...California, San Francisco Browse Plasmids ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...between the Cell Migration Consortium (CMC) and Addgene, which highlights CMC depositing labs and plasmids...collaboration between the Cell Migration Consortium and Addgene. Member laboratories of the Cell Migration Consortium...plasmids useful for cell migration research at Addgene. CMC investigators who have deposited plasmids ...

...scaffold to allow scientists to selectively modulate gene expression....Backbones FRET Subcellular localization Biosensors Optogenetics Background Fluorescent proteins such as GFP ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...used to create cell lines with endogenously-tagged gene variants or to differentiate human iPSCs into other...Find plasmids generated by the iPSC Neurodegenerative Disease Initiative (iNDI) here. Depositor...Stem Cell Research Neurodegeneration Research Collection The iPSC Neurodegenerative Disease Initiative...plasmid collection at Addgene. Figure 1: Schematic of iNDI iPSC line generation, phenotyping, and distribution...Collections iNDI iPSC Neurodegenerative Disease Initiative (iNDI) Collection You May Also Like... CRISPR ...Initiative (iNDI) project aims to generate and characterize single nucleotide variant lines in an isogenic cellular...NIH. Reference Ramos et al. 2021. Tackling neurodegenerative diseases with genomic engineering: A new stem...

...tags for you to create fusion proteins with your gene of interest. Please visit Michael Davidson's Molecular...Empty Backbones FRET Subcellular localization Optogenetics The lab of Michael Davidson from Florida State...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...example, choosing target sites that span the knock-in gene. For making mutations, one good way is to mutate...be okay if you are introducing a selection marker gene. The homology arms should be no more than 100bp ...for my genomic PCR, but couldn't amplify the EMX1 gene using same primer you used in the Science paper ...You may also like... Zhang Lab CRISPR plasmids General CRISPR Plasmids Dr. Le Cong, the first author of...additional PAM sequences should not be sufficient to generate off-target DSBs; there needs to be extensive homology...below are considerations for designing HR donor. Generally if off-target (non-specific) cleavage is not a...purified oligo is fine based on new data). For general transfection, you could use 1ul of 10uM oligo stock...

...editing in mammalian systems. Mammalian ID Plasmid Gene/Insert PI Publication Do you have suggestions for...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...SARS-CoV-2 and common coronvirus plasmids based on: Gene insert (e.g. spike, envelope, nsp3, OC43, NL63, ...Additional Resources Read Addgene's Blog Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids...for a summary of the Ginkgo collection at Addgene. Addgene's COVID-19 and Coronavirus Plasmids & Resources... viral proteins in several expression systems. Addgene has partnered with Ginkgo to make these plasmids...expressed or functionally validated by Ginkgo or Addgene. Plasmids Use the search bar or sort buttons to...to COVID-19 that may be of use to scientists. Addgene's Protocol for RNA Extraction Without A Kit...

... throughout the chromosome. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...faithfully introduce specific genomic changes. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 51306 pUC57-Simple-gRNA backbone T7...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Vector Type Promoter PI Publication Empty...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 46759 pT7-gRNA T7 BsmBI In vitro transcription...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Adeno-associated virus (AAV) vectors are safe and useful gene delivery tools that effectively transduce many mammalian...Packaging Service overview. Function Chemogenetics DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins...Ready-to-use adeno-associated virus (AAV) available from Addgene's viral vectory packaging service. Browse constructs...preps are purified by density centrifugation. Addgene’s comprehensive high-quality quality control process...shipment. If you can't find the prep you need, try Addgene's AAV Packaged on Request service. You can choose...

...you to create fusion proteins with your gene of interest....protein (GFP) was cloned in 1992 ( Prasher et al., Gene, 1992 ), and since then scientists have engineered...tags for you to create fusion proteins with your gene of interest. These are organized By Color of the...Lab Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha Lab Plasmids...Addgene has assembled a collection of empty plasmid backbones with different fluorescent tags for you...Monomer? Background Fluorescent proteins are genetically encoded tools that are used extensively by life...proteins that result in a diverse set of colors. Addgene has assembled a collection of empty plasmid backbones...

...AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter...Service: Chemogenetics AAV Blog: Chemogenetics Viral Vectors 101 Blog: Chemogenetics vs. Optogenetics Chemogenetics... more about chemogenetic resources available at Addgene. See our inventory of chemogenetic constructs,...Collections Chemogenetics Plasmids Chemogenetics Plasmid Collection You may also like... Chemogenetics Guide...biological processes. Addgene’s collection contains two main classes of chemogenetic receptors: Designer...luciferase and a light-sensing optogenetic element, making a bimodal opto-chemogenetic actuator. When the luciferase...your experiments, please see our Chemogenetics Guide . Chemogenetics Plasmids Browse, sort, or search ...

... of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression...engineering forms the core of the deorphanizing engine. Genetic and proteomic interactions represent the framework.... Protein production is used for assays and to generate antibody/ high-affinity ligands. Data is embedded...integrated knowledgebase that features, among others, genetic variants and disease associations. Plasmids This...overexpression pooled libraries are useful tools for genetic screening experiments. These libraries target the...

...an empty backbone can be repeated to ligate your gene of interest after the promoter, and so on. The following...

...site-specific recombinases, which can be used to control gene expression. See our Cre-lox Plasmids page for more...

...adenoviral plasmids. Want an adenoviral plasmid with your gene of interest? Search our database for adenoviral ...capacity (>8 kb), high titers, and high levels of transgene expression. Additionally, these viruses are able...Disadvantages of adenoviruses include transient transgene expression, as well as the substantial immune ...transfected into mammalian packaging cell lines to generate adenovirus particles. Read our Adenoviral Guide...terms Adenoviral Strains Bacterial strains for generating adenovirus. ID Strain Description PI 16398 BJ5183... Shuttle For production of viruses containing transgene under a chosen promoter Vogelstein 16403 pShuttle-CMV... Shuttle For production of viruses containing transgene under CMV promoter Vogelstein 16404 pAdTrack Shuttle...

...currently available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit name Type PI Description High...Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search Use Addgene's Search to...protection for material providers. Addgene’s deposit agreements allow Addgene to distribute plasmids on behalf...can be submitted to Addgene through your user account or by email to mta@addgene.org . After we review ...A portion of Addgene’s collection is available to qualified for-profit entities. Addgene reserves the ...Addgene has a growing collection of plasmids that are available to industry. Learn more about the available...assembling standardized parts to create eukaryotic multigene constructs. Pooled Libraries Pooled Library name...

...-mouse" or "anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions ...Find information on Addgene's collection of plasmid-based antibodies, including monoclonal antibodies...Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant antibodies...application-specific validation and quality control by Addgene as well as by our trusted partner labs. Learn More...animals or hybridoma cells, but are difficult to generate due to expense and time. An alternative approach...fragment libraries from chickens Methods for the generation of chicken monoclonal antibody fragments by phage... 2015 Oct;167(2):95-102. Joanna Bereta Sybody Generation Toolbox for making synthetic nanobodies (sybodies...

...opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases Addgene ID Plasmid...definitions (e.g. neurons defined by a single gene or projection). Intersectional expression approaches ... in well-defined cellular sub-populations has generally been limited to single-component cellular definitions...cells based on a doubly-specified combination of genetic and/or anatomical-defined parameters, by placing...Operating Procedure (Link opens in a new window) with general principles for working with INTRSECT recombinases...pAAV-CaMKIIa-Flpo None No Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral...Vcre No Dual recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral...