We narrowed to 38 results for: cas9 genes

... sites for S. pyogenes Cas9, S. aureus Cas9, S. thermophilus Cas9, N. meningitidis Cas9, or Cas12a from...vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H Cas9 ; p201N Cas9 ; pUC gRNA Shuttle PDF, 500 KB...CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) PDF,... window) CRISPR/Cas9 reagent generation; gRNA design; HDR design; Deep sequencing Cas9: pMLM3613 dsDNA...CRISPR/Cas9 Knockout (MAGeCK). This tool can identify positively or negatively selected sgRNAs, genes, or...118 KB Church gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish...vitro transcription, injection gRNA core ; Cas9 ; optimized Cas9 PDF, 66.8 KB Fujii gRNA design and cloning...

...Return to top Cas9 SAM transcriptional activation plasmids and screening library CRISPR/Cas9 Synergistic... of endogenous genes. It consists of three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA...of cells and nuclei. Return to top AAV - Cas9 mouse CRISPR-Cas9 is a versatile genome editing technology...constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice the CRISPR-Cas9 system can be...constitutively Cas9-expressing mouse. Described here are AAV vectors that can be combined with Cas9 in a wide...are below. The Cas9 knockin mouse can be purchased from Jackson Labs: Cre-dependent Cas9 mouse Constitutively...cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors for...

... available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...dual nickases to generate a knockout or gene edit; or using Cas9 to generate large genomic deletions or ...strand SpCas9-HF1 — disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 — increase Cas9 proofreading...Plasmids: Purify Cas9 Alternatives for CRISPR Genome Engineering S. pyogenes Cas9 (SpCas9) is the most commonly...more Cas9 fusion tools later in the guide. Figure 3: Overview of Cas9n. Increasing fidelity of Cas9 Researchers...enhanced specificity by engineering Cas9 into high fidelity Cas9s (hfCas9) . There is currently no defined...application. Cas9 Orthologs While SpCas9 is the most commonly used Cas enzyme, additional Cas9 orthologs ...

...Additional Gene Silencing Resources A modular plasmid assembly kit for multigene expression, gene silencing...plant-optimized plasmids for cloning, gene expression, reporter assays, genome editing, gene silencing, and more. Plasmid...expressing different functional variants of Cas9. A CRISPR/Cas9 toolkit for multiplex genome editing in variety...sgRNA and Cas9 in rice . Includes a protocol (Link opens in a new window) for targeted gene mutation. ... a new window) — Tools for gene and protein expression and mapping of gene expression in plants. Grassius...Resources Cloning & SynBio Genome Editing Gene Silencing Reporters Addgene’s repository contains many plasmid ...from genome engineering and gene expression to reporter systems. Addgene’s collection includes plasmids...

...High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very ...III and Cas9 enzymes, which together separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease... anti-CRISPR genes in bacteriophages infecting Pseudomonas aeruginosa . Anti-CRISPR genes employ varied...Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs also have...especially amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are important...beyond SpCas9, moving us closer to being able to target every locus in every genome. Multiple Cas9 orthologs...capability of Cas9 to make other types of modifications at specific loci. Activate or Repress : dCas9 fused to...

...backbone. Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused...mouse genes along with 1000 non-targeting controls Puromycin Doench , Root Cas9 Lentivirus Cas9 viruses... 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate double-stranded...backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs targeting 19,114 human genes along with 1000 ...Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses are made from...backbone does not contain SpCas9. 76,441 unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting...

...can knock down genes by feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily...briggsae , etc.) gene or sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great... via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified Cas9, such as that...Tagging of Endogenous Genes with SapTrap Even more elegant: Single injection CRISPR/Cas9 in C. elegans External...Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. Mos1 Based Transgenesis miniMos... used in worms, search Addgene's entire collection. ID Plasmid Description Gene/Insert PI Plasmids Expressing...which diminishes epigenetic silencing in the germline. A "FLP-Out" system for controlled gene expression in...

...mutant allele of a gene (point mutant)? Increase or decrease expression of a target gene? Once you have a...). You will decide how to express Cas9, the delivery system for Cas9 and the guide RNA (gRNA), the design...non-homologous end joining (NHEJ) at the cut site Cas9 or Cas9 nickase High-fidelity Cas enzymes increase specificity...insertions, by copying from a DNA donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires...site. Consider alternative Cas9 enzymes with different PAM sequences or Cas9 enzymes with flexible PAM ... Target the promoter region of the gene of interest, where the Cas9 protein can block transcription from...targeting regions within the gene itself can also be effective, as the Cas9 bound to the DNA can interfere...

...are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase...editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has ...base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor. Both ...from the Michael Bassik lab , in which Cas9 is fused to activation-induced cytidine deaminase (AID). Figure...

...replace” gene editing method in which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert...contains Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection...directs the Cas9 nickase to the target sequence where it nicks the non-target strand and generates a 3’ flap...nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can be used in PE2...PE6 — small prime editors with optimized RT and/or Cas9 domains PE7 — addition of RNA-binding exonuclease...

...zebrafish gene of interest? Search Addgene's collection for plasmids that contain a zebrafish gene or sequence...Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector System... zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart research...zebrafish-specific gene expression database that provides information about when genes are active in developing...developing embryos. zfRegeneration - A database for gene expression profiling during regeneration. Seurat - R ...-type specific conditional gene inactivation - Jeffrey Essner Lab GeneWeld Vectors for Targeted Integration...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference...

...listed below. When targeting other gene loci, you will prepare the gene-specific CRISPR and donor vectors...pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific...Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which expresses an untagged Cas9 AND a gRNA...Drosophila cells that uses PCR to generate both an expression cassette for the Cas9-programming sgRNA and HR donors...multiple genes. Plasmids can be found associated with the following article: Paix, et al. Genetics 2014 Allen...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR...repair template design. How to use CRISPR to tag your gene of interest Mendenhall and Myers Tagging System ...

...Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI ...catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes, it generates a double-strand...PI Publication Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids... CRISPR dCas9-FokI CRISPR: dCas9-FokI...with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species...

...lab utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate gene targets. To accomplish...fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids... plasmids dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion...CRISPR Collection The Brzezinski lab investigates gene regulation in the context of the developing mouse...taken advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several...pX458 plasmid to expand its usefulness for the lab's gene regulation and developmental studies. Key plasmid...fate specification, yet differs from deleting both genes . Development. doi: 10.1242/dev.190272. PMID: 32541005...

...Mammalian ID Plasmid Gene/Insert Promoter PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter... genomic deletions. Compared to deletions generated by Cas9, large deletions created using Cas3 have fewer...Plant ID Plasmid Gene/Insert Promoter PI Publication Return to top CRISPR Resources Addgene has a large selection...underutilized compared to their Class 2 counterparts like Cas9. This is attributed in part to the difficulties ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Engineering Tools These AAV encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome editing...AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA CMV-driven, constitutive Expression of human codon-optimized Cas9 from Staphylococcus...Long Overexpression Tools These AAV encode various genes for overexpression. ID Name Expression System Activity...Ready-to-use AAV available from Addgene's viral service encoding tet-off transactivators and tools for...Staphylococcus aureus (SaCas9). 6, 8 Feng Zhang Labeling Tools These AAV encode tools that can be used...-DIO_EGFP-GFE3 Ef1a-driven, Cre-dependent For generating adeno-associated virus to express GFE3 under ...see Monosynaptic Neuronal Tracers available at Addgene. ID Name Expression System Activity Serotype PI...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert...Plant Yeast Resources Catalytically dead Cas9 (dCas9), or dCas9 fused to a transcriptional repressor peptide...plasmids for transcriptional repression of target genes. CRISPR...peptide like KRAB, can knock down gene expression by interfering with transcription. This interference...interference is most efficient when the gRNA targets your gene of interest’s promoter, enhancer, or the beginning... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...

...developments with CRISPR-Cas9 gene editing have propelled both reverse and forward genetics of these parasites...parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some of these parasites. Together... these tools have enabled precise genetic analyses to pinpoint genes linked to parasite virulence, transmission...predicted protein-coding genes. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors...content for this page was generated with the help of Scott Lindner . Cut ID Plasmid Gene/Insert Selectable Marker...plasmids that have been used in these efforts. Addgene's parasite CRISPR plasmid collection also includes... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert...researchers have fused catalytically dead Cas9 (dCas9) to epigenetic modifiers. Common modifiers include: ...methylation Epigenetic modification is typically targeted to a specific promoter or enhancer for your gene of ...CRISPR Epigenetics CRISPR Plasmids: Epigenetics Mammalian Plant Resources To make targeted epigenetic modifications... plasmids designed for epigenetic modification. To learn more about epigenetic modification with CRISPR...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and... local chromatin state. Figure 1: Overview of epigenetic modification using CRISPR. Created with BioRender.com...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert...Description Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has ...homology-directed repair (HDR). Figure 1: Overview of Cas9 nickase cleavage and repair. Browse, sort, or search...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...