...Class I PIN1 Peptidylprolyl cis/trans isomerase, NIMA-interacting 1 PLXNB1 Plexin B1 PPP1CA Protein phosphatase...relationship. The content and map for this page were generated with the help of Dominic Esposito and the Frederick... related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral...protein 2 ICMT Isoprenylcysteine carboxyl methyltransferase INSR Insulin receptor INSRR Insulin receptor-related... . Targeting RAS signalling pathways in cancer therapy. Downward J. Nat Rev Cancer. 2003 Jan;3(1):11-22...

...transduction of the trabecular meshwork following intracameral injection in mice, rats, and dogs. This serotype...serotype displays highly efficient transduction and lateral spread in the retina following subretinal injection...E531D) serotype has increased transduction and lateral spread in the retina following subretinal injection...Transduction optimization of AAV vectors for human gene therapy of glaucoma and their reversed cell entry characteristics...Display Exceptional Characteristics for Retinal Gene Therapy. Mol Ther . Jun 3;28(6):1464-1478. PMID: 32304666...

...and throughput over traditional tagging methods. Several different tagging techniques, as well as the plasmids...plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt Unc-32::GFP targeting...Auxin-inducible degron (AID) tagging with CRISPR/Cas to generate conditional alleles of essential nuclear and cytoplasmic...technique for use in Drosophila cells that uses PCR to generate both an expression cassette for the Cas9-programming...cells 3.3 MB Seydoux C. elegans Tagging System Geraldine Seydoux's lab has developed a systematic and scalable...elegans . This system uses a 10-day protocol, generates “clean” homozygous mutants with no co-integrated...

...18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also expresses luciferase Lowe 9044 pMIG...gamma-retrovirus) is an RNA virus comprised of its genome and several structural and enzymatic proteins, including reverse...virus uses the reverse transcriptase in tow to generate a DNA provirus. This provirus then gets integrated...Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in mammalian cells Wong Return to Top Do you ...

...produced in-house by Addgene scientists. They are generated using standard methods individually optimized ...optimized for each specific antibody in order to generate high quality preparations. Following production, all...using ImageJ software and a standard curve is generated. The final antibody concentration is calculated...labeled with the antibody and a species-specific horseradish peroxidase (HRP)-conjugated secondary antibody...passed stability testing, it may ship at room temperature. However, Addgene determines ideal shipping conditions...

...plasmids to test whether or not you've efficiently generated infectious virus, plasmids expressing both the...process for packaging these transfer vectors and generating infectious virus. Protocol: Lentivirus Transfer...coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP...72268 xpress gene of interest from the PGK (phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express... from pAdenoX from Clontech and can be used to generate adenovirus expressing a variety of reporters as...

...James M. Wilson AV-1-PV0105 105532-AAV1 pAAV.CMV.ffLuciferase.SV40 Control James M. Wilson AV-1-PV1917 105541...James M. Wilson AV-8-PV0105 105532-AAV8 pAAV.CMV.ffLuciferase.SV40 Control James M. Wilson AV-8-PV0146 105535...Karl Deisseroth AV-5-PV0105 105532-AAV5 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-5-PV1090 105537-AAV5... M. Wilson AV-8-PV1302 105538-AAV8 pENN.AAV.TBG.PI.ffLuciferase.RBG James M. Wilson AV-8-PV3637 65015-...Karl Deisseroth AV-9-PV0105 105532-AAV9 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-9-PV0109 105533-AAV9...Karl Deisseroth AV-2-PV0105 105532-AAV2 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-1-PV3365 105554-AAV1...

...maintains a Standard Operating Procedure (Link opens in a new window) with general principles for working... in well-defined cellular sub-populations has generally been limited to single-component cellular definitions... cotransmission evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons. Elife... The central amygdala controls learning in the lateral amygdala. Nat Neurosci. 20(12):1680-1685. PubMed...

...proteins then express and process the CRISPR loci to generate CRISPR RNAs (crRNAs). Through sequence homology...or repression. Combined with the power of next-generation sequencing, CRISPR is a robust system for genome-wide...RNA. Inosine is translated as guanosine, so the overall effect is an A->G change. Since this method targets...technologies. Biotechnology companies are exploring therapeutic applications of CRISPR to treat genetic disease...ethics associated with CRISPR in agricultural and therapeutic applications. Despite the ethical controversies...

