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We narrowed to 80 results for: Cre expression vector

Showing: 61 - 80 of 80 results

  1. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...getting all components of the Cascade complex to express equally. To combat this, some CRISPR-Cas3 plasmids...Cascade-Cas3 plasmids. Plasmids are available for expression in mammalian systems, bacteria, and plants. Mammalian...to deletions generated by Cas9, large deletions created using Cas3 have fewer off-target alterations and... Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse, sort, or search the...

  2. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...genes encoded by 3,548 isoforms and two control vectors. The individual clones, or the pooled library containing...factor isoform in pooled screens. This collection and library can be used for screening transcription factors...available at Addgene. The collection can be used for screening transcription factors for cellular programming...Depositor Collections MORF Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection You...window) . TF ORF barcode PCR for NGS QC and bulk screening (PDF, 85 KB) TF ORF barcode dial out PCR for 10X...bp sequence, to simplify identification during screening. Library ID PI Description MORF library 137000...

  3. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...gRNA expression vectors available from Addgene....Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for...activity. The variety in sequence decreases the gRNA activity and increases off-target effect. Which species...requirements, experimental considerations, and more, created and maintained by the Doudna Lab . gRNA Plasmids...

  4. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...codon-optimized for expression in bacteria and cloned into an IPTG-inducible His-tag expression vector. All His-tags...sustainable MDH-related CURE focusing on protein expression, structure and function, kinetics and mechanisms.... PMID: 36893201 (Link opens in a new window) Increasing access for biochemistry research in undergraduate...

  5. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system...purify genomic loci. Plasmids are available for expression in mammalian systems, bacteria, and yeast. Mammalian...

  6. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...Further advancements have been made by optimizing expression of the fusions, modifying the linker region between... editing plasmids. Plasmids are available for expression in mammalian systems, bacteria, plants, yeast...double stranded breaks. Cytosine base editors are created by fusing Cas9 nickase or catalytically inactive...converted to thymidine through base excision repair, creating a C to T change (or a G to A on the opposite strand..., which is treated like guanosine by the cell, creating an A to G (or T to C) change. Adenine DNA deaminases...deaminases do not exist in nature, but have been created by directed evolution of the Escherichia coli TadA...

  7. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Y., Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection markers for...Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with yeast...yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al...FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007) containing the PMA1 promoter and...blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902. https...Marker Tags Publication FP Overexpression Plasmids for constitutive overexpression of codon-optimized FPs ...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...

  8. CRISPR Guide

    Type
    Collection
    ...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects... gRNAs will be expressed in the same cell. This is called multiplexing , and increases the likelihood ...lentivirus or use a Cas9-expressing stable cell line. After applying your screening conditions to your transduced...also been used for genome-wide screens to activate or repress gene expression in mice and human cells. The...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...

  9. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR-FokI plasmids. Plasmids are available for expression in mammalian systems and Drosophila. Mammalian...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...

  10. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... by expressing and purifying the phosphosite library as GST-fusion peptides (“Mode #1” expression). The...can also be introduced into a second expression vector encoding a split mCherry fluorescent reporter, ...characteristics, and an overall improved ease of use when expressing recombinant phosphoproteins. These attributes... origins. Therefore, when choosing plasmids to express the phosphoprotein of interest, a medium copy RSF1030...2023) and find general tips on phosphoprotein expression in rEcoli XpS in the Methods for iSPI, rEcoli...phosphorylation-dependent protein-peptide interactions (“Mode #2” expression, Barber et al., 2018). The phosphosite library...at the central position. Introducing Hi-P for screening phosphorylation-dependent protein-protein interactions...

  11. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... nickase plasmids. Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

  12. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...full sequencing of the final viral vector preparation. These viral vector preparations were produced with...rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep... Viral Vector Packaging Service AAV University of Florida Serotype...Serotype Testing Panel for the Eye and Brain Viral Vector Packaging Service: University of Florida Serotype...Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part of our Viral Service , Addgene is producing...of our standard viral production , these viral vectors undergo quality control, including AAV titration... in vitro as well as in vivo delivery where the vector is diluted by the physiologic body fluids, such...

  13. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...targeting plasmids. Plasmids are available for expression in mammalian systems, bacteria, and plants. Mammalian...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

  14. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR expression plasmids that have been designed for use in parasites. CRISPR... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...

  15. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...to introduce a DSB. Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants...CRISPR plasmids that create double strand breaks in DNA. CRISPR... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

  16. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...Transposase plasmids. Plasmids are available for expression in mammalian systems and bacteria. Mammalian ...Figure 1: Overview of the basic CAST mechanism. Created with BioRender.com. Browse, sort, or search the...

  17. Genetic Code Expansion

    Type
    Collection
    ...plasmids, as depicted in the figure below: A plasmid expressing the tRNA and its cognate aminoacyl-tRNA-synthetase...codon is often used. The gene of interest can be expressed from a plasmid containing a UAG codon at the place...with a different amino acid. They are typically expressed from a single plasmid, with multiple copies of...make sure that these conditions are true. Always express first with a control reporter gene – GFP for E....mCherry-GFP for mammalian cells. You should also express the protein with and without the ncAA in the media... tRNA gene. ID Plasmid Synthetase Origin ncAA Expression Codon PI 31186 pEVOL-pAzF p-azidohenylalanine...mutated, serB deleted for phosphoserine GCE. For expressing phosphoserine-containing proteins using genetic...

  18. Fluorescent Proteins: FRET

    Type
    Collection
    ...3.1 pmTurquoise2-N1 , pCS2+mNeonGreen-C Cloning Vector , mNeonGreen-mTurquoise2 CyPet YPet 435 0.51 530... 128,000 0.45 6.4 4.7 pCS2+mNeonGreen-C Cloning Vector , pcDNA3-mRuby2 , mNeonGreen-mRuby2-FRET-10 SYFP2...fluorophores through linkers, fluorescent protein expression level and maturation time, and other environmental...imaging microscopy (FLIM) or FLIM-FRET, where the increased energy transfer through FRET reduces the donor...

  19. Resolute Plasmid Collection

    Type
    Collection
    ... SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors allow a fast and efficient sub-cloning...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression pooled .... Human SLC Overexpression Library 213694 Superti-Furga Inducible barcoded overexpression library of human...pooled libraries are useful tools for genetic screening experiments. These libraries target the solute carrier...

  20. Immunology Research Plasmids and Resources

    Type
    Collection
    ...® Entry vectors from the labs of William Hahn and David Root . Multiple Lentiviral Expression (MuLE) System...finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced by B cells ...factor (TNF) family of proteins are predominantly expressed by immune cells and help to regulate the immune...regulatory factor X, 5 (influences HLA class II expression) - RFXANK regulatory factor X-associated ankyrin-containing...systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites and standardized building...selection cassettes and tools for inducible gene expression. Myristoylated Kinase Kit : Consists of 190 human...
Showing: 61 - 80 of 80 results