We narrowed to 79 results for: Tes

...Brzezinski Lab CRISPR Collection The Brzezinski lab investigates gene regulation in the context of the developing...fusion shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2...

...oxidizes its substrate (luciferin), it generates light that activates the opsin to either excite or inhibit...

...proteins to label and detect multiple injection sites in the same tissue, as well as barcoding with specific...Stochastic infection of pooled AAVs delivered in vivo generates unique combinations of protein bits. B) Multiplex...

...contains pre-assembled genetic circuits such as logic gates and higher level gene networks. Sensing and Signaling...Quorum Sensing Ellis Lab GeneGuard Endy Lab Logic Gates , BIOFAB Promoter/BCD Kit , and pOSIP Plasmid Kit...

... length of the spacer sequence between the half-sites (see Nuclease Expression Vectors for more details...two-hybrid reporter system but have NOT yet been tested for activity as ZFNs in zebrafish. Target Gene ...

...microbial fields, including bacteria, viruses, parasites such as protozoa, and fungi. Find plasmids below...microbiology resources. The plasmids Addgene distributes cannot be used to reconstitute self-replicating...

...set of plasmids for building homologous repair templates that incorporate a self-excising drug selection...Center (CGC) - The CGC collects, maintains, and distributes stocks of C. elegans. Silencing Genomes - Cold...

...transfer vectors you can find reporter plasmids to test whether or not you've efficiently generated infectious...recombinases to remove genes flanked by either loxP or FRT sites respectively, and empty vectors to express your ...

...window) PMID: 10508514 2007 - Adrian Bird's lab demonstrates that Rett syndrome symptoms are reversible in...Joost Gribnau Xist 2lox/2lox Conditional Xist, Lox sites flanking exon 1,2,3 C57BL/6 Mouse line with conditional...

...untranslated regions, or shRNA or CRISPR target sites can be inserted into a luciferase-containing plasmid..., and different luciferases require different substrates and detection wavelengths. Firefly luciferase...

...staining, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector ...vector preparation. In some cases, in vivo testing of these viral preparations was performed by the Karpova...

... and reversible control of gene expression in eukaryotes. To avoid secondary effects from repurposing ...tetracycline, tTA binds to the TRE and its VP16 domain promotes gene expression. In the presence of tetracycline...

... represent the official views of the National Institutes of Health....

...and terminators repressors and activators logic gates Networks and Gene Regulation Plasmids Search the...

...fused to FokI nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence...

... Antibodies Addgene distributes ready-to-use recombinant antibodies to help facilitate...

...adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts adenosine...

...in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease domains...

...for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential for regulating...

...plasmids from the Toronto, Oxford and Stockholm sites have been deposited at Addgene and are available...