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Showing: 41 - 60 of 76 results

  1. Chemogenetics Plasmids

    Type
    Collection
    ... function and examine the relationship between neuronal signaling and behavior. Depending on the signaling...the opsin to either excite or inhibit the target neuron. For more detailed descriptions of chemogenetic...

  2. AAV Molecular Tools

    Type
    Collection
    ...used for neurophysiology experiments, including neuronal connectivity labeling. ID Name Expression System...TEVp EF1a-driven, Cre-dependent Cre-dependent, bicistronic expression of designer pro-taCasp3 and TEVp for...

  3. Mammalian RNAi Tools

    Type
    Collection
    ...protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design ... in a new window) . Wiznerowicz, M., Szulc, J., Trono, D. (2006). Tuning silence: conditional systems ...

  4. Adenovirus Plasmids

    Type
    Collection
    ...production of viruses containing transgene under Neuron-specific enolase (NSE) promoter Bamburg 50959 ShuttleMCP...pShuttle-CMV-F2A-T2A-Venus Shuttle For production of viruses with multicistronic expression + Venus tag; See plasmid 62622 for...

  5. Retrovirus Plasmids

    Type
    Collection
    ...pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG Envelope VSV-G recommended for the...expression Chen 52114 pMSCV-IRES-mCherry FP MSCV A bicistronic IRES-mCherry containing retroviral plasmid Vignali...

  6. Viral Vectors

    Type
    Collection
    ...Adenoviruses are commonly used as vaccines because of the strong immunogenic response they induce. Some (oncolytic...Community Resources Nolan Lab Retroviral Systems Trono Lab Lentivector Toolbox Guide to Generating Recombinant...

  7. Open Enzyme Collection

    Type
    Collection
    ...polynucleotide kinase 165521 pOpen-lambda red operon lambda red operon 165530 pOpen-T4-BGT T4-BGT beta-glucosyltransferase...

  8. TALEN Plasmids and Kits

    Type
    Collection
    ...of the DNA-binding domain of a TALE, along with strong nuclear localisation tags and repress transcription...transfected cells. TALEN expression is driven by the strong CAG promoter or can be achieved by in vitro mRNA...

  9. CRISPR Plasmids - Tagging

    Type
    Collection
    ...CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed a simple...homology arms. Notably, they have used Auxin-inducible degron (AID) tagging with CRISPR/Cas to generate conditional...

  10. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Gateway recombination-based cloning of complex polycistronic lentiviruses. Can express Cas9 with up to 3 ...in a single vector. sgRNAs are expressed as polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG ...

  14. NETRF

    Type
    Collection
    ...treatment of neuroendocrine cancers. As such, NETRF strongly encourages its funded scientists to make their...

  16. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...homology-directed repair (HDR). HDR is much less error-prone than NHEJ and can be used to faithfully introduce...
Showing: 41 - 60 of 76 results