...expectations communicated by email will supersede the general guarantees above. We will have our expert viral...Production of a Packaged on Request material is generally ready for shipment within 8 weeks after the approval...queue is too high and we do not expect to meet the general turnaround time, Addgene will notify you by email...expectations communicated by email will supersede the general estimated turnaround time above. After starting...

...amplify the pooled library and the use of Next-Generation Sequencing (NGS) to confirm library representation...Filters Name ID Library Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson DNA Repair...Libraries 213927, 213928 Knockout Cow Tan 2nd 4 on average 94,000 Bradley Human CRISPR Poison Exon Knockout...Blasticidin) Activation Human Zhang 3rd 3 70,290 CHyMErA Paralog & Dual-targeting hgRNA pooled library 155199...Knockout Human Moffat Blencowe 3rd variable 92,746 CHyMErA Exon-deletion hgRNA pooled library 155200 Knockout...

... viral capsid. Adenoviruses can be produced by several methods, the most common of which involves homologous...transfected into mammalian packaging cell lines to generate adenovirus particles. Read our Adenoviral Guide...terms Adenoviral Strains Bacterial strains for generating adenovirus. ID Strain Description PI 16398 BJ5183...for seamless assembly of multiple mutations, alterations, or transgene insertions into single full-length...

...receptors are: 1) a ligand binding domain, which interacts with hormones or other molecules, and 2) a DNA....5, NR1C1, PPAR, PPARalpha, hPPAR peroxisome proliferator-activated receptor alpha PPARD Plasmids PPARD..., NR1C2, NUC1, NUCI, NUCII, PPARB peroxisome proliferator-activated receptor delta PPARG Plasmids PPARG... NR1C3, PPARG1, PPARG2, PPARgamma peroxisome proliferator-activated receptor gamma RARA Plasmids RARA,...

...animals or hybridoma cells, but are difficult to generate due to expense and time. An alternative approach...fragment libraries from chickens Methods for the generation of chicken monoclonal antibody fragments by phage... 2015 Oct;167(2):95-102. Joanna Bereta Sybody Generation Toolbox for making synthetic nanobodies (sybodies...membrane proteins. Elife. 2018 May 24;7. and Generation of synthetic nanobodies against delicate proteins...

...individually. CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm...Jorgensen Lab. This kit contains 29 plasmids used to generate transgenic nematodes, including C. elegans and...Christian Frøkjær-Jensen's Wormbuilder website. General Tools Fire Lab C. elegans Vector Kit - Andrew Fire...plasmids - Paul Sternberg Lab. Plasmids for the temperature-robust GAL4-UAS system in C. elegans . Synthetic...

...disease in humans. For these reasons, AAVs are generally contained at lower biosafety levels and elicit...plasmids that are useful to produce AAV. In order to generate infectious AAV particles, a helper plasmid (encoding...sequence Lu AAV Plasmids This table contains a general list of plasmids that are useful in the production...contained in the virion) and/or can be used to generate infectious AAV particles. Additional helper plasmids...

...vision to accelerate scientific discovery that will help create new and more effective therapies for these...collaborative NET research community is pivotal in accelerating progress in the diagnosis and treatment of neuroendocrine...

... Resources You May Also Like... Luminescence: Luciferase Plasmids Blog: Which FP Should I Use? Blog: How...pairs and standards to study protein-protein interactions or conformational changes within a protein. ...study individual plasmids or protein-protein interactions in organs and whole mammals. Regulate Biological...

...study individual plasmids or protein-protein interactions in deep-tissue organs and whole mammals. Genetically...probes are suitable for stable imaging of protein interactions in living cells and live mice. Proteins for ...complementation probe used for detecting protein-protein interactions in vitro and in vivo . Learn more about in vivo...

...actuator. When the luciferase enzyme oxidizes its substrate (luciferin), it generates light that activates...luminescent opsins: fusion proteins of a light-emitting luciferase and a light-sensing optogenetic element, making...