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We narrowed to 106 results for: tro

Showing: 61 - 80 of 106 results

  1. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ... vectors undergo quality control, including AAV titration by ddPCR, in vitro and (when possible) in vivo...chloride, 20 mM Tris at pH 8.0) Suitable for in vitro as well as in vivo delivery where the vector is ...pTR-UF11 chimeric CMV/Chicken Beta actin (CBA) GFP Control Sergei Zolotukhin Citation Information AAV2(Y444F...

  2. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...phosphoserine in >36,000 phosphosites by tandem mass spectrometry (Barber et al, Nat. Biotech. 2018). This phosphosite...supD tRNA ( 68307 ) at the central position. Introducing Hi-P for screening phosphorylation-dependent ...interactions. The phosphosite DNA library can also be introduced into a second expression vector encoding a split...human substrates of kinases of interest by mass spectrometry in a technique called serine-oriented library...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...recombinase, and Kras G12D HDR template 60225 : control for AAV-KPL; sgRNA targeted to LacZ, plus luciferase...within the mouse genome. This plasmid is used as a control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone...targeting the neuronal-specific gene NeuN. As a control they designed an sgRNA targeting LacZ, which is... L, Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33...

  4. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ... 1,836 genes encoded by 3,548 isoforms and two control vectors. The individual clones, or the pooled library...Abudayyeh OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell 2023 Jan 5; 186(1)...reading frames with or without GFP and mCherry controls. Do you have suggestions for other plasmids that...

  5. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Posts Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location...frameshifts, creating loss of function alleles. To introduce specific genomic changes, researchers use ssDNA... tables below for CRISPR plasmids designed to introduce a DSB. Plasmids are available for expression in...

  6. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...find current pricing information Standard Quality Control Plasmid verified by next generation sequencing ...Plasmid Description Industry PI FAQ What Quality Control analyses are performed on my cloning grade DNA ... and 260/280 ratio has been determined by spectrophotometer. Other methods of quantification may yield...

  7. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...blocks the progression of the RNA polymerase, thus strongly decreasing the expression level. Mismatches between...target sequence lower the repression level. By controlling the number of mismatches we can obtain a range...reporters, mCherry and sfgfp in this case, can be controlled using a plasmid‐borne CRISPR array coding for...

  8. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...production process and quality control, with examples of quality control data. Help Center Articles Additional...various aspects of viral vectors, including troubleshooting advice and explanations of viral vector technologies...

  9. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Filaments Utrophin (aa# 1-261) GFP William Bement 26740 mCherry-UtrCH Actin Filaments Utrophin mCherry ...cycle) Cep170 EGFP Erich Nigg 41163 pEGFP PICH Centromeres (dependent on cell cycle) PICH EGFP Erich Nigg...EGFP Ken-Ichi Takemaru 54234 mEmerald-PLK1-N-16 Centrosome Plk1 mEmerald* Michael Davidson 54491 mCherry-Lifeact...

  10. Mammalian RNAi Tools

    Type
    Collection
    ...plasmids are in a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive...protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design ... in a new window) . Wiznerowicz, M., Szulc, J., Trono, D. (2006). Tuning silence: conditional systems ...

  11. mTOR Pathway

    Type
    Collection
    ...apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation. mTOR, a serine...in cancer promotes the conditions needed for uncontrolled cell growth and proliferation, including an ...Kris Wood) References mTOR signaling in growth control and disease. Laplante M, Sabatini DM. Cell. 2012...

  12. Worm Expression Resources

    Type
    Collection
    ...CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR ...silencing in the germline. A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. - ...Hubbard Lab. Plasmids for the temporal and spatial control of gene expression in C. elegans by combining expression...

  13. Rett Syndrome

    Type
    Collection
    ...the location and type of MECP2 mutation can be a strong predictor of symptom severity. Broadly, early gene... in a new window) RSRT iPSC lines and isogenic controls , patient-specific Infinity BiologiX - RSRT cell...refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR...

  14. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...mind: CRISPR screening experiments require electroporation to amplify the pooled library and the use of...GBC) 85968 Barcode Human Weissman 3rd N/A N/A Retroviral Mouse Genome-wide CRISPR Knockout Library 104861...104861 Knockout Mouse Teichmann N/A (retroviral) 5 90,230 RNA-Binding Protein Pooled CRISPR Knockout Library...

  15. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...pHIV-Zsgreen - Mammalian Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1...- Mammalian Expression pMXs-mRFP1 - Mammalian Retroviral Expression mCherry 587 610 16 4.5 15 min Monomer...proteins Insect Sutherland Lab Plasmids - For multicistronic expression of GFP and/or mCherry, with neomycin...

  16. Antibodies

    Type
    Collection
    ...application-specific validation and consistent quality control measures. We work with trusted partners and experts... by sharing our protocols and quality control methods. Learn More Guide New to using antibodies...

  17. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ... CRISPR Blog Posts CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead...mutants can also be used with a repair template to introduce specific edits via homology-directed repair (HDR...

  18. CRISPR Plasmids - Parasites

    Type
    Collection
    ... Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location...frameshifts, creating loss of function alleles. To introduce specific genomic changes, researchers use ssDNA...

  19. AAV Molecular Tools

    Type
    Collection
    ... These AAV encode tetracycline-inducible tools/controls and tetracycline transactivators that can be used...TEVp EF1a-driven, Cre-dependent Cre-dependent, bicistronic expression of designer pro-taCasp3 and TEVp for...

  20. Adenovirus Plasmids

    Type
    Collection
    ...100% efficiency. Unlike lentiviruses or other retroviruses , adenoviruses do not integrate into the host...pShuttle-CMV-F2A-T2A-Venus Shuttle For production of viruses with multicistronic expression + Venus tag; See plasmid 62622 for...
Showing: 61 - 80 of 106 results