...degradation of foreign nucleic acids. We have engineered the type II bacterial CRISPR system to function...plasmids is described in: RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, ...This set of plasmids is described in: Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas ...

...192824 Knockout Mouse Knouse 3rd ~5 71,878 Mouse messenger-RBP sgRNA Library 169082 Knockout Mouse Turner... gondii Lourido N/A 10 8,158 Turner Lab Human messenger-RBP sgRNA Library 168791 Knockout Human Turner...

... TALengineering Reagents TALEN Zebrafish Reagents TALENs for...Zebrafish Gene Targets Recent reports have shown that engineered TALENs can be used to induce targeted mutations...table below lists pairs of TALENs that have been engineered by the Joung, Peterson, and Yeh labs at MGH to...

... GFP Red-wavelength fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength fluorescent...

...oncogene homolog, avian) ERBB, ERBB1, HER1, PIG61, mENA ENG endoglin CD105, END, FLJ41744, HHT1, ORW, ORW1 EPGN...receptor (TCR) engages with antigen presenting cells of the innate immune system. Engagement of the TCR by...antigens through the B cell receptor (BCR). The strength and duration of signaling controls the fate of...tolerance or anergy are induced when the TCR is engaged without co-stimulation or with a low affinity antigen...

... Systems Bacterial Expression Systems Genome Engineering Visualization and Tagging Purification Controlled... expression systems to find out more! Genome Engineering Although overexpressing or knocking down a gene...purified protein. Protein purification can be challenging, but once you get it right, you can learn many...

...Research KLF Depositing Labs Juan Belmonte Linzhao Cheng Jose Cibelli George Daley Jin-Tang Dong Steven Dowdy...Townes Robert Weinberg Knut Woltjen Shinya Yamanaka Feng Zhang About The Krüppel-like factors (KLFs) are ...

... TALengineering Reagents TALEN Expression Vectors TALEN Expression... FLASH assembly methods can be found on the TALengineering website . All three of these methods yield ...

...CRISPR Casilio CRISPR/Cas Platform from JAX - Albert Cheng CRISPR-on: an RNA-guided transcriptional activator...Institute for Cell Science Plasmid Collection New England BioLabs Cell-Imaging Plasmid Collection Protein...

... TALengineering Reagents Transcriptional Activators TALE Transcriptional... FLASH assembly methods can be found on the TALengineering website . All three of these methods yield ...

...reagents for CRISPR/Cas9-based multiplex genome engineering in diverse cell types. This set of plasmids is...described in: Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Kabadi AM, Ousterout...

...immune system to a revolutionary tool for genome engineering. CRISPR Topic Page : Want the latest news on ...experiments. Browse CRISPR Plasmids by Function Genome Engineering Cut Wild-type Cas enzymes generate double strand...

...gondii Trypanosoma sp. Plasmids for Viruses Species Dengue Hepatitis Influenza Mumps virus Rubeola (measles...Read our CRISPR Methods for Bacterial Genome Engineering blog post to see how Addgene depositors are making...

...functionality or add new functions through genetic engineering and directed evolution. More information about...polymerase 165543 pOpen-Bstpol Bst DNA Polymerase, Full Length 165544 pOpen-T5gene122 T5 DNA Polymerase 165553...

...and non-coding RNAs in zebrafish - Tatjana Sauka-Spengler Lab Lineage Tracing Cell lineage tracing in multicellular...drivers in vivo. Biotagging toolkit - Tatjana Sauka-Spengler Lab. A genetic binary system for cell- and compartment-specific...

...phSyn1(S)-tdTomato-WPRE hSyn tdTomato Constitutive 5 Zeng 58909 pAAV-GFAP104-mCherry GFAP104 mCherry Constitutive...pCAG-FLEX-EGFP-WPRE CAG EGFP Cre dependent 1, 2, 5, 8, 9, rg* Zeng 59331 pAAV-CAG-FLEX-EGFP CAG EGFP Cre dependent ...

... Backbones Promoter Measure promoter strength pBV-Luc - Luciferase reporter plasmid... custom TALEN array for genomic engineering Do you have suggestions for other plasmids that...

...Finding nucleic acids with SHERLOCK and DETECTR . Feng Zhang's lab at the Broad Institute has utilized ...Center The Lancet COVID-19 Resource Centre The New England Journal of Medicine Coronavirus webpage PLOS Journals...

...re-purposed for site-specific eukaryotic genome engineering. CRISPR/Cas9 is an inexpensive, facile, and efficient...manuscript here: Bauer et al, 2014 322.2 KB Genome engineering using the CRISPR-Cas9 system. Ran FA...

...Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214. Cheng RNA Human Reprogramming factors for delivering synthetic...expression signatures. Cell Res. 2011 Mar;21(3):518-29. Cheng piggyBac Mouse Doxycycline-inducible piggyBac transposon...