We narrowed to 75 results for: cas
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TypeCollection...deletion in E3 and insertion of GFP expression cassette. Bunz 179202 pAd5-B6/7 ∆E3-GFP Adenoviral A block...deletion in E1 and insertion of GFP expression cassette. Bunz 16402 pShuttle Shuttle For production of...Shuttle Modified pShuttle-CMV plasmid with Gateway cassette; See article for additional plasmids Zhang 50957...
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Antibody Guide
TypeCollection...expression. Monoclonal antibodies are necessary in cases where extreme specificity is important. Their epitope...when epitope specificity is not a concern or in cases where you may want to identify or collect the protein...azide, as it acts as an inhibitor to HRP. In those cases, thimerosal can be used as an antimicrobial agent... using the above process and try again. In some cases, it may be preferable to buy small aliquots of many... -
Bacterial Expression Systems
TypeCollection...protein expression in the stationary phase. In these cases, you may want to control protein levels or to turn...Plasmid Promoter Inducer Expression Species PI 44249 pdCas9-bacteria pTetO Anhydrotetracycline (aTc) Escherichia...synthesize a target metabolite, genetic tools like CRISPR-Cas9 are not only valuable but often essential. Find ... -
Plasmids for Stem Cell Research
TypeCollection...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes. piggyBac transposition reprograms fibroblasts...CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice... -
Brain Initiative Collection
TypeCollection...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV8 pAAV-Ef1a-DIO-PPO-Venus...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV9 pAAV-Ef1a-DIO-PPO-Venus...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 140135-AAV1 pAAV-EF1a-iCreV can... -
Addgene Packaged on Request: Scope of Service
TypeCollection...titered by droplet digital PCR (ddPCR) . In some cases, we may expect lower titers for a particular AAV...titer due to toxicity or other reasons For these cases, Addgene will promptly notify you by email within... -
TALENs for Endogenous Zebrafish Genes
TypeCollection...Site (5' to 3'; half-sites in CAPS, spacer in lowercase) aak1 TAL3208 & TAL3209 TCTTTGACTCCCGGAGAGagctggtgggctccgggCCAGGTTCTGGAGGGGGA...TGGACGCGGAGCTGTTCTcggagcaggagctgacCTGCTCCATCTGTCTGGA casp8ap2 TAL3038 & TAL3039 TATAACAAATCCCACCACattgcccgatatcttgAATACTTCCAAAACAAAA... -
Zinc Finger Consortium: Nuclease Expression Vectors
TypeCollection...mammalian cells or zebrafish). pMG10 (a '“2-in-1” ZFN cassette for expression of both a “+” and “-” heterodimeric... -
Distribution to Industry
TypeCollection...Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome editing of human TP53... -
Arf GTPase Family
TypeCollection...Link opens in a new window) Sztul, E., Chen, P., Casanova, J. E., Cherfils, J., Dacks, J. B., Lambright,... -
Viral Production
TypeCollection... contamination. To date, Addgene has never had a case of mycoplasma contamination. In the event of contamination... -
Ras Pathway
TypeCollection...and differentiation. Schematic of Ras signaling cascade that starts at the plasma membrane. Cancer Impact... -
TALEN Plasmids and Kits
TypeCollection... 21179091), as well as (v) distinct selection cassettes on pTAL7a and pTAL7b for enrichment of double-... -
Retrograde AAV viral preps
TypeCollection... of the final viral vector preparation. In some cases, in vivo testing of these viral preparations was... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection...expression of GFP and/or mCherry, with neomycin cassette Zebrafish, Sea urchin, Xenopus, and C. elegans...