plasmid you’re using does not also express...

...double-stranded RNA (dsRNA) that matches the mRNA target sequence. The dsRNA can be delivered as shRNA (short hairpin...remaining single-stranded siRNA then binds the target sequence and RISC cleaves and degrades the mRNA. RISC includes...

... (SARS classic) sequences. Mammalian Targets : Plasmids containing mammalian sequences linked to SARS-...available or coming soon containing SARS-CoV-2 sequences. SARS-CoV Plasmids : Plasmids that are available... Plasmids Many plasmids containing SARS-CoV-2 sequences are now available and more are currently undergoing...

...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...

...abnormally long polyglutamine sequences. These glutamine-rich sequences are prone to misfolding and aggregation...neuroscience research created with high-volume hybridoma sequencing on the NeuroMabSeq platform. Mitchell et al. ...

...orthogonal recombinase (Cre and Flp) recognition sequences within synthetic introns. INTRSECT was first shown...: INTRSECT works by inserting short, intronic sequences into the open reading frame (ORF) of a molecular...molecular tool and adding recombinase recognition sequences (e.g. Lox sites, FRT sites) inside of the introns...The addition of these recombinase recognition sequences enables directional control of the portion of ...