..., that will probably never happen. Yet with transcription activator-like (TAL) effectors, scientists in...that came out in the spring of 2011, the groups describe a set of customized plasmids that can be used ... a TALEN kit through Addgene. This kit, first described in Nature Biotechnology in August, uses a serial...in Nature Biotechnology early in 2011 and they described their kit for assembling TAL effector arrays in...sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Feb;29(2):149-53. PMID...

...Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes involved in specific... Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled...be fused to a transactivator and used as a transcriptional activator . ID Name Insert Antibiotic Resistance...2A-Blast-WPRE) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version...

...Salvador family WW domain containing protein 1 SCRIB Scribbled planar cell polarity protein SHC SHC1 SHC2 ...specificity phosphatase E2F E2F1 E2F2 E2F3 E2F transcription factor ECT2 Epithelial cell transforming 2 EGFR...protein kinase ETS ETS1 ETS2 ETS proto-oncogene, transcription factor EXOC EXOC1 EXOC2 EXOC3 EXOC4 EXOC5 EXOC6...TBK1 TANK binding kinase 1 TFDP TFDP1 TFDP2 Transcription factor Dp TIAM TIAM1 TIAM2 T-cell lymphoma invasion...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...plasmids and protocols needed for using them, are described below. Read our CRISPR 101 post for more information...CRISPR-Cas tagging system were tested by tagging transcription factors with FLAG in human cell lines. To repeat...targeting vector that encodes both a guide RNA transcript and a repair template for an individual tagging...

...nuclear transcription factor Y, alpha CBF-A, CBF-B, FLJ11236, HAP2, NF-YA NFYB nuclear transcription factor...RAET1G retinoic acid early transcript 1G ULBP5 RAET1L retinoic acid early transcript 1L - RELB v-rel reticuloendotheliosis...endothelin receptor type B ABCDS, ETB, ETBR, ETRB, HSCR, HSCR2 FGF10 fibroblast growth factor 10 - FGF2 fibroblast...endothelin receptor type B ABCDS, ETB, ETBR, ETRB, HSCR, HSCR2 EGF epidermal growth factor (beta-urogastrone...immune response. Over 60 interleukins have been described. NK Cell Signaling Natural killer (NK) cells can...beta CBF-A, CBF-B, HAP3, NF-YB NFYC nuclear transcription factor Y, gamma CBF-C, CBFC, DKFZp667G242, FLJ45775..., PA28G, REG-GAMMA RAET1E retinoic acid early transcript 1E LETAL, MGC125308, MGC125309, RAET1E2, ULBP4...

...zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One 2009) pMLM292 (for expression...zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One 2009) pMLM800 (for expression...Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al. PLoS One 2012 and Cade et al.,...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...Selectable Marker PI Publication Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...PAM = NGG) 46759 pT7-gRNA T7 BsmBI In vitro transcription none, need Cas9 plasmid Chen and Wente 42250...42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung Do you have suggestions for other...

... Glypican Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Integrin... Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse...common cellular proteins. ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse...express plasmid-based antibodies. ID Plasmid Description...

...example, search "Cdc42", "transcription", "Hahn", or other terms. ID Plasmid Description Gene/Insert Vector ...many different types of proteins. For a full description (including peak response spectra) of different...preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication...

... is described in more detail in the Multiplex Assays section below. The following table describes the ...of B cells isolated from animals immunized as described above are used to create hybridomas, which produce...each antibody has two binding sites. Isotype Description Configuration and Valency Subclasses IgA Prevalent...primary antibody for your protein of interest, as described in the IP section above. Reverse the crosslink...validation can be done using methods such as transcriptomics or proteomics. This approach is often appropriate...

... Large collections or kits are described below . ID Plasmid Description Gene/Insert PI Return to top Highlights...that are amenable to genetics, high-throughput screening and non-invasive live imaging. A standard toolbox... Amro Hamdoun Lab. A useful “starter kit” for screening fluorescent protein fusion expression in organisms...

...vivo A common choice for conducting genome-wide screens using CRISPR AAV transduction CRISPR elements are...delivery RNA delivery of Cas9 and gRNA In vitro transcription reactions generate mature Cas9 mRNA and gRNA...complexes Purified Cas9 protein and in vitro transcribed gRNA are combined to form a Cas9-gRNA complex...region from DNA, subcloning into a plasmid, and screening individual clones. PCR amplification and next-...

...with the viral service icon . Metal Ions Target Description Article PI Calcium jGCaMP8 Fast Genetically Encoded...Yan Qin Return to Top Small Molecules Target Description Article PI Acetyl-CoA PancACe acetyl-CoA sensor...Frommer Return to Top Neurotransmitters Target Description Article PI Acetylcholine Family of ACh sensors...Clapham Return to Top Cell Physiology Target Description Article PI Autophagy Measure autophagic flux ...Near-infrared fluorescent voltage indicator nirButterfly Screening and Cellular Characterization of Genetically Encoded...in Living Cells", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type PI Publication Return...

...KLFs) are a family of at least 17 DNA binding transcriptional regulators with diverse roles in processes ...elements or GC-rich regions on DNA to mediate transcriptional activation and/or repression. Continuing collaborative...Addgene's collection of KLF plasmids. ID Plasmid Description Gene/Insert PI...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...encyclopedia of CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

...frequently mutated gene in human cancer, is a transcription factor and tumor suppressor. Wnt Coming soon... Labs containing wild-type and mutant alleles described in the initial Target Accelerator publications... Tackling Cancers’ Drug Resistance with a New Screening Kit (David Sabatini & Kris Wood) National Cancer...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...encyclopedia of CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...direct dCas9 to a specific locus, avoiding known transcription factor and other protein binding sites. Browse...