...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...process of double nicking, however, increases indel formation slightly. Designing the sgRNA with a spacer that...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... recreate the diseases they cause. The standard format for providing individual plasmids is a bacterial...CRISPR Resources : Find essential background information on using CRISPR in your lab, specific CRISPR ...and gene regulation to sensing and signaling, to perform your synbio experiment. Synthetic Biology Assembly...Bacterial Expression Systems : Find plasmids to perform your experiment in a particular bacterial expression...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...self-excising drug selection cassette (SEC). More information regarding this kit can be found at the Goldstein...can be found on the Jorgensen Lab page. More information on miniMos and MosSCI plasmids can be found on...community with accurate, current, accessible information concerning the genetics, genomics and biology...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...gene, or browse the gene list below. For multiple isoforms or subunits, individual links to each gene page...plasmids for the gene. For components with multiple isoforms or subunits, individual links to each gene page...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...design of synthetic operons for chloroplast transformation and metabolic engineering in plants (including...set of functional parts for assembling plant transformation vectors - Daniel Voytas. Transcription Factors...Agrobacterium-mediated transient expression or plant transformation - Silin Zhong. RNAi The following collections...Editors Save Time with Transient Plant Leaf Transformations RNA Interference in Plant Biology: New Tools...thaliana and related species. The Arabidopsis Information Resource (TAIR) (Link opens in a new window) ...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...localization in high-throughput. MAZERATI platform - Leonard Zon Lab. A platform to rapidly model potential cancer...droplet reporters - Richard White Lab. An imaging platform to study lipid dynamics in vivo. Bimolecular fluorescence...repository for zebrafish strains and materials and information about zebrafish research. China Zebrafish Resource...zebrafish-specific gene expression database that provides information about when genes are active in developing embryos...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...gene, or browse the gene list below. For multiple isoforms or subunits, individual links to each gene page...plasmids for the gene. For components with multiple isoforms or subunits, individual links to each gene page...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...experiments to measure protein interactions or conformation changes, as well as FRET standards. Plasmid... intermolecular or bimolecular FRET) or (2) conformational changes within a protein where the donor and...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...

...see available in viral format by filling out our Viral Service Suggestion Form . Please note this does...Browse constructs currently available in lentiviral format. Viral...involved in specific biological processes. For more information, see our Guide to Plasmid Pooled Libraries . ... plasmids targeting specific genes. For more information on CRISPR plasmids and function, see our CRISPR...Activate Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate...

...Cell Junctions Cell junctions are responsible for forming connections and adhesions between animal cells....added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...

...CRISPR system consisting of a catalytically inactive form of Cas9 (dCas9) plus guide RNA (gRNA) for biochemical...biochemical purification (Fig. 2). For additional information and protocols, check our references for the plasmids...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd generation...transgene or shRNA. See article for detailed information about cloning & additional plasmids. Aebischer...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...expression for Gateway cloning; selection cassette in format: PGK-rtTA-2A-puro; see article for tagged insert...transgene or shRNA; see article for detailed information about cloning and additional plasmids tetR-KRAB...expression of a miR-shRNA; selection cassette in format: rtTA3+IRES+Neo; see article for additional selection...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...described below. Read our CRISPR 101 post for more information about HDR/repair template design. How to use ...

...Protein phosphorylation is one of the most abundant forms of posttranslational modifications in cells and ... is anticipated to be useful for laboratories performing phosphoproteomic experiments in need of reference...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...