We narrowed to 44 results for: HA tag

...including Xenopus HA Epitope tag pcDNA3.1-HA - Mammalian expression vector with N-terminal HA tag pInducer20 ...fusion protein. Read more about epitope tags and protein tags . Tag or Fusion Protein Common uses...bacterial expression; His,GST tag pE2c - Modified pENTR vector for C-terminal 3x HA tag fusion with your gene ...Flag Epitope tag c-Flag pcDNA3 or FLAG-HA-pcDNA3.1 - C-terminal Flag or N-terminal FLAG-HA for mammalian...collection page Return to top Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools...stop codon for C-terminal tags and omit the start codon for N-terminal tags. And when you are designing... C-terminal tagging in S. cerevisiae pCS2FLAG - N- or C-terminal 2xFLAG tag in pCS for ...

... Lab TAP Tagging Protocol 97.2 KB Yamamoto PITCh Tagging System The Takashi Yamamoto lab has deposited...Förstemann Drosophila Cell Tagging System The Förstemann lab has developed a CRISPR tagging technique for use ... CRISPR Protein Tagging CRISPR Plasmids - Protein Tagging Browse... CRISPR tagging methods allow for improved efficiency and throughput over traditional tagging methods....design. How to use CRISPR to tag your gene of interest Mendenhall and Myers Tagging System The Eric Mendenhall...deposited plasmids in this CRISPR-Cas tagging system were tested by tagging transcription factors with FLAG ...Protocol 134.7 KB Mendenhall and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA ...

...nEF CAG E2 regulatory element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity...SYN1 HA-hM4D(Gi) hM4D(Gi) - Inhibition HA fusion none 2, 5 Christopher Richie 121539 pAAV SYN1 HA-hM3D... Roth 50454 pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine ... Roth 50455 pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine ...(Gq) - Activation HA fusion none 2, 5, rg* Christopher Richie 50467 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine...Gordon Fishell 50472 pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine rM3D(Gs) - Activation HA fusion and IRES mCitrine ...Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype PI 44361 pAAV-hSyn-DIO-hM3D...

...NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag SNAP-tag is based on the human O6...non-cell-permeant proteins. CLIP-tag CLIP-tag is identical in function to SNAP-tag but reacts with benzylcytosine...or localization. ACP and MCP tags ACP- and MCP-tags are small protein tags based on the acyl carrier protein...these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7) Vector SNAP-tag Empty ...Plasmid SNAP-tag Control for cell surface localization 101136 pCLIP-tag (m) Vector CLIP-tag Empty backbone...Plasmid CLIP-tag Control for cell surface localization 101127 pMCP-tag (m) Vector ACP/MCP-tag Empty backbone...and CLIP tag FAQs (PDF, 252 KB) ACP and MCP tags ACP-MCP-Tag plasmids - Substrate source (PDF, 46 KB) Please...

...Endogenous Gene Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells...with endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure...Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key cellular...You May Also Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization...collection (Link opens in a new window) of fluorescently-tagged human induced pluripotent stem cell (hiPSC) lines...researchers will be able to generate fluorescently tagged proteins of key cellular structures in human cell...TTN-mEGFP AICSDP-45 mEGFP Titin Sarcomeres (M-line tag) 114413 MYL7-mEGFP AICSDP-46 mEGFP MLC-2a Sarcomeric...

...Florida State University has contributed their comprehensive collection of ORFs tagged with a variety of fluorescent...Davidson lab has contributed the corresponding empty backbone with different fluorescent tags for you to...

...epitope tag or tags can be fused to dCas9 or gRNA for efficient purification. Various epitope tags including...including 3xFLAG-tag, PA, and biotin tags, can be used for enChIP, as well as an anti-Cas9 antibody. Biotin...Biotin tagging of dCas9 can be achieved by fusing a biotin acceptor site to dCas9 and co-expressing BirA...isolated by affinity purification against the epitope tag. Compared to conventional methods for genomic purification...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...

