We narrowed to 73 results for: RON
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TypeCollection...Purification, Cell Ablation, CRISPR Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing...retrograde Retrograde AAV To target projection neurons Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1,...
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Chemogenetics Plasmids
TypeCollection... function and examine the relationship between neuronal signaling and behavior. Depending on the signaling...the opsin to either excite or inhibit the target neuron. LMO can be activated either by adding the luciferin... -
Mammalian RNAi Tools
TypeCollection...protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design ... in a new window) . Wiznerowicz, M., Szulc, J., Trono, D. (2006). Tuning silence: conditional systems ... -
Adenovirus Plasmids
TypeCollection...production of viruses containing transgene under Neuron-specific enolase (NSE) promoter Bamburg 50959 ShuttleMCP...pShuttle-CMV-F2A-T2A-Venus Shuttle For production of viruses with multicistronic expression + Venus tag; See plasmid 62622 for... -
Retrovirus Plasmids
TypeCollection...pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG Envelope VSV-G recommended for the...expression Chen 52114 pMSCV-IRES-mCherry FP MSCV A bicistronic IRES-mCherry containing retroviral plasmid Vignali... -
Open Enzyme Collection
TypeCollection...polynucleotide kinase 165521 pOpen-lambda red operon lambda red operon 165530 pOpen-T4-BGT T4-BGT beta-glucosyltransferase... -
TALEN Plasmids and Kits
TypeCollection...of the DNA-binding domain of a TALE, along with strong nuclear localisation tags and repress transcription...transfected cells. TALEN expression is driven by the strong CAG promoter or can be achieved by in vitro mRNA... -
CRISPR Plasmids - Tagging
TypeCollection...CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed a simple...homology arms. Notably, they have used Auxin-inducible degron (AID) tagging with CRISPR/Cas to generate conditional... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection...events but can improve specificity in targeted neuronal populations. Read our blog post about how to minimize...Collection Optogenetics Plasmid Collection Monosynaptic Neuronal Tracing CRISPR Plasmids and Resources Content ... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...function of your gene of interest in a controlled environment under various conditions. When choosing what ...tag for mammalian expression (Gateway) pYIC - Bicistronic fluorescent reporter gene with cap-dependent ... -
Plan Your Experiment
TypeCollection...Permanently disrupt gene function; cells will use error-prone non-homologous end joining (NHEJ) at the cut site...mutated allele may be different owing to the error-prone nature of NHEJ . In HDR , base editing , or prime... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...Gateway recombination-based cloning of complex polycistronic lentiviruses. Can express Cas9 with up to 3 ...in a single vector. sgRNAs are expressed as polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG ... -
Synthetic Biology - Sensing and Signaling
TypeCollection... related to cell-to-cell communications and environmental sensing. Sensing and Signaling Plasmids Search... -
iPSC Neurodegenerative Disease Initiative Plasmid Collection
TypeCollection...engineering: A new stem cell initiative from the NIH. Neuron . 2021 Apr 7;109(7):1080-1083. PMID: 33831364 (... -
NETRF
TypeCollection...treatment of neuroendocrine cancers. As such, NETRF strongly encourages its funded scientists to make their... -
CRISPR Plasmids - Single-Strand Break (Nick)
TypeCollection...double-strand break (DSB) that is repaired using error-prone non-homologous end joining (NHEJ). Double nicking... -
CRISPR Plasmids - Xenopus
TypeCollection...homology-directed repair (HDR). HDR is much less error-prone than NHEJ and can be used to faithfully introduce... -
CRISPR Plasmids - CRISPR Transposases (CAST)
TypeCollection... almost unidirectional insertions, but are more prone to off-target insertions. CASTs are most often used... -
Bikard Lab - CRISPR Repression Collection
TypeCollection...blocks the progression of the RNA polymerase, thus strongly decreasing the expression level. Mismatches between... -
CRISPR Plasmids - Drosophila
TypeCollection...double-strand break (DSB) that is repaired using error-prone non-homologous end joining (NHEJ). Double nicking...