We narrowed to 87 results for: mpl
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TypeCollection...interacts only with their own target sites (for example, Cre with lox sites, Flp with FRT sites, Dre with...preferred codon usage will improve expression. For example, iCre or FLPo in mammalian cells may facilitate...translocation of a gene of interest. Using Cre or Flp as examples, here are a few popular recombinase-dependent ...
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COVID-19 Resources
TypeCollection...virus Mammalian constructs Due to the size and complexity of the collection, all available Ginkgo SARS-...top General Plasmid Tools SHERLOCK and DETECTR - Simple, portable, and inexpensive platforms to reliably...Andrew Ellington's lab. High-surety isothermal amplification and detection of SARS-CoV-2, including with ... -
Rett Syndrome
TypeCollection...methylated DNA and the recruitment of the NCoR complex for proper nervous system function. While there...expressing Cas9 and empty gRNA backbones. A few examples of additional CRISPR resources can be found below...cell line repository (Link opens in a new window) Sampled - one of the world's largest biorepositories (Link... -
Bacterial Expression Systems
TypeCollection...transfer (FRET) and bimolecular fluorescence complementation (BiFC) are techniques that have been widely...compatible vectors for bimolecular fluorescence complementation experiments in plants. The Zinc Finger Consortium...Introduction to FRET Bimolecular Fluorescence Complementation Makes Visualizing Protein–Protein Interactions... -
CRISPR Pooled gRNA Libraries
TypeCollection...using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic locus...screening experiments require electroporation to amplify the pooled library and the use of Next-Generation...3rd 6 12,727 Yeast Inducible CRISPRi Library – Ampl133 161829 Inhibition Yeast Tavazoie N/A 6-12 51,681... -
Plasmids for Stem Cell Research
TypeCollection...generate iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem...and Xeno-Free Culture Conditions: A Clinically Compliant Approach. Stem Cells Transl Med. 2015 Mar 5. pii...Lentiviral Mouse Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell... -
Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection
TypeCollection...factor isoform has a unique 24 bp barcode that simplifies identification of the transcription factor isoform...is uniquely barcoded with a 24 bp sequence, to simplify identification during screening. Library ID PI... -
CRISPR Plasmids - Xenopus
TypeCollection...possibly resulting in frameshifts. If a repair template with high homology to the DNA surrounding the ...Cas9 species = S. pyogenes (PAM = NGG) 51306 pUC57-Simple-gRNA backbone T7 BsaI In vitro transcription none... -
AAV Viral Preps
TypeCollection...Rabies Virus-Based Serotype Serotype Testing Samples Small samples of control AAV vectors for trial purposes... -
CRISPR Plasmids - Double-Strand Break (Cut)
TypeCollection...changes, researchers use ssDNA or dsDNA repair templates with 1. homology to the DNA flanking the DSB and...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed... -
CRISPR Plasmids - Zebrafish
TypeCollection...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed... -
CRISPR Plasmids - Parasites
TypeCollection...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed... -
CRISPR Plasmids - CRISPR Transposases (CAST)
TypeCollection...Cas enzymes that form a complex called Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense... -
DNA Service - Cloning Grade DNA
TypeCollection...reducing the time to experiments by removing the amplification and extraction steps required when one receives...through our website. Every order will need to be completed independently including the check out and will... -
Optogenetics Plasmid Collection
TypeCollection...plasmids for neuronal excitation and inhibition. For example, search for "ChR mCherry", "Cre inhibition", or...to control a protein or cellular activity. For example, search "Cdc42", "transcription", or other terms... -
Viral Prep Service for Plasmids and Pooled Libraries
TypeCollection...Our production process and quality control, with examples of quality control data. Help Center Articles ...will also include virus-associated DNA, which is a sample of the purified DNA from the original plasmid that... -
SARS-CoV-2 Pseudotyped Virus
TypeCollection...the SARS-CoV-2 Spike protein and you will find examples of each in Addgene's collection: HIV-based lentiviral...and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for measuring... -
CRISPR Plasmids - C. elegans
TypeCollection...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed... -
Addgene Packaged on Request: Scope of Service
TypeCollection... sequence verification, may not yet have been completed at the time of shipment. If any discrepancies ...for any additional customs fees or taxes, and to complete any additional customs documentation that might... -
New England Biolabs Cell-Imaging Plasmid Collection
TypeCollection...allow for dual labeling and visualization of complementary protein function or localization. ACP and MCP...permitting selective simultaneous labeling in a sample. Please see the note below for information on substrates...