Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 88 results for: IRE

Showing: 61 - 70 of 88 results

  1. CRISPR Plasmids - Base Edit

    Type
    Collection
    ... not exist in nature, but have been created by directed evolution of the Escherichia coli TadA, a tRNA...wider editing window, and are thus well suited to directed evolution applications. Examples of these base...

  2. Promega Plasmid Collection

    Type
    Collection
    ...under research use only (RUO) terms and do not require an MTA. Pricing can be found on the individual ...tag enabling precise protein detection. It can be paired with LgBiT for bioluminescent assays or with the...

  3. TALEN Plasmids and Kits

    Type
    Collection
    ...higher than the parental vectors. pC-GoldyTALEN directs expression of TALENs from a truncated CAGs promoter...domains (DD, RR) used are obligate heterodimers that require cloning of left and right TALEN monomer proteins...

  4. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms and selection...Rep. 2015 . Alternatively, cDNAs can be cloned directly into this vector and targeted to the AAVS1 genomic...

  5. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... culture. Prep size may be scaled based on the required DNA yield. Perform maxi-prep for each CRISPR/Cas9...step 6 ). Select the clones identified with the desired deletion and move to larger plate or flask for ...

  6. Zhang Lab CRISPR Page

    Type
    Collection
    ...mutations in p53 and Lkb1 , as well as homology directed repair-mediated Kras G12D mutations, leading to...This plasmid also contains a Kras G12D homology directed repair donor template. #60225 - AAV:ITR-U6-sgRNA...

  7. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Two unique gRNAs, binding ~15-25 bp apart, are required for dCas9-FokI to dimerize at a given region of...

  9. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...CRISPR Activate and Interfere technologies also indirectly modify local chromatin state. Want more information...

  10. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ... is an A->G change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it...
Showing: 61 - 70 of 88 results