... mammalian) Tags (e.g. 6xHis, Flag, StrepII, HA, Myc, SUMO, GST, MBP, CBP, S-tag, TAP tag, TRX, or V5)...pseudovirus (Ctrunc) ID Plasmid Description Mutations Tags Industry Additional Addgene Resources Blog: Synthesized...

...Knock-in tagging - Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes...bearing either amino- or carboxyl-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish...tropical freshwater fish in the minnow family. It has long been established as a powerful vertebrate model...genes having an ortholog in zebrafish, this species has more recently emerged as a model system for human...Keith Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9...rapidly model potential cancer drivers in vivo. Biotagging toolkit - Tatjana Sauka-Spengler Lab. A genetic...

...constitutive Expression of ER-localized TurboID (with HA and V5 tag). 8 Jonathan Long 160857 pAAV-FLEx-ER-TurboID...Cre-dependent expression of ER-localized TurboID (with HA and V5 tag). 8 Jonathan Long Overexpression Tools These...pAAV-FLEX-EGFPL10a EF1a-driven and Cre-dependent EGFP-tagged ribosomal L10a. 5, rg* Nathaniel Heintz , Alexander...postsynaptic cells. 8 Don Arnold 232353 pAAV-DIO-BACE-HA Syn-driven, Cre-dependent Beta-Secretase to be expressed... Flp-dependent Flp-dependent expression of the voltage-gated Na+ channel mNaChBac and (physically separate...

...(RBPs) tagged with fluorescent proteins to visualize targeted genomic loci. CRISPR imaging has numerous...dynamics in living cells. The fluorescent CRISPR system has been used for dynamic tracking of repetitive and ...visualized in living cells using a single gRNA that has multiple targeted sequences in close proximity. A...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...numerous advantages over other imaging techniques, including that it is easy to implement due to the simplicity...

...DNMT1 with a Myc tag. (Li et al. J Biol Chem. 2006) Express human ATM with Flag and His tags. (Canman et al.... Express EGFP-tagged wt and mt tau . (Hoover et al. Neuron. 2010) Express EGFP-tagged wt and mt Alpha-synuclein...myelination. (Lam et al. Nat Commun. 2022) Endogenously tag gene variants for Alzheimer's disease and related...subcellular localization studies with GFP, or IP with Flag tag. (Bennett et al. Heliyon. 2020) Use VAP expression...Chemogenetics Plasmids . Find plasmids for endogenous gene tagging in human cells from the Allen Institute for Cell... Neurodegeneration Research Collection Addgene has assembled the following to help you find information...Parkinson's today. The Michael J. Fox Foundation has made a number of tools (Link opens in a new window...

...the repeat domain, and C-terminal SV40 NLS and HA tags. Plasmid 47389 also contains the VP64 domain (four...of a TALE, along with strong nuclear localisation tags and repress transcription initiation when targeted... plasmids 47388 and 47389 include a Flag epitope tag and an SV40 NLS at the N terminus, a 152-residue ...transfected cells, the system has successfully been employed for deletion mutagenesis and gene targeting in human...respectfully). This next generation architecture has been shown to increase mutation induction when using...

... (CAPTURE.) Tag : Multiple methods make it easier to tag endogenous loci with epitope tags or fluorescent...include mutations seen in human patients, protein tags, or loxP/FRT sites, among others. Homology-directed... not occur in mammalian cells. Purify : Epitope-tagged dCas9 can also be used to purify a genomic locus... EM, Myers RM. 2015. CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res . ...Technological Advances CRISPR technology development has occurred at a truly astonishing pace, with work directed...Directions It’s amazing to see how much progress has been made in just five years, with the skyrocketing...see results! Getting Started with CRISPR Addgene has empowered researchers to further develop the CRISPR...

