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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 123 results for: tra

Showing: 81 - 100 of 123 results

  1. mTOR Pathway

    Type
    Collection
    ... mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation factor 4E, binding protein 1 Cyclin...Cyclin D1 Also known as CCND1 eIF-4E Eukaryotic translation initiation factor 4E GBL/mLST8 MTOR associated...Neurofibromin 1 PRAS40 Also known as AKT1S1; AKT1 substrate 1 (proline rich) PTEN Phosphatase and tensin homolog....2014.01.001. PubMed PMID: 24508508 . Targeting the translation machinery in cancer. Bhat M, Robichaud N, Hulea...

  2. Microbiology Resources

    Type
    Collection
    ...plasmids is a bacterial stab (of a laboratory E. coli strain). Addgene continues to monitor its library and ... Integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica ...Restoration of prototrophy in common S. cerevisiae lab strains - Ralser Lab Return to top Resources for... in bacterial genome editing is not always straightforward. Read our CRISPR Methods for Bacterial Genome...

  3. Lentiviral Prep Service

    Type
    Collection
    ... used to generate double-stranded breaks ( DSBs or cuts ) or single-stranded breaks ( nicks ). ID Name...Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional activator . ID Name Insert Antibiotic...

  4. Retrograde AAV viral preps

    Type
    Collection
    ...this serotype to deliver Cre-dependent transgenes in Cre transgenic lines. As part of our standard viral...serotype permits robust retrograde access and transgene delivery to projection neurons. Specific classes...titering by SYBR green qPCR , confirmation of the transfer plasmid with PCR, purity assessment by silver ...

  5. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs)....Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence. Two unique ...

  6. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...CRISPR plasmids for transcriptional repression of target genes....Catalytically dead Cas9 (dCas9), or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. This interference is most efficient when the gRNA...

  7. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...functional Cas9 enzymes designed to introduce a double-strand break (DSBs) at a specific location based on a ...pUC57-Simple-gRNA backbone T7 BsaI In vitro transcription none, need Cas9 plasmid Chen Do you have suggestions...

  8. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...pinpoint genes linked to parasite virulence, transmission, and development. This collection brings together...functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...

  9. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...technical reports from the research community demonstrating AAV performance under various experimental conditions... vectors from the Penn Vector Core have been transferred to Addgene for distribution. Look up the IDs ...actual material being referenced in the Material Transfer Agreement (MTA) and its inclusion is what gives...

  10. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...reasons: Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids ... Terms of Purchase and the applicable material transfer agreements (MTAs) and limited use label licenses...

  11. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...listing of transfer status for viral preps.... Viral Vector Packaging Service Penn Vector Core Transfer Viral ...Viral Vector Packaging Service: Penn Vector Core Transfer The University of Pennsylvania Vector Core (Penn...inventory , which includes all viral vectors transferred from the Penn Vector Core. Chemogenetics (including...

  12. TALEN Guide

    Type
    Collection
    ..., that will probably never happen. Yet with transcription activator-like (TAL) effectors, scientists in... the plant bacterial pathogen Xanthomonas. The central TAL targeting domain is composed of 33-35 amino...sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Feb;29(2):149-53. PMID...

  13. Arf GTPase Family

    Type
    Collection
    ...Ettema, T. J. (2016). Tracing the archaeal origins of Eukaryotic Membrane-Trafficking System building blocks...known for their roles as regulators of membrane traffic, they also play critical roles at cilia, centrosomes...

  14. Brain Armamentarium

    Type
    Collection
    ...specimens. These technologies have the potential to transform neuroscience research by bringing the precision...enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells...interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent...

  15. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...using various delivery methods including intracerebroventricular (ICV) delivery in newborns, and retro-orbital...Tasic, B. (2025). A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell.... A., Larsen, R., Gore, B. B., Szelenyi, E. R., Trader, C., . . . Tasic, B. (2021). Enhancer viruses for...

  16. Antibodies

    Type
    Collection
    ...for recommended applications. Addgene values transparency and accessibility in research and therefore ...our production process? At Addgene, we provide transparency in our production process by sharing...

  17. KLF Research Plasmids

    Type
    Collection
    ...KLFs) are a family of at least 17 DNA binding transcriptional regulators with diverse roles in processes ...elements or GC-rich regions on DNA to mediate transcriptional activation and/or repression. Continuing collaborative...

  19. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...different cell types. Zinc finger arrays can be transferred in-frame to these vectors....either OPEN or modular assembly can be easily transferred in-frame to any of these vectors using unique...

  20. All Antibodies

    Type
    Collection
    ...currently available from our catalog. Addgene values transparency and accessibility in research. For each antibody... other difficult-to-target antigens that are intractable via animal immunization. Addgene's antibody catalog...
Showing: 81 - 100 of 123 results