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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 125 results for: ANT

Showing: 61 - 80 of 125 results

  1. Retrovirus Plasmids

    Type
    Collection
    ... genome into a host cell. Scientists can take advantage of this to stably integrate transgenes of interest...Guide Popular Gamma-Retroviral Transfer Plasmids Want a retroviral plasmid with your gene of interest?...pBABE-neo largeTcDNA MoMLV Expression of Large T antigen for creation of immortalized cells. See the Weinberg...Weinberg Lab page for additional resistance variants. Bob Weinberg 1773 pBABE-hygro-hTERT MoMLV Expression...

  2. mTOR Pathway

    Type
    Collection
    ...Loss of the tumor suppressor p53 also promotes aberrant mTORC1 activity, and multiple familial cancers...uncontrolled cell growth and proliferation, including an abundant supply of macromolecules and energy, as well as... as the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears to signal... mTOR signaling in growth control and disease. Laplante M, Sabatini DM. Cell. 2012 Apr 13;149(2):274-93...

  3. AAV Packaged on Request

    Type
    Collection
    ...though actual yields may vary by construct. Size Quantity Total Volume Minimum Titer Typical Titer Small... serotypes: AAV1 AAV2 AAV5 AAV6 AAV8 AAV11 AAVrg Want more information before choosing a serotype? Learn... on Request preps is all-inclusive of several important steps: MTA facilitation for the transfer plasmid...you need more assistance with troubleshooting, we want to hear from you! Email us at [email protected] ....

  4. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...originally discovered as contaminants of adenovirus stocks. One major advantage of using AAV for research...low immune response and low toxicity. Although recombinant AAV does not integrate into the host genome, ...AAV Viral integration AAV serotypes Pseudotyping Want to save time? Check out our Viral Vector Packaging...

  5. Lentiviral Prep Service

    Type
    Collection
    ...single-stranded breaks ( nicks ). ID Name Insert Function Antibiotic Resistance Activity PI 52962 lentiCas9-Blast ... a transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9... express fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446 pLenti CMV GFP Hygro... in a new window) . Please note this does not guarantee viral service, but lets us know what viruses would...

  6. Zebrafish Plasmid Collection

    Type
    Collection
    ...during Development and Regeneration - Periklis Pantazis Lab Multibow: digital spectral barcodes for cell... loss and ablation of previously resistant cell types. CreLite - George Eisenhoffer Lab. ...for Zebrafish Receptors and Zebrafish Monoclonal Antibody plasmids - Gavin Wright Lab. Collections of plasmids...that encode zebrafish receptors or monoclonal antibodies to zebrafish cell surface receptors and secreted...

  7. Viral Production

    Type
    Collection
    ...viral genome and the absence of unexpected DNA contaminants. The data is also used to determine recombination...properties of the final vector preparation, the quantity of gram-negative bacterial endotoxin is ensured...within representative vector preparations was quantified with electron microscopy after negative staining...Approximately 2 weeks post-thaw, cell culture supernatant is tested for mycoplasma contamination. To date...

  8. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...available as cgDNA, we hope to aid scientists who want to immediately start cloning upon arrival of their...determined by spectrophotometer. Other methods of quantification may yield slightly different DNA concentrations...Suggestion Form . Please note that this does not guarantee that Addgene will make cloning grade DNA preps...

  9. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...gene targets. To accomplish this they have taken advantage of the Zhang lab all-in-one (guide/Cas9/reporter... with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. ...

  10. Resolute Plasmid Collection

    Type
    Collection
    ... Medicines Initiative 2 Joint Undertaking under grant agreement No 777372. This Joint Undertaking receives... production is used for assays and to generate antibody/ high-affinity ligands. Data is embedded in an...knowledgebase that features, among others, genetic variants and disease associations. Plasmids This plasmid...

  11. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...-type malate dehydrogenase genes and watermelon mutant clones for your course-based undergraduate research...single protein with many available isoforms and mutants provides a shared experience for the research community...of wild-type MDH genes from several species and mutant clones from watermelon have been codon-optimized...

  12. E11 Bio PRISM Collection

    Type
    Collection
    ... of stochastic combinations of cell-filling antigenically distinct protein tags (or "bits"), creating ...over 100,000 unique combinations detectable by antibodies. All of the PRISM protein tags have been validated...corresponding primary and dye-conjugated secondary antibody (as listed in Park & Sheridan et al., 2025). These...

  13. Microbiology Resources

    Type
    Collection
    ...See below for plasmid kits and other resources relevant to these organisms, or use our Search page to ...Creating three-part fusion proteins - Planas and Santalucia Labs pCri System : Heterologous protein expression...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...

  14. Promega Plasmid Collection

    Type
    Collection
    ...bioluminescent assays or with the high-affinity anti-HiBiT antibody for additional detection options. HiBiT technology's...intracellular and extracellular applications where you want minimal disruption of endogenous protein expression...

  15. TALEN Guide

    Type
    Collection
    ...effectors, which were isolated from the plant bacterial pathogen Xanthomonas. The central TAL targeting domain...plasmid toolbox. Addgene has rapidly become an important resource for this new technology, and we hope ...

  16. TALEN Plasmids and Kits

    Type
    Collection
    ...transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind...Guide CRISPR Plasmids Zinc Finger Kits TALENs are important new tools for genome engineering. Fusions of transcription...

  17. Caltech Systemic Capsids

    Type
    Collection
    ... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...the best capsid for your cell type. First, do you want to target CNS or PNS? Then consider species...species, and then cell type. For CNS: For infant rhesus macaques: Broad tropism - AAV.MaCPNS1, AAV.MaCPNS2...

  18. Biosensor AAV Preps

    Type
    Collection
    ...Calcium Sensor: GCaMP7c 105321 pGP-AAV-syn-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Constitutive 1 Kim ...Kim 105322 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Cre 1 Kim Calcium Sensor: GCaMP6f... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

  19. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation...created a number of plasmids for expression of variants of LRRK2 , SNCA , and other proteins of interest...

  20. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ... gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources...Like nickase technology, this technique reduces unwanted off-target effects of CRISPR. Browse, sort, or...
Showing: 61 - 80 of 125 results