...Applications Return to top Browse Epitope Tag Antibodies IPI has developed recombinant versions of antibodies...Cleft Antibodies NEW Integrin Antibodies Epitope Tag Antibodies Antibody Plasmids (Link opens in a new... (Neurexins and Neuroligins), and common epitope tags, as well as antibody plasmids to create or express...antibodies that recognize common epitope tags to help purify, detect, or localize particular proteins. Browse...His, Biotin, EE, GCN4 and Strep. Please visit our Tags and Other Markers Antibody Collection page to browse...s full catalog of antibodies that target epitope tags and other common cellular proteins. ID Recombinant...

...combinations of cell-filling antigenically distinct protein tags (or "bits"), creating over 100,000 unique combinations...detectable by antibodies. All of the PRISM protein tags have been validated for use in expansion microscopy...representation. To read out the barcodes, each protein tag can be imaged with a corresponding primary and dye-conjugated...al. (2025). PRISM Plasmids ID Plasmid Description Tags Additional Resources PRISM was developed by E11 ...CAG promoter (Addgene #242764–242781), but E11 Bio has also developed a set of Cre-dependent, hSyn-Tet inducible...

...DNA. The system can use common tags, like 3xFLAG-tag, PA, and biotin tags, or an anti-Cas9 antibody. The...in mammalian cells include: SunTag system — co-expression of epitope-tagged dCas9 and antibody-activator...acid, turn a promoter on/off, or add small protein tags. Precise Modifications Using Homology Directed Repair...Plasmids: Double-Strand Break (Cut) , Endogenous Tagging CRISPR Base Editing To overcome low HDR efficiency...Loci Using Fluorophores CRISPR imaging has numerous advantages, including the simplicity of gRNA design...also be fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR ...dCas9s (e.g. S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing...

...analogs Mammalian TAG Huiwang Ai 160041 pRaGE Pyl TAG GFP Y35TAG PylRS M. mazei Bacterial TAG Lital Alfonta...DiZHSeC Mammalian TAG P. Chen 92047 pCOTS-pyl-GFP(35TAG) PylRS M. mazei Cyanobacterial TAG Lital Alfonta ...Alfonta 160089 pPaGE Pyl TAG FliC T248TAG PylRS M. mazei Bacterial TAG Lital Alfonta 160377 pDule-Mb haloTyrRS...coli pBof Mammalian TAG Huiwang Ai 68292 SepOTSλ SepRS9 E. coli Sep Bacterial TAG Jesse Rinehart 71403...Cyanobacterial TAG Lital Alfonta 99222 pTECH-chPylRS(IPYE) PylRS Chimeric Nε-boc-lysine (BocK) Bacterial TAG Dieter...Bacterial TAG David Liu 104070 pTECH-MbAcK3RS(IPYE) AcK3RS M. barkeri Nε-acetyl-L-lysine Bacterial TAG David...Bacterial TAG David Liu 104072 pTECH-MbPylRS(IPYE) PylRS M. barkeri Nε-boc-lysine (BocK) Bacterial TAG David...

... gRNA backbones, and constructs for endogenous tagging. Some examples of useful worm CRISPR resources,...streamlines the production of targeting vectors for tag insertion using CRISPR/Cas9 and the selection of ...Better CRISPR/Cas9 Editing Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even more elegant...Editing in Caenorhabditis elegans. A modified approach has also been developed by the Fire laboratory and described...

...luciferase . Each type of luciferase has advantages and disadvantages depending on the application, and ...transcriptional reporters, but are less-than-ideal protein tags due to their large size (61 kDa and 36 kDa, respectively...Lentiviral expression of firefly luciferase with a V5 tag Kevin Janes 99283 pCW57.1-Luciferase Firefly TRE ...Mammalian expression of Nanoluc with a N-terminal Myc tag Erich Wanker 124701 pLenti-PGK-Venus-Akaluc (neo)...Lentiviral expression of Nanoluc with a N-terminal Myc tag Erich Wanker 115352 pFL-SV40 Firefly SV40 Mammalian... : Biosensor using Renilla luciferase and a Halo tag to assay mRNA turnover in real time. Nonsense-mediated...expression Renilla luciferase mutant Rluc8, which has increased stability and light output Sanjiv Sam Gambhir...