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We narrowed to 62 results for: Mammalian expression plasmids

Showing: 1 - 20 of 62 results

  1. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...pmEGFP-1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...pCEP4CyPet-MAMM - Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1...Bacterial Expression iLOV-N1 - Mammalian Expression iLOV-C1 - Mammalian Expression AcGFP1 475 505 28 Prone ...sfGFP-N1 - Mammalian Expression sfGFP-C1 - Mammalian Expression sfGFP-pBAD - Bacterial Expression moxGFP ...pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression...Topaz-N1 - Mammalian Expression Topaz-C1 - Mammalian Expression Topaz-pBAD - Bacterial Expression mTopaz ...

  2. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does.... Can express Cas9 with up to 3 gRNAs. Frew pSQT1313 Mammalian Single plasmid for the expression of multipe...requires expression of Csy4 (eg Plasmid 53369 ). Joung pX333 Mammalian Single all-in-one plasmid for tandem...Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut...maintained by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection...pSPgRNA 47108 Mammalian BbsI none S. pyogenes Gersbach pUC57-sgRNA expression vector 51132 Mammalian BsaI none...BPK2301 65778 Mammalian BsmBI none, need plasmid 65775 N. meningitidis Joung VVT1 65779 Mammalian BsmBI none...

  3. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...pAquaN1 Cyan Mammalian Expresses mammalian optimized Aquamarine mCerulean N1 Cyan Mammalian Express a gene ...pCEP4CyPet-MAMM Cyan Mammalian Expresses mammalian optimized CyPet pCyPet-His Cyan Bacterial Expresses CyPet with...pCEP4YPet-MAMM Yellow Mammalian Expresses mammalian optimized YPet pYPet-His Yellow Bacterial Expresses YPet with ...fluorescent protein. Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial Expresses Aquamarine with...Yellow Mammalian Express a gene of interest fused to the C-terminus of SYFP2 Clover Green Mammalian Expresses...N-terminus of monomeric Cerulean mCerulean C1 Cyan Mammalian Express a gene of interest fused to the C-terminus...with C-terminal His tag SCFP3A Cyan Mammalian Express a gene of interest fused to the C-terminus of SCFP3A...

  4. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Backbones Mammalian CMV, SV40, EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression... Empty mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry...C-terminal GFP for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2...Species-Specific Expression If you want to drive expression of your favorite gene, you will need a plasmid with...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent ... Expression - Rubin lab plasmids for Drosophila expression pMT-puro - Inducible...translation for mammalian expression pDest-527 - N-terminal His6 tag for bacterial expression (Gateway) pYD1...

  5. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...Consortium: Nuclease Expression Vectors This page lists plasmid vectors designed to express nucleases in different...domains: pST1374 (for expression in mammalian cells or zebrafish) pDW1775 (for expression in plants) Vectors...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays... Plasmid Collections Zinc Finger Consortium Nuclease Expression Vectors Zinc Finger...for expression of a “+” heterodimeric FokI domain. Miller et al., Nat Biotech 2007 ) in mammalian cells...for expression of a “-” heterodimeric FokI domain. Miller et al., Nat Biotech 2007 ) in mammalian cells..., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMLM802 (for expression of a ZFN targeted to a ...

  6. Genetic Code Expansion

    Type
    Collection
    ... you will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and its cognate...guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian cells. Plasmid...often used. The gene of interest can be expressed from a plasmid containing a UAG codon at the place where...different amino acid. They are typically expressed from a single plasmid, with multiple copies of the tRNA. ...Always express first with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian cells....Research Center . Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA ...synthetase for use in E.coli and Mammalian Cells. Many of the plasmids also contain one or more copies ...

  7. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...separate gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR... CRISPR Mammalian Expression CRISPR...NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function...your gene of interest. If the plasmid that you choose does not also express a gRNA, you will need to use...

  8. TALEN Plasmids and Kits

    Type
    Collection
    ...Maria-Elena Torres-Padilla These plasmids were designed for mammalian expression of fluorescent TAL effectors...The pTAL7 destination plasmids were optimized for TALEN expression in mammalian systems including hard-to-transfect...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description...-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built... For both plasmids sequence positions 1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting...Destination constructs were designed for optimal mammalian expression of Voytas Golden Gate-assembled TALEN, both...

  9. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...CRISPR prime editing plasmids. Plasmids are available for expression in mammalian systems, bacteria, plants...Drosophila. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...selectable marker, and whether the plasmid contains Cas9. ID Plasmid Expression System Promoter Guide RNA Type...Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert...Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6-pegRNA-GG-acceptor Mammalian hU6 pegRNA BsaI No...GG-acceptor Mammalian hU6 epegRNA BsaI No mRFP1 David Liu 174039 pU6-tmpknot-GG-acceptor Mammalian hU6 epegRNA...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  10. Luciferase Plasmid Collection

    Type
    Collection
    ...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...a reference level of expression. Browse or search for popular plasmids expressing luciferase. We also ... preparations of select luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description... CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression...of luciferase plasmids for gene expression assays and bioluminescent reporters. Plasmid... into a luciferase-containing plasmid and the effects on gene expression can be measured by the luminescence...

  11. Antibody Plasmid Collection

    Type
    Collection
    ...antibody components into plasmids and expressing these in bacteria, mammalian cells, yeast, plants, or... into a single mammalian expression vector A tool kit for rapid cloning and expression of recombinant ... Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies, nanobodies...Collections Antibody Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant...partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large proteins made...recombinant monoclonal antibodies (R-mAbs) expressed in mammalian cells. Monoclonal antibodies are derived...mAb Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells, the...

  12. Arf GTPase Family

    Type
    Collection
    ... Richard Kahn Lab Plasmids GTPase Plasmids GAP and GEF Plasmids References The ARF (ADP-ribosylation factor...examples of plasmid types available for each gene. Click the gene name for a full list of plasmids available... have plasmids available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arf3 377 181 Bacterial (pET), Mammalian (pcDNA3), Gateway...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arf5 381 180 Bacterial (pET), Mammalian (pcDNA3), Gateway...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl1 400 181 Bacterial (pET), Mammalian (pEGFP-N3) Gateway...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl3 403 182 Bacterial (pET), Mammalian (pcDNA3), Gateway...

  13. Tetracycline Inducible Expression

    Type
    Collection
    ...Promoters Mammalian shRNA Plasmids Collection Cre-lox Plasmids Collection Stem Cell Research Plasmids Collection...endogenous mammalian transcription factors to further minimize background expression. Improved expression of ...Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection. See Plasmid #21916 for ... other viral tTA plasmids. tTA Viviana Gradinaru 104102 pCAG-tTA Mammalian expression of tTA from the ...dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9...B19G ( Plasmid #100799 ). Ian Wickersham 172878 pSF3-ultraID Expression of UltraID in mammalian cells ...gene expression and find plasmids for your experiments using tet- or dox-inducible systems. Plasmid...

  14. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...CRISPR plasmids for transcriptional inhibition. Plasmids are available for expression in mammalian systems...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...of the coding sequence. If the plasmid you’re using does not also express a gRNA, you will need to use ...use a separate gRNA expression plasmid to target the dCas9-repressor to your specific locus. Want more information...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids for transcriptional repression of target genes. CRISPR...

  15. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...CRISPR plasmids for transcriptional activation. Plasmids are available for expression in mammalian systems...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...Activate CRISPR Plasmids: Activate Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick...your gene of interest. If the plasmid that you choose does not also express a gRNA, you will need to use...use a separate gRNA expression plasmid to target the dCas9-activator to your specific locus. Want more ...Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter...

  16. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Cell-Imaging Plasmid Collection New England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental...for stable and transient mammalian expression 101133 pSNAP-CaaX Control Plasmid SNAP-tag Control for endomembrane...for stable and transient mammalian expression 101134 pCLIPf-H2B Control Plasmid CLIP-tag Control for nuclear...for stable and transient mammalian expression 101132 pMCP-GPI Control Plasmid ACP/MCP-tag Control for ...Biolabs has deposited plasmids at Addgene to generate SNAP, CLIP, ACP or MCP tagged plasmids for visualization...synthases needed for use of these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7...Control Plasmid SNAP-tag Control for mitochondrial localization 101124 pSNAPf-H2B Control Plasmid SNAP-tag...

  17. TALEN Expression Vectors

    Type
    Collection
    ...Keith Joung Lab plasmids Daniel Voytas Lab plasmids CRISPR plasmids This page lists plasmid vectors into ...same). All expression vectors listed below have the CMV promoter for mammalian cell expression and a T7 ...Mammalian expression vectors for TAL effector repeat arrays assembled using the REAL, REAL-Fast or FLASH... TALengineering Reagents TALEN Expression Vectors TALEN Expression Vectors for REAL, REAL-Fast and FLASH...REAL-Fast or FLASH methods) can be cloned for expression as TALENs. Additional details about the REAL,...promoter for in vitro transcription of RNA. TALENs expressed from these vectors also have a FLAG epitope tag...localization signal at their amino-terminus. The expression vectors listed below can be purchased together...

  18. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...CRISPR plasmids to help purify genomic loci. Plasmids are available for expression in mammalian systems...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...Purify Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system...

  19. Mammalian RNAi Tools

    Type
    Collection
    ... Plasmid Collections Mammalian RNAi Mammalian shRNA Tools for RNAi Browse Plasmids Resources ...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...BioRender.com. shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a ...retroviral and AAV backbone options. Plasmids for constitutive expression as well as those that allow for ... (Cre-lox) or inducible (Tet) expression are available. To find plasmids containing other RNAi components...mutations in therapeutic settings. As a tool in mammalian cell systems, silencing is achieved through the... occur via transfection or viral delivery of a plasmid. shRNAs are often delivered using viral vectors...

  20. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ... CRISPR Cascade-Cas3 plasmids. Plasmids are available for expression in mammalian systems, bacteria, and... and plants. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Bacteria ID Plasmid Gene/Insert ...the Cascade complex to express equally. To combat this, some CRISPR-Cas3 plasmids take an all-in-one approach...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3...Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...
Showing: 1 - 20 of 62 results