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We narrowed to 112 results for: Eras

Showing: 41 - 60 of 112 results

  1. Antibody Production

    Type
    Collection
    ...produced in-house by Addgene scientists. They are generated using standard methods individually optimized ...optimized for each specific antibody in order to generate high quality preparations. Following production, all...using ImageJ software and a standard curve is generated. The final antibody concentration is calculated...labeled with the antibody and a species-specific horseradish peroxidase (HRP)-conjugated secondary antibody...passed stability testing, it may ship at room temperature. However, Addgene determines ideal shipping conditions...

  2. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...plasmids to test whether or not you've efficiently generated infectious virus, plasmids expressing both the...process for packaging these transfer vectors and generating infectious virus. Protocol: Lentivirus Transfer...coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP...72268 xpress gene of interest from the PGK (phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express... from pAdenoX from Clontech and can be used to generate adenovirus expressing a variety of reporters as...

  3. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...James M. Wilson AV-1-PV0105 105532-AAV1 pAAV.CMV.ffLuciferase.SV40 Control James M. Wilson AV-1-PV1917 105541...James M. Wilson AV-8-PV0105 105532-AAV8 pAAV.CMV.ffLuciferase.SV40 Control James M. Wilson AV-8-PV0146 105535...Karl Deisseroth AV-5-PV0105 105532-AAV5 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-5-PV1090 105537-AAV5... M. Wilson AV-8-PV1302 105538-AAV8 pENN.AAV.TBG.PI.ffLuciferase.RBG James M. Wilson AV-8-PV3637 65015-...Karl Deisseroth AV-9-PV0105 105532-AAV9 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-9-PV0109 105533-AAV9...Karl Deisseroth AV-2-PV0105 105532-AAV2 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-1-PV3365 105554-AAV1...

  4. Deisseroth INTRSECT Collection

    Type
    Collection
    ...maintains a Standard Operating Procedure (Link opens in a new window) with general principles for working... in well-defined cellular sub-populations has generally been limited to single-component cellular definitions... cotransmission evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons. Elife... The central amygdala controls learning in the lateral amygdala. Nat Neurosci. 20(12):1680-1685. PubMed...

  5. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...proteins then express and process the CRISPR loci to generate CRISPR RNAs (crRNAs). Through sequence homology...or repression. Combined with the power of next-generation sequencing, CRISPR is a robust system for genome-wide...RNA. Inosine is translated as guanosine, so the overall effect is an A->G change. Since this method targets...technologies. Biotechnology companies are exploring therapeutic applications of CRISPR to treat genetic disease...ethics associated with CRISPR in agricultural and therapeutic applications. Despite the ethical controversies...

  6. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...expectations communicated by email will supersede the general guarantees above. We will have our expert viral...Production of a Packaged on Request material is generally ready for shipment within 8 weeks after the approval...queue is too high and we do not expect to meet the general turnaround time, Addgene will notify you by email...expectations communicated by email will supersede the general estimated turnaround time above. After starting...

  7. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...amplify the pooled library and the use of Next-Generation Sequencing (NGS) to confirm library representation...Filters Name ID Library Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson DNA Repair...Libraries 213927, 213928 Knockout Cow Tan 2nd 4 on average 94,000 Bradley Human CRISPR Poison Exon Knockout...aeruginosa X. Liu NA 1 5,981 (Set 1) 5,971 (Set 2) CHyMErA Paralog & Dual-targeting hgRNA pooled library 155199...Knockout Human Moffat Blencowe 3rd variable 92,746 CHyMErA Exon-deletion hgRNA pooled library 155200 Knockout...

  8. Adenovirus Plasmids

    Type
    Collection
    ... viral capsid. Adenoviruses can be produced by several methods, the most common of which involves homologous...transfected into mammalian packaging cell lines to generate adenovirus particles. Read our Adenoviral Guide...terms Adenoviral Strains Bacterial strains for generating adenovirus. ID Strain Description PI 16398 BJ5183...for seamless assembly of multiple mutations, alterations, or transgene insertions into single full-length...

