...removing the amplification and extraction steps required when one receives plasmids in bacterial stabs....yield slightly different DNA concentrations. Can I directly transfect cloning grade DNA into cells? Cloning...DNA is designed for use in cloning applications. Direct transfection into cells is not recommended. Can...independently including the check out and will require separate shipping charges. Where can I find cloning...

...for direct injection. These AAV are made from plasmid 50465 (deposited by Bryan Roth ) and direct EGFP...Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made from plasmid 37825...37825 (deposited by Edward Boyden ) and direct GFP expression from the CAG promoter. For information about...

...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...present, the cell may repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental...of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions...plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids....

...following in mind: CRISPR screening experiments require electroporation to amplify the pooled library and...library representation. CRISPR screening experiments require large quantities of cells and therefore, may not...limited (e.g. primary cells). Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 ...of gRNAs - A given CRISPR library may target the entire genome or a specific class of genes (subpools)....Library 112200 Knockout Human Lin 3rd 4-5 8,382 Human Paired-guide RNA (pgRNA) Library for Long Non-coding RNAs...

...(PBS) and the desired edits on an RT template. During prime editing, the pegRNA directs the Cas9 nickase...that binds to a specific genomic DNA locus and directs the nickase to the target site. The longer pegRNA...3’ flap binds to the PBS of the pegRNA and the desired edit is incorporated into the DNA by reverse transcription...

...interest and effort directed toward expanding this capacity. Traditionally, AAV requires the presence of ... products that mediate AAV replication. This requirement has been circumvented with “helper-virus free...(which may not be available at Addgene) may be required to produce infectious AAV particles. You can search...

...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...cell may instead repair the break using homology-directed repair (HDR). HDR is much less error-prone than...plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids....

...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...present, the cell may repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental...plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids....

...coinjected to generate mutations via imperfectly repaired DSBs. Coinjection of phsp70-Cas9 and plasmids ...Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence...vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed...

...charges may be required before your order will be delivered. The courier will contact you directly regarding...additional customs documentation that might be required by your country for the import of biological research...

...material. The mitochondria produces the energy cells required for chemical reactions. Labeling these structures...Chromatin H2B mRFP Bryan Welm, Zena Werb 21045 pH2B_mCherry_IRES_puro2 Chromatin H2B mCherry Daniel Gerlich 25999... alpha-tubulin mCherry* Michael Davidson 12298 pIRESneo-EGFP-alpha Tubulin Microtubules alpha-tubulin ... the proteins that enter the secretory pathway require chaperones to help with folding or post-translational...

...library expresses EGFP for easy visualization via direct fluorescence. Morris 115644 Morris CellTag Barcode...library expresses EGFP for easy visualization via direct fluorescence. Morris 115645 Morris CellTag Barcode...library expresses EGFP for easy visualization via direct fluorescence. Morris Control Lentivirus Browse ...

...with two homology arms on each side flanking your desired insertion or mutation. Each arms is around 800bp... in the HR template. Since the PAM sequence is required for successful targeting and cleavage, changing...with two homology arms on each side flanking your desired insertion or mutation. Each arm is around 800bp...

...individual clones, or the pooled library containing the entire pooled collection, can be purchased at Addgene....Protocols & References A transcription factor atlas of directed differentiation. Joung J, Ma S, Tay T, Geiger-...

...nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (...template to introduce specific edits via homology-directed repair (HDR). Want more information on the wide...

...provides a stark contrast to Cas9 and Cpf1, which require that each DNA target have high sequence identity...to limit the spread of infection throughout the entire population. Type VI enzymes that function in mammalian...

...living cells. Visualizing a specific genomic locus requires recruitment of many copies of labeled proteins...gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target sites...

...purification methods are more straightforward and enable direct identification of molecules associated with a genomic...CRISPR-based purification, design your gRNA sequence to direct dCas9 to a specific locus, avoiding known transcription...

...TnsC, and TniQ). Type I CASTs can result in bidirectional insertions and may not be suitable for applications... I, type V-K CASTs appear to exhibit almost unidirectional insertions, but are more prone to off-target...

...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...present, the cell may repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental...