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We narrowed to 119 results for: ELL

Showing: 41 - 60 of 119 results

  1. Luciferase Plasmid Collection

    Type
    Collection
    ...not secreted, so cells must be lysed or the substrate must be delivered intracellularly to measure luminescence...Expression of firefly luciferase from a cell-free mammalian cell lysate Marcel Bruchez 108542 pLenti-EF1a-Luciferase-IRES-Blast-WPRE...Insertion of 5' promoter/enhancer regions Joshua Mendell 60323 pGL4.23-GW Firefly Insertion of 5' promoter...expression of luciferase in the cytoplasm of mammalian cells Peter Cockerill 212936 pGL3 Basic Vector Firefly... viral infection efficiency, to study changes in cell physiology, or as a reference level of expression...Expression of Gaussia luciferase; GFP is expressed if cells are infected with virus Christopher Buck 98580 pLX304...membrane-anchored version useful for labeling extracellular vesicles. LumiScarlet : A fusion of LumiLuc ...

  2. AAV Molecular Tools

    Type
    Collection
    ...Jinhyun Kim Tools for Cell Ablation These AAV encode tools that can be used for cell ablation. ID Name Expression...receptor fused to GFP for studying cell ablation. 2 Thomas Jessell , Eiman Azim 45580 pAAV-flex-taCasp3...pro-taCasp3 and TEVp for studying cell ablation. 1, 5 Nirao Shah , Jim Wells Genome Engineering Tools These...Transactivators Affinity Purification Neurophysiology Cell Ablation Genome Engineering Labeling Overexpression...Tools These AAV encode tools that can be used for cell or protein labeling. ID Name Expression System Activity...AAV8-DIO-Reporter viruses infected in postsynaptic cells. 5 Don Arnold Don’t See What You’re Looking For?...

  3. Genetic Code Expansion

    Type
    Collection
    ...expanding the genetic code in bacteria and mammalian cells....codons, and tRNA synthetases are introduced into the cell on plasmids and the new amino acid is introduced...Once these plasmids have been introduced in the cells, the non-canonical amino acid can be incorporated...match the growth medium, ncAA concentration, and the cell lines used previously. Remember that the orthogonal... – GFP for E. coli or mCherry-GFP for mammalian cells. You should also express the protein with and without...tRNA synthetase for use in E.coli and Mammalian Cells. Many of the plasmids also contain one or more copies...coli azi-donorleucine (Anl) Bacterial ATG David Tirrell 62598 pKPY514 phenylalanyl-tRNA synthetase subunit...

  4. p53 Pathway

    Type
    Collection
    ...p53 are in promoting cell cycle arrest, apoptosis, or senescence in damaged cells. The p53 name arose ...top References Unravelling mechanisms of p53-mediated tumour suppression. Bieging KT, Mello SS, Attardi ...transcription factor and tumor suppressor activated by cellular stress. Cancer...transcription factor and tumor suppressor activated by cellular stresses like DNA damage, oxidative stress, and...or E2 Cyclin G CCNG1 CCNG2 Cyclin G1 or G2 CDC25 Cell division cycle 25C Cdk4/6 CDK4 CDK6 Cyclin-dependent...member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor Gadd45 GADD45A GADD45B GADD45G...ML, Ogryzko V, Gardner K, Giordano A, Levine AS, Kelly K. Nature. 1997 Jun 27;89(7):1175-84. PubMed PMID...

  5. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... Anti-Pan-Neurofascin (extracellular) [A12/18R] Pan-Neurofascin (extracellular) Rat Mouse IgG2a 177439...Anti-Kv1.4 K+ channel (extracellular) [L71/5R] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 177489...Mouse IgG2a 177564 Anti-ADAM22 (extracellular) [N57/2R] ADAM22 (extracellular) Mouse Mouse IgG2a 177565 Anti-GluN2B...Anti-Kv1.4 K+ channel (extracellular) [L71/22] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 190292...-Neurofascin (extracellular) scFv [A12/18] A12/18 scFv 2t Pan-Neurofascin (extracellular) Rat Mouse 190486...Kv1.4 K+ channel (extracellular) scFv [L71/5] L71/5 scFv Kv1.4 K+ channel (extracellular) Rat Mouse 190518... Mouse 190537 LRP4 (extracellular) scFv [N207/27] N207/27 scFv LRP4 (extracellular) Mouse Mouse 190538...

