We narrowed to 101 results for: Eras

...plasmids to test whether or not you've efficiently generated infectious virus, plasmids expressing both the...process for packaging these transfer vectors and generating new viral vectors. Download the Lentivirus Transfer...coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP...72268 xpress gene of interest from the PGK (phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express... from pAdenoX from Clontech and can be used to generate adenovirus expressing a variety of reporters as...

...function, modeling diseases, and advancing gene therapy development. In order to produce recombinant AAV...guide for more information about AAV components, generations, viral production, and viral safety. AAV Vector...terminal repeats (ITRs). This table contains a general list of all the transfer plasmids in Addgene's ...specific packaging cell lines. Helper plasmids generally encode the adenoviral E4, E2a, and VA genes. This...Addgene's Biosafety Resources Addgene Protocols General Transfection AAV Production in HEK293T Cells AAV...

...James M. Wilson AV-1-PV0105 105532-AAV1 pAAV.CMV.ffLuciferase.SV40 Control James M. Wilson AV-1-PV1917 105541...James M. Wilson AV-8-PV0105 105532-AAV8 pAAV.CMV.ffLuciferase.SV40 Control James M. Wilson AV-8-PV0146 105535...Karl Deisseroth AV-5-PV0105 105532-AAV5 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-5-PV1090 105537-AAV5... M. Wilson AV-8-PV1302 105538-AAV8 pENN.AAV.TBG.PI.ffLuciferase.RBG James M. Wilson AV-8-PV3637 65015-...Karl Deisseroth AV-9-PV0105 105532-AAV9 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-9-PV0109 105533-AAV9...Karl Deisseroth AV-2-PV0105 105532-AAV2 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-1-PV3365 105554-AAV1...

...Experimental Considerations Recombinase Recognition Sites Each enzyme functions analogously but interacts only...molecules (in trans), a translocation event is generated between the target sites. Created with BioRender.com...such as Drosophila and mouse, particularly in proliferating cells. Expression may cause repetitive recombination...Use this AAV CRISPR knockout library expressing luciferase and Cre to interrogate tumorigenesis in vivo ...

...maintains a Standard Operating Procedure (Link opens in a new window) with general principles for working... in well-defined cellular sub-populations has generally been limited to single-component cellular definitions... cotransmission evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons. Elife... The central amygdala controls learning in the lateral amygdala. Nat Neurosci. 20(12):1680-1685. PubMed...

...expectations communicated by email will supersede the general guarantees above. We will have our expert viral...Production of a Packaged on Request material is generally ready for shipment within 8 weeks after the approval...queue is too high and we do not expect to meet the general turnaround time, Addgene will notify you by email...expectations communicated by email will supersede the general estimated turnaround time above. After starting...

...proteins then express and process the CRISPR loci to generate CRISPR RNAs (crRNAs). Through sequence homology...or repression. Combined with the power of next-generation sequencing, CRISPR is a robust system for genome-wide...RNA. Inosine is translated as guanosine, so the overall effect is an A->G change. Since this method targets...technologies. Biotechnology companies are exploring therapeutic applications of CRISPR to treat genetic disease...ethics associated with CRISPR in agricultural and therapeutic applications. Despite the ethical controversies...

...animals or hybridoma cells, but are difficult to generate due to expense and time. An alternative approach...fragment libraries from chickens Methods for the generation of chicken monoclonal antibody fragments by phage... 2015 Oct;167(2):95-102. Joanna Bereta Sybody Generation Toolbox for making synthetic nanobodies (sybodies...membrane proteins. Elife. 2018 May 24;7. and Generation of synthetic nanobodies against delicate proteins...

...individually. CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm...Jorgensen Lab. This kit contains 29 plasmids used to generate transgenic nematodes, including C. elegans and...Christian Frøkjær-Jensen's Wormbuilder website. General Tools Fire Lab C. elegans Vector Kit - Andrew Fire...plasmids - Paul Sternberg Lab. Plasmids for the temperature-robust GAL4-UAS system in C. elegans . Synthetic...

...vision to accelerate scientific discovery that will help create new and more effective therapies for these...collaborative NET research community is pivotal in accelerating progress in the diagnosis and treatment of neuroendocrine...

...plasmids to express SARS-CoV-2 viral proteins in several expression systems....window) Researchers developing novel diagnostics, therapeutics, and vaccines against COVID-19 all rely on DNA...researchers to express SARS-CoV-2 viral proteins in several expression systems. Addgene partnered with Ginkgo...

...tool to control neuronal activity, scientists generally attach a fluorescent protein to the opsin in order... of proteins can also be controlled by light, generally by utilizing a light sensitive protein from cryptochromes...used as an optical switch to control protein interactions, dimerization, localization or activity of many...

...channelrhodopsin. When the luciferase enzyme oxidizes its substrate (luciferin), it generates light that activates...luminescent opsins: fusion proteins of a light-emitting luciferase and a light-sensing optogenetic element such ...

...quality of research output, RESOLUTE expects to accelerate the pace of research in the field of SLCs to ... deorphanizing engine. Genetic and proteomic interactions represent the framework that provides biological.... Protein production is used for assays and to generate antibody/ high-affinity ligands. Data is embedded...

...siRNAs): mechanism, therapeutic targets, and delivery strategies for cancer therapy . International Journal... genes with potentially harmful mutations in therapeutic settings. As a tool in mammalian cell systems...

...Cas enzymes generate double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand... promoting DSB formation. Delete Cascade-Cas3 generates large genomic deletions. Insert CRISPR transposases...

...produce recombinant adenoviral vectors, researchers generally need to use two plasmids: A shuttle plasmid (or...more information about adenoviral components, generations, viral production, and viral safety. Adenoviral...Videos Addgene's Biosafety Resources Protocols General Transfection Bert Vogelstein’s lab practical guide...

...Additional Resources Protein-barcode Reconstruction by Iterative Staining with Molecular annotations (PRISM) is...Stochastic infection of pooled AAVs delivered in vivo generates unique combinations of protein bits. B) Multiplex..., H.G.J., Leible, D., Leung, K.S., Magno, C.A., Meeran, A., Michalska, J.M., Rieger, F., Wang, C., Wu,...

...sorting process. This combination allows IPI to generate antibodies to highly conserved proteins and other... with a mission to advance protein science to accelerate research and improve human health. As part of... specific αβ subunit pairs — to block ligand interactions, disrupt integrin function, and/or detect integrin...

...England Biolabs has deposited plasmids at Addgene to generate SNAP, CLIP, ACP or MCP tagged plasmids for visualization... is based on the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein, and is suitable...and the ACP and SFP Synthases. Users may still generate their own fluorescent or non-fluorescent CoA substrates...