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We narrowed to 100 results for: Cel

Showing: 41 - 60 of 100 results

  1. Genetic Code Expansion

    Type
    Collection
    ...applications of genetic code expansion in cell biology . Nat Rev Mol Cell Biol, 13 (3), 168–182. https://doi....expanding the genetic code in bacteria and mammalian cells. Plasmid...which means they work independently of the host cell's natural genetic machinery. The orthogonal aaRS ...while E. coli pairs are orthogonal in eukaryotic cells. Pairs from methanogens like Methanosarcina barkeri... are orthogonal both in bacteria and eukaryotic cells. Many of these sets have been mutated and screened... Once these plasmids have been introduced in the cell, the ncAA provided in the media can be incorporated...match the growth medium, ncAA concentration, and the cell lines used previously. Remember that the orthogonal...

  2. iPSC Neurodegenerative Disease Initiative Plasmid Collection

    Type
    Collection
    ...nucleotide variant lines in an isogenic cellular background. All cell lines and phenotypic datasets that result...CRISPR Guide CRISPR Protein Tagging Plasmids for Stem Cell Research Neurodegeneration Research Collection The...Plasmids The following plasmids can be used to create cell lines with endogenously-tagged gene variants or ...or to differentiate human iPSCs into other cell types, as noted in the purpose field: ID Plasmid Description...neurodegenerative diseases with genomic engineering: A new stem cell initiative from the NIH. Neuron . 2021 Apr 7;109...

  3. p53 Pathway

    Type
    Collection
    ...p53 are in promoting cell cycle arrest, apoptosis, or senescence in damaged cells. The p53 name arose ...transcription factor and tumor suppressor activated by cellular stress. Cancer...transcription factor and tumor suppressor activated by cellular stresses like DNA damage, oxidative stress, and...or E2 Cyclin G CCNG1 CCNG2 Cyclin G1 or G2 CDC25 Cell division cycle 25C Cdk4/6 CDK4 CDK6 Cyclin-dependent...member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor Gadd45 GADD45A GADD45B GADD45G...

  4. NETRF

    Type
    Collection
    ...collaborative NET research community is pivotal in accelerating progress in the diagnosis and treatment of neuroendocrine...and foundations, who share in their vision to accelerate scientific discovery that will help create new... Modeling Neuroendocrine Tumors Using Adult Stem Cell-Derived Organoids Matthew Meyerson Dana-Farber Cancer...Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University...University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley Bernstein Broad Institute...

  5. Cancer Research Plasmids and Resources

    Type
    Collection
    ...important to normal cell signaling and physiology, and, when dysregulated, can enable cancer cell survival. Although...that is essential for regulating the growth of the cell. p53 p53, the most frequently mutated gene in human...and Libraries for Cancer Research Broad Target Accelerator Plasmids: Collection of vectors from the Boehm...mutant alleles described in the initial Target Accelerator publications, focused on somatic variants found...

  6. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...compatible with fluorescence microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting... multiple genomic loci, and compatible with live cell imaging. Compared to techniques like fluorescence... for detecting the chromatin dynamics in living cells. The fluorescent CRISPR system has been used for... loci, as well as chromosome painting in living cells. Visualizing a specific genomic locus requires recruitment...repetitive loci can be efficiently visualized in living cells using a single gRNA that has multiple targeted sequences...

  7. Optogenetics Plasmid Collection

    Type
    Collection
    ...switch tools to activate, inhibit, and manipulate cellular processes....to blue light and allows cations to flow into the cell. Variants of channelrhodopsin have been engineered...improve the expression and membrane targeting in host cells. Other microbial opsins, such as the Halorhodopsins...actively move cations or anions into or out of the cell. When using these opsins as a tool to control neuronal...plasmids that use light to control a protein or cellular activity. For example, search "Cdc42", "transcription...

  8. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...function in zebrafish, plants, and human cells ( Maeder et al., Mol Cell 2008 , Foley & Yeh et al., PLoS ONE... Townsend et al., Nature 2009 , Zou et al., Cell Stem Cell 2009 ). Resources A detailed protocol for practicing...ZFNs that function in zebrafish, plants, and human cells Kits...