  6. Control AAV Preps

    Type
    Collection
    ... pAAV-mDlx-GFP-Fishell-1 Dlx GFP Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB Gordon Fishell 99130 pAAV-mDlx-NLS-mRuby2... pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Cre dependent 1, 2, 8, 9, rg* Gordon Fishell 83894 pAAV-hDlx-Flex-dTomato-Fishell...pAAV-hDlx-Flex-dTomato-Fishell_7 Dlx dTomato Cre dependent 1, 2, 5, 9, rg* Gordon Fishell 100043 pAAV.synP.DIO.EGFP.WPRE.hGH...pAAV-CBh-mKate2-IRES-MCS (WT-WT) CBh mKate2 Constitutive 2 Marcella Patrick 114469 pAAV-CaMKIIa-mCherry CaMKIIa mCherry...

  7. iPSC Neurodegenerative Disease Initiative Plasmid Collection

    Type
    Collection
    ...nucleotide variant lines in an isogenic cellular background. All cell lines and phenotypic datasets that result...CRISPR Guide CRISPR Protein Tagging Plasmids for Stem Cell Research Neurodegeneration Research Collection The...Plasmids The following plasmids can be used to create cell lines with endogenously-tagged gene variants or ...or to differentiate human iPSCs into other cell types, as noted in the purpose field: ID Plasmid Description...neurodegenerative diseases with genomic engineering: A new stem cell initiative from the NIH. Neuron . 2021 Apr 7;109...

  8. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...-repetitive genomic loci, as well as chromosome painting in living cells. Visualizing a specific genomic...compatible with fluorescence microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting... multiple genomic loci, and compatible with live cell imaging. Compared to techniques like fluorescence... for detecting the chromatin dynamics in living cells. The fluorescent CRISPR system has been used for...repetitive loci can be efficiently visualized in living cells using a single gRNA that has multiple targeted sequences...

  9. Institute for Protein Innovation

    Type
    Collection
    ... of cell surface and secreted proteins (glypicans and integrins) and common epitope tags, as well as antibody...unique recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration...mission, IPI is providing access to high-quality, well-characterized antibodies in an open science model...highly conserved proteoglycans crucial for regulating cell signaling during development. The IPI collection...antibodies that target epitope tags and other common cellular proteins. ID Recombinant Antibody Description ...

  10. Optogenetics Plasmid Collection

    Type
    Collection
    ...kinetic properties as well as improve the expression and membrane targeting in host cells. Other microbial ...switch tools to activate, inhibit, and manipulate cellular processes. Plasmid...to blue light and allows cations to flow into the cell. Variants of channelrhodopsin have been engineered...actively move cations or anions into or out of the cell. When using these opsins as a tool to control neuronal...plasmids that use light to control a protein or cellular activity. For example, search "Cdc42", "transcription...

  11. CRISPR References and Information

    Type
    Collection
    ...interest and correspond to edited and non-edited cell populations. For more information, see the Hsiau...nucleases (e.g. base editing). Developed by the Luca Pinello Lab (Link opens in a new window) . For more details...), clone (primer design), and evaluate gRNAs, as well as predict off-targets, for CRISPR in 180+ genomes...window) A sgRNA design tool for genome editing, as well as gene regulation (repression and activation). ...identify novel gRNA target sites in a single gene, as well as a target site common to a set of similar sequences...138 KB Musunuru CRISPRs in human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook...Bauer Protocol for Genomic Deletions in Mammalian Cell Lines pSpCas9(BB) (pX330) PDF, 330 KB Parrott NEBuilder...

  12. Retrovirus Plasmids

    Type
    Collection
    ...reverse transcriptase and integrase. Once in a target cell, the virus uses the reverse transcriptase in tow...γ-retroviruses are that they can only transduce dividing cells (this is because they are only able to enter the...of Large T antigen for creation of immortalized cells (see Weinberg Lab page for resistance variants) ...Expression of hTERT for creation of immortalized cells; additional plasmids include Puro resistance and...inducible expression of GFP T2A Cre fusion in mammalian cells Foijer 47916 pRXTN CMV/MSV pRXTN is a modified version...MoMSV Stable expression of luciferase in mammalian cells Wong Return to Top Do you have suggestions for other...

