We narrowed to 116 results for: ANT

...gene targets. To accomplish this they have taken advantage of the Zhang lab all-in-one (guide/Cas9/reporter... with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. ...

...-type malate dehydrogenase genes and watermelon mutant clones for your course-based undergraduate research...single protein with many available isoforms and mutants provides a shared experience for the research community...of wild-type MDH genes from several species and mutant clones from watermelon have been codon-optimized...

...See below for plasmid kits and other resources relevant to these organisms, or use our Search page to ...Creating three-part fusion proteins - Planas and Santalucia Labs pCri System : Heterologous protein expression...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...

...bioluminescent assays or with the high-affinity anti-HiBiT antibody for additional detection options. HiBiT technology's...intracellular and extracellular applications where you want minimal disruption of endogenous protein expression...

...effectors, which were isolated from the plant bacterial pathogen Xanthomonas. The central TAL targeting domain...plasmid toolbox. Addgene has rapidly become an important resource for this new technology, and we hope ...

...gene therapy development. In order to produce recombinant AAV vectors, researchers need to use three plasmids...viral production, and viral safety. AAV Vector Guide Want to save time? Our Viral Vector Packaging Service...cycle. To avoid the use of these viruses during recombinant AAV vector production, necessary helper virus...

...transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind...Guide CRISPR Plasmids Zinc Finger Kits TALENs are important new tools for genome engineering. Fusions of transcription...

... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...the best capsid for your cell type. First, do you want to target CNS or PNS? Then consider species...species, and then cell type. For CNS: For infant rhesus macaques: Broad tropism - AAV.MaCPNS1, AAV.MaCPNS2...

...Calcium Sensor: GCaMP7c 105321 pGP-AAV-syn-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Constitutive 1 Douglas...Douglas Kim 105322 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Cre 1 Douglas Kim Calcium... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation...created a number of plasmids for expression of variants of LRRK2 , SNCA , and other proteins of interest...

...Vectors By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources...Like nickase technology, this technique reduces unwanted off-target effects of CRISPR. Browse, sort, or...

...to generate and characterize single nucleotide variant lines in an isogenic cellular background. All cell...create cell lines with endogenously-tagged gene variants or to differentiate human iPSCs into other cell...

...Interfere CRISPR Plasmids: Interfere Mammalian Bacteria Plant Yeast Resources Catalytically dead Cas9 (dCas9),...Gene/Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...

...Plasmids: Activate Mammalian Bacteria Drosophila Plant C. elegans Yeast Resources Catalytically dead dCas9...Gene/Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...

...Epigenetics CRISPR Plasmids: Epigenetics Mammalian Plant Resources To make targeted epigenetic modifications... Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...

...CRISPR Plasmids: RNA Targeting Mammalian Bacteria Plant Resources Type VI CRISPR systems, including the ...Plasmid Gene/Insert PI Publication Return to top Plant ID Plasmid Gene/Insert PI Publication Return to ...

...tags, can be used for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved ...

...CRISPR Plasmids: Cut Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus Parasite Resources... Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...

...γ-Retrovirus Adenovirus All Viral Plasmids Curated Topics Antibody Plasmid Collection Bacterial Expression Systems...Expansion Immunology Mammalian RNAi Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible...

...used to engineer ZFNs that function in zebrafish, plants, and human cells...used to engineer ZFNs that function in zebrafish, plants, and human cells ( Maeder et al., Mol Cell 2008...