  9. Institute for Protein Innovation

    Type
    Collection
    ...unique recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration... with a mission to advance protein science to accelerate research and improve human health. As part of...highly conserved proteoglycans crucial for regulating cell signaling during development. The IPI collection...two heparan sulfate chains and is tethered to the cell surface by a glycosylphosphatidylinositol (GPI) ...antibodies that target epitope tags and other common cellular proteins. ID Recombinant Antibody Description ...

  10. Antibody Production

    Type
    Collection
    ...mammalian expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding the.... This buffer is not compatible for use in live cells and will interfere with conjugation. For applications...antigen is first transiently expressed in mammalian cells. To learn which specific QC experiments were performed...email [email protected] . Immunocytochemistry (ICC) Cells are fixed and labeled with the antibody and a species-specific...Western Blotting (WB) Proteins are extracted from cells, separated by SDS-PAGE, transferred to a membrane...

  11. Antibody Plasmid Collection

    Type
    Collection
    ...expressing these in bacteria, mammalian cells, yeast, plants, or insect cells. Benefits include consistency between... research purposes include animals or hybridoma cells, but are difficult to generate due to expense and...monoclonal antibodies (R-mAbs) expressed in mammalian cells. Monoclonal antibodies are derived from an individual...transiently transfected into mammalian tissue culture cells, the R-mAbs are secreted into the culture media ...

  12. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...promote mitophagy . Molecular Cell , 60 (1), 7–20. https://doi.org/10.1016/j.molcel.2015.08.016. PMID 26365381...abundant forms of posttranslational modification in cells and research into its many roles in protein function...interactions can be discovered by fluorescence-activated cell sorting (FACS). The Mode #2 phosphosite library ... production of recombinant phosphoproteins using cell-free protein synthesis . Nature Communications ,...

  13. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...be used for screening transcription factors for cellular programming or cancer drug resistance....Zhang Lab Plasmids The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled...transcription factors for applications such as cellular programming or cancer drug resistance. Protocols...Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell 2023 Jan 5; 186(1):209-229. PubMed (Link opens in...

  14. Brain Initiative Collection

    Type
    Collection
    ...real-time interrogation and monitoring of brain cells and circuits during behavior. Long desired by researchers...A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human...A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Cre-dependent...genetically encoded dopamine sensor RdLight1 in mammalian cells. Lin Tian 125708-AAV9 pAAV-hSynapsin1-RdLight1 Expresses...neurons Yulong Li 158757-AAV8 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f... Edward Boyden 158757-AAV9 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f...

  15. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single...firefly luciferase to simultaneously measure the # of cells containing reactivated latent provirus and the overall...strength of viral transcriptional response in these cells. Warner Greene 105621 Lenti-luciferase-P2A-Neo Lentiviral...

  16. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...or genetic elements of interest to introduce in cells. A packaging plasmid (or Rep/Cap plasmid) containing...or herpes simplex virus, to replicate in the host cell and complete the lytic cycle. To avoid the use of...provided by helper plasmids and specific packaging cell lines. Helper plasmids generally encode the adenoviral... General Transfection AAV Production in HEK293T Cells AAV Purification by Iodixanol Gradient Ultracentrifugation...

  17. Depositor Collections

    Type
    Collection
    ...Institute for Cell Science Plasmid Collection E11 Bio PRISM Collection New England BioLabs Cell-Imaging Plasmid...Botman-Teusink Yeast FP Collection Protein Expression Cell Migration Consortium Malate Dehydrogenase CUREs ...

  18. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...to activate programmed cell death or dormancy for phage-infected bacterial cells so as to limit the spread...population. Type VI enzymes that function in mammalian cells can be used to attentuate RNA levels. In mammalian...

  19. TALEN Engineering

    Type
    Collection
    ...Tesson et al., Nat Biotechnol. 2011 ) Human somatic cells ( Miller et al., Nat Biotechnol. 2011 ; Reyon and...., Nat Biotechnol 2012 ) Human pluripotent stem cells ( Hockemeyer et al., Nat Biotechnol. 2011 ) TALE...TALE transcriptional activators in human cells ( Maeder et al., Nat Methods 2013 ) More detailed information...

  20. Worm Expression Resources

    Type
    Collection
    ...Worms are particularly well suited for studies in cell biology, development, and aging. Scientists can .... elegans hermaphrodite consists of 959 somatic cells, which makes them attractive for imaging and fluorescence...researchers to express desired constructs in precise cell types and/or developmental timeframes. Here we highlight...
Showing: 41 - 60 of 100 results