  13. Antibody Plasmid Collection

    Type
    Collection
    ...expressing these in bacteria, mammalian cells, yeast, plants, or insect cells. Benefits include consistency between...application-specific validation and quality control by Addgene as well as by our trusted partner labs. Learn More Cloning... research purposes include animals or hybridoma cells, but are difficult to generate due to expense and...monoclonal antibodies (R-mAbs) expressed in mammalian cells. Monoclonal antibodies are derived from an individual...transiently transfected into mammalian tissue culture cells, the R-mAbs are secreted into the culture media ...

  14. Worm Expression Resources

    Type
    Collection
    ...maintenance. Worms are particularly well suited for studies in cell biology, development, and aging. Scientists.... elegans hermaphrodite consists of 959 somatic cells, which makes them attractive for imaging and fluorescence...microscopy experiments. The C. elegans genome is well studied and hundreds of reporters have been developed...researchers to express desired constructs in precise cell types and/or developmental timeframes. Here we highlight...detection of genome-editing events. Developed in Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient...

  15. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism...PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair ... time and are potentially heritable in dividing cells. ID Plasmid Gene/Insert Selectable Marker PI Publication...DNA. Type VI enzymes that function in mammalian cells can be used to attentuate RNA levels. In mammalian...genomic loci using fluorescent microscopy in living cells. Design your gRNA sequence to direct the dCas9-FP...Cas protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both...

  16. Lentivirus Plasmids

    Type
    Collection
    ...Lentivirus can transduce both dividing and non-dividing cells without a significant immune response. These viruses... in the viral particle) is delivered to a target cell upon infection. When lentivirus is used for research...the researcher wants delivered to specific target cells. This genome is encoded by plasmids called "transfer...encoded by the transfer plasmid is toxic to mammalian cells, it can be difficult to efficiently produce viral...of viral transduction. Also see plasmid 46948 . Elledge 44011 pInducer10-mir-RUP-PheS 3rd Lentiviral shRNA...transduction. See article for more similar plasmids. Elledge 21373 pHIV-EGFP 3rd EF-1alpha driven expression...

  17. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...function in zebrafish, plants, and human cells ( Maeder et al., Mol Cell 2008 , Foley & Yeh et al., PLoS ONE... Townsend et al., Nature 2009 , Zou et al., Cell Stem Cell 2009 ). Resources A detailed protocol for practicing...ZFNs that function in zebrafish, plants, and human cells Kits...

  18. Antibody Production

    Type
    Collection
    ...mammalian expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding the.... This buffer is not compatible for use in live cells and will interfere with conjugation. For applications...antigen is first transiently expressed in mammalian cells. To learn which specific QC experiments were performed...email [email protected] . Immunocytochemistry (ICC) Cells are fixed and labeled with the antibody and a species-specific...Western Blotting (WB) Proteins are extracted from cells, separated by SDS-PAGE, transferred to a membrane...

  19. Chemogenetics AAV Preps

    Type
    Collection
    ...pAAV-hDlx-GiDREADD-dTomato-Fishell-5 hM4D(Gi) - Inhibition NLS-dTomato none 1, 9, rg* Gordon Fishell 83897 pAAV-hDlx-GqDREADD-dTomato-Fishell...pAAV-hDlx-GqDREADD-dTomato-Fishell-4 hM3D(Gq) - Activation NLS-dTomato none 1, 9, rg* Gordon Fishell 50472 pAAV-GFAP-HA-rM3D... chemically induce neuronal activity in specific cell types. See our Chemogenetics Plasmids and Chemogenetics...

  20. Microbiology Resources

    Type
    Collection
    ... Ralstonia solanacearum Rickettsia sp. Salmonella sp. Shigella sp. Staphylococcus sp. Streptococcus sp...Species Anabaena sp. Bacillus sp. Burkholderia sp. Bordetella pertussis Campylobacter sp. Chlamydia sp. Clostridium...reading frames for vaccinia virus - Bennink and Yewdell Labs Yarrowia lipolytica Golden Gate tool kit :...applications like recombinant protein purification and cell-free circuit design in E. coli - Freemont Lab MoClo-YTK...
Showing: 41 - 60 of 119 results