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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 112 results for: cell

Showing: 81 - 112 of 112 results

  1. TALEN Expression Vectors

    Type
    Collection
    ...listed below have the CMV promoter for mammalian cell expression and a T7 promoter for in vitro transcription...

  2. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ... time and are potentially heritable in dividing cells. CRISPR Activate and Interfere technologies also...

  3. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...is highly recommended that you determine if your cell type contains the target sequence before using any...

  4. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...bound to DNA, and the break can be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the...

  5. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...stable imaging of protein interactions in living cells and live mice. Proteins for in vivo imaging have...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics Background In vivo ...

  7. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs from deleting both...

  9. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single...

  10. CRISPR Plasmids - Base Edit

    Type
    Collection
    ... inosine, which is treated like guanosine by the cell, creating an A to G (or T to C) change. Adenine ...

  12. Microbiology Resources

    Type
    Collection
    ...applications like recombinant protein purification and cell-free circuit design in E. coli - Freemont Lab MoClo-YTK...

  13. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...and the resulting heteroduplex is resolved by the cell’s mismatch repair (MMR) system. Alternatively, if...

  14. AAVED

    Type
    Collection
    ...GCaMP6f-P2A-nls-dTomato Detailed protocol: AAV Production in HEK293 Cells Please share materials by uploading to your Dropbox...

  15. Chemogenetics AAV Preps

    Type
    Collection
    ... chemically induce neuronal activity in specific cell types. See our Chemogenetics Plasmids and Chemogenetics...

  16. AAV Packaged on Request

    Type
    Collection
    ... tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments...

  17. Bacterial Expression Systems

    Type
    Collection
    ...tags whose localization you can monitor in live cells (beware of artifacts!), photoactivatable tags for...Purification Sometimes looking at a protein inside cells just isn’t enough to get all the information you... tell you much about its function and/or alter cellular activity in a way that’s interesting from an applied...expression and activity thereby limiting the use of cellular resources required for the production of large...small molecules, light, temperature, or even intracellular metabolite levels. Different plasmids and, often...

  18. Arf GTPase Family

    Type
    Collection
    ...concepts and challenges. Molecular Biology of the Cell , 30 (11), 1249–1271. PMID: 31084567 (Link opens...

  19. TALEN Guide

    Type
    Collection
    .... Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander JD, Cade L, Khayter...

  20. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...R-mAbs plasmids, production of R-mAbs in transfected cells and their validation can be found below. With these...transfecting the plasmids into mammalian tissue culture cells (e.g., HEK293, COS, CHO), from which the R-mAbs ...level expression we recommend using the HEK-2936E cell line engineered by Durocher et al., 2002 (Link opens...used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target Target Species Host Species...Pink1 Human M. musculus (mouse) IgG2b 227033 SynCAM1 cell adhesion molecule (Mus musculus) recombinant mouse...used to produce scFvs from transfected mammalian cells. Addgene ID Plasmid Hybridoma Target Target Species...to produce nanobodies from transfected bacterial cells. Addgene ID Plasmid Hybridoma Target Target Species...

  21. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Cas protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both...Alternatively, gRNA-only plasmids can be used in cells or organisms in which Cas9 has been integrated into...

  22. Optogenetics AAV Preps

    Type
    Collection
    ...Optogenetics AAV Optogenetic tools can be used to control cell activity in living tissue with light. Optogenetics...

  23. Retrograde AAV viral preps

    Type
    Collection
    ... of Cre, and expresses NLS-EGFP in Cre-positive cells. Control Harvey 137164 pAAV-nEF-Con/Fon/Von eYFP...

  24. The Pleiades Promoter Project

    Type
    Collection
    ...therapies for brain disorders by enabling region- and cell-specific gene-delivery in the mouse brain through...

  25. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...University of Arizona Shaner et al. : Journal of Cell Science, Vol. 120, December 2007, pp. 4247-4260 ...Fluorescent Protein Resources: FRET Biosensors Subcellular localization Michael Davidson Collection Blog...

  26. MAPK Plasmids

    Type
    Collection
    ...Mitogen-activated protein kinases (MAPKs), also known as extracellular signal-regulated kinases (ERKs), are serine/...threonine-specific protein kinases. They regulate diverse cellular programs by acting as an integration point for...

  27. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...Protein Resources: Empty Backbones Biosensors Subcellular localization Optogenetics Background Förster ... Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses...

  28. Tags and Other Markers

    Type
    Collection
    ...catalog that target epitope tags and other common cellular proteins. These ready-to-use recombinant monoclonal...

  31. Synthetic Biology - Overview

    Type
    Collection
    ...Sensing and Signaling Browse plasmids related to intercellular signaling and environmental sensing. Strains...
Showing: 81 - 112 of 112 results