We narrowed to 92 results for: nin

...directions from the target region, effectively turning Cas3 into a DNA “shredder” system that can cause... a boundary defined by anti-CRISPR proteins or running into other proteins bound to DNA, and the break...

...Cui, L., Bikard, D., & Van Teeffelen, S. (2018). Tuning dCas9’s ability to block transcription enables ...Dodd, I. B., & Shearwin, K. E. (2013). One-Step cloning and chromosomal integration of DNA. ACS Synthetic...

...background information on CRISPR and the basics for planning your first CRISPR experiment. CRISPR 101 eBook...libraries are a powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used...

...window) External Collaboration Results in Student Learning Gains and Positive STEM Attitudes in CUREs. Callahan...undergraduate research experiences (CURE) impacts student learning and attitudinal outcomes: A study of the Malate...

...Technology SNAP-tag SNAP-tag is based on the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein... but reacts with benzylcytosine rather than benzylguanine derivatives to allow for dual labeling and visualization...

...sensitive gene expression analysis and fusion vectors containing tags such as NanoLuc, HaloTag, SmBiT, and LgBiT...editing outcomes, and conducting high-throughput screening in drug discovery. NanoLuc Fusions NanoLuc (Link...

...PRNP Dementia Susan Lindquist 22291 pcDNA3-FLAG-Synaptojanin 1-145 SYNJ1 Flag CMV Parkinsonism, early-onset...early-onset Pietro De Camilli 22292 pcDNA3-FLAG-Synaptojanin 1-170 SYNJ1 Flag CMV Parkinsonism, early-onset Pietro... Pietro De Camilli 22293 pEGFPC1-FLAG-Synaptojanin 1-145 SYNJ1 GFP, Flag CMV Parkinsonism, early-onset...early-onset Pietro De Camilli 22294 pEGFPC1-FLAG-Synaptojanin 1-170 SYNJ1 GFP, Flag CMV Parkinsonism, early-onset... U6 Parkinson's Timothy Ryan 223563 pmCherry-Synaptojanin-1-145 SYNJ1 His, mCherry CMV Parkinsonism, early-onset...Parkinson's, FTD Andrew Steele 241303 mVenus-synaptojanin1(498-901)-CAAX SYNJ1 mVenus CMV Parkinsonism,...Parkinsonism, early-onset Justin Taraska 241304 mVenus-synaptojanin1(D730A,498-901)-CAAX SYNJ1 mVenus CMV Parkinsonism...

...Sensors dLight RdLight GRAB_DA GRAB_rDA GRAB_gDA Serotonin Sensors psychLight iSeroSnFR GABA Sensors iGABASnFR... Syn GRAB_gDA3m none Constitutive 1 Yulong Li Serotonin Sensors 128485 pAAV-CAG-iSeroSnFR-Nlgn CAG iSeroSnFR...

...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

... Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease...

...gene of interest’s promoter, enhancer, or the beginning of the coding sequence. Figure 1: Overview of ...

...cancers. The NETRF believes that creating and maintaining a highly collaborative NET research community...

...Find fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab....

...Rinehart FreeGenes Project Open Enzyme Collection Screening Multiplexed Overexpression of Regulatory Factors...

... in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease...

... repaired using error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted...

...CRISPR function, but require an additional plasmid containing a Cas enzyme or cells or organisms in which Cas...