  9. Nuclear Receptor Signaling Atlas (NURSA) Plasmid Guide: Nuclear Receptors

    Type
    Collection
    ...receptors are: 1) a ligand binding domain, which interacts with hormones or other molecules, and 2) a DNA....5, NR1C1, PPAR, PPARalpha, hPPAR peroxisome proliferator-activated receptor alpha PPARD Plasmids PPARD..., NR1C2, NUC1, NUCI, NUCII, PPARB peroxisome proliferator-activated receptor delta PPARG Plasmids PPARG... NR1C3, PPARG1, PPARG2, PPARgamma peroxisome proliferator-activated receptor gamma RARA Plasmids RARA,...

  10. Antibody Plasmid Collection

    Type
    Collection
    ...animals or hybridoma cells, but are difficult to generate due to expense and time. An alternative approach...fragment libraries from chickens Methods for the generation of chicken monoclonal antibody fragments by phage... 2015 Oct;167(2):95-102. Joanna Bereta Sybody Generation Toolbox for making synthetic nanobodies (sybodies...membrane proteins. Elife. 2018 May 24;7. and Generation of synthetic nanobodies against delicate proteins...

  11. Worm Expression Resources

    Type
    Collection
    ...individually. CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm...Jorgensen Lab. This kit contains 29 plasmids used to generate transgenic nematodes, including C. elegans and...Christian Frøkjær-Jensen's Wormbuilder website. General Tools Fire Lab C. elegans Vector Kit - Andrew Fire...plasmids - Paul Sternberg Lab. Plasmids for the temperature-robust GAL4-UAS system in C. elegans . Synthetic...

  12. NETRF

    Type
    Collection
    ...vision to accelerate scientific discovery that will help create new and more effective therapies for these...collaborative NET research community is pivotal in accelerating progress in the diagnosis and treatment of neuroendocrine...

  13. Fluorescent Protein Guide

    Type
    Collection
    ... Resources You May Also Like... Luminescence: Luciferase Plasmids Blog: Which FP Should I Use? Blog: How...pairs and standards to study protein-protein interactions or conformational changes within a protein. ...study individual plasmids or protein-protein interactions in organs and whole mammals. Regulate Biological...

  14. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...study individual plasmids or protein-protein interactions in deep-tissue organs and whole mammals. Genetically...probes are suitable for stable imaging of protein interactions in living cells and live mice. Proteins for ...complementation probe used for detecting protein-protein interactions in vitro and in vivo . Learn more about in vivo...

  15. Chemogenetics Plasmids

    Type
    Collection
    ...actuator. When the luciferase enzyme oxidizes its substrate (luciferin), it generates light that activates...luminescent opsins: fusion proteins of a light-emitting luciferase and a light-sensing optogenetic element, making...

  16. Optogenetics Plasmid Collection

    Type
    Collection
    ...tool to control neuronal activity, scientists generally attach a fluorescent protein to the opsin in order... of proteins can also be controlled by light, generally by utilizing a light sensitive protein from cryptochromes...used as an optical switch to control protein interactions, dimerization, localization or activity of many...

  17. Resolute Plasmid Collection

    Type
    Collection
    ...quality of research output, RESOLUTE expects to accelerate the pace of research in the field of SLCs to ... deorphanizing engine. Genetic and proteomic interactions represent the framework that provides biological.... Protein production is used for assays and to generate antibody/ high-affinity ligands. Data is embedded...

  18. Mammalian RNAi Tools

    Type
    Collection
    ...siRNAs): mechanism, therapeutic targets, and delivery strategies for cancer therapy . International Journal... genes with potentially harmful mutations in therapeutic settings. As a tool in mammalian cell systems...

  19. CRISPR Plasmids and Resources

    Type
    Collection
    ...Cas enzymes generate double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand...promoting DSB formation. Delete NEW Cascade-Cas3 generates large genomic deletions. Insert NEW CRISPR transposases...

  20. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...England Biolabs has deposited plasmids at Addgene to generate SNAP, CLIP, ACP or MCP tagged plasmids for visualization... is based on the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein, and is suitable...and the ACP and SFP Synthases. Users may still generate their own fluorescent or non-fluorescent CoA substrates...
Showing: 41 - 60